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. 1992 Sep 1;176(3):781–786. doi: 10.1084/jem.176.3.781

Macrophage inflammatory protein-1 alpha activates basophils and mast cells

PMCID: PMC2119365  PMID: 1512541

Abstract

Macrophage inflammatory protein-1 (MIP) is a recently cloned cytokine that causes neutrophilic infiltration and induces an inflammatory response. We studied the effect of MIP-1 alpha on histamine secretion from basophils and mast cells. Leukocytes from allergic and normal subjects were studied. MIP-1 alpha caused dose-dependent release of histamine from basophils of 14 of 20 allergic donors at concentrations of 10(-9)-10(-7) M, and the mean release was 13.50 +/- 2.9% at the highest concentration. In the same experiments, the mean histamine release by anti-immunoglobulin E and monocyte chemotactic and activating factor (MCAF) (10(-7) M) was 32 +/- 7% and 31 +/- 3%, respectively. The cells from only 2 of 10 normal subjects released histamine in response to MIP-1 alpha. Histamine release by MIP-1 alpha was rapid, and almost complete within the first 3 min. MIP-1 alpha- induced degranulation was a calcium-dependent noncytotoxic process. MIP- 1 alpha showed chemotactic activity for purified basophils that was comparable to MCAF. Both MIP-1 alpha and MCAF at 10(-7) M concentration elicited a chemotactic response that was 40% of the maximal response to C5a (1 microgram/ml). Murine MIP-1 alpha induced histamine release from mouse peritoneal mast cells in a dose-dependent manner. Thus, we have established that MIP-1 alpha is a novel activator of basophils and mast cells.

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Selected References

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  1. Alam R., Kuna P., Rozniecki J., Kuzminska B. The magnitude of the spontaneous production of histamine-releasing factor (HRF) by lymphocytes in vitro correlates with the state of bronchial hyperreactivity in patients with asthma. J Allergy Clin Immunol. 1987 Jan;79(1):103–108. doi: 10.1016/s0091-6749(87)80023-4. [DOI] [PubMed] [Google Scholar]
  2. Alam R., Lett-Brown M. A., Forsythe P. A., Anderson-Walters D. J., Kenamore C., Kormos C., Grant J. A. Monocyte chemotactic and activating factor is a potent histamine-releasing factor for basophils. J Clin Invest. 1992 Mar;89(3):723–728. doi: 10.1172/JCI115648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alam R., Welter J. B., Forsythe P. A., Lett-Brown M. A., Grant J. A. Comparative effect of recombinant IL-1, -2, -3, -4, and -6, IFN-gamma, granulocyte-macrophage-colony-stimulating factor, tumor necrosis factor-alpha, and histamine-releasing factors on the secretion of histamine from basophils. J Immunol. 1989 May 15;142(10):3431–3435. [PubMed] [Google Scholar]
  4. Alam R., Welter J., Forsythe P. A., Lett-Brown M. A., Rankin J. A., Boyars M., Grant J. A. Detection of histamine release inhibitory factor- and histamine releasing factor-like activities in bronchoalveolar lavage fluids. Am Rev Respir Dis. 1990 Mar;141(3):666–671. doi: 10.1164/ajrccm/141.3.666. [DOI] [PubMed] [Google Scholar]
  5. Baeza M. L., Reddigari S. R., Kornfeld D., Ramani N., Smith E. M., Hossler P. A., Fischer T., Castor C. W., Gorevic P. G., Kaplan A. P. Relationship of one form of human histamine-releasing factor to connective tissue activating peptide-III. J Clin Invest. 1990 May;85(5):1516–1521. doi: 10.1172/JCI114598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Berman J. S., Center D. M. Chemotactic activity of porcine insulin for human T lymphocytes in vitro. J Immunol. 1987 Apr 1;138(7):2100–2103. [PubMed] [Google Scholar]
  7. Bischoff S. C., Krieger M., Brunner T., Dahinden C. A. Monocyte chemotactic protein 1 is a potent activator of human basophils. J Exp Med. 1992 May 1;175(5):1271–1275. doi: 10.1084/jem.175.5.1271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Bochner B. S., Luscinskas F. W., Gimbrone M. A., Jr, Newman W., Sterbinsky S. A., Derse-Anthony C. P., Klunk D., Schleimer R. P. Adhesion of human basophils, eosinophils, and neutrophils to interleukin 1-activated human vascular endothelial cells: contributions of endothelial cell adhesion molecules. J Exp Med. 1991 Jun 1;173(6):1553–1557. doi: 10.1084/jem.173.6.1553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Broide D. H., Smith C. M., Wasserman S. I. Mast cells and pulmonary fibrosis. Identification of a histamine releasing factor in bronchoalveolar lavage fluid. J Immunol. 1990 Sep 15;145(6):1838–1844. [PubMed] [Google Scholar]
  10. Conroy M. C., Adkinson N. F., Jr, lichtenstein L. M. Measurement of IgE on human basophils: relation to serum IgE and anti-IgE-induced histamine release. J Immunol. 1977 Apr;118(4):1317–1321. [PubMed] [Google Scholar]
  11. Davatelis G., Tekamp-Olson P., Wolpe S. D., Hermsen K., Luedke C., Gallegos C., Coit D., Merryweather J., Cerami A. Cloning and characterization of a cDNA for murine macrophage inflammatory protein (MIP), a novel monokine with inflammatory and chemokinetic properties. J Exp Med. 1988 Jun 1;167(6):1939–1944. doi: 10.1084/jem.167.6.1939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hook W. A., Siraganian R. P. Complement-induced histamine release from human basophils. III. Effect of pharmacologic agents. J Immunol. 1977 Feb;118(2):679–684. [PubMed] [Google Scholar]
  13. Kay A. B., Ying S., Varney V., Gaga M., Durham S. R., Moqbel R., Wardlaw A. J., Hamid Q. Messenger RNA expression of the cytokine gene cluster, interleukin 3 (IL-3), IL-4, IL-5, and granulocyte/macrophage colony-stimulating factor, in allergen-induced late-phase cutaneous reactions in atopic subjects. J Exp Med. 1991 Mar 1;173(3):775–778. doi: 10.1084/jem.173.3.775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Leonard E. J., Roberts R. L., Skeel A. Purification of human blood basophils by single step isopycnic banding on Percoll. J Leukoc Biol. 1984 Feb;35(2):169–177. doi: 10.1002/jlb.35.2.169. [DOI] [PubMed] [Google Scholar]
  15. MacDonald S. M., Lichtenstein L. M., Proud D., Plaut M., Naclerio R. M., MacGlashan D. W., Kagey-Sobotka A. Studies of IgE-dependent histamine releasing factors: heterogeneity of IgE. J Immunol. 1987 Jul 15;139(2):506–512. [PubMed] [Google Scholar]
  16. MacDonald S. M., Schleimer R. P., Kagey-Sobotka A., Gillis S., Lichtenstein L. M. Recombinant IL-3 induces histamine release from human basophils. J Immunol. 1989 May 15;142(10):3527–3532. [PubMed] [Google Scholar]
  17. Naclerio R. M., Proud D., Togias A. G., Adkinson N. F., Jr, Meyers D. A., Kagey-Sobotka A., Plaut M., Norman P. S., Lichtenstein L. M. Inflammatory mediators in late antigen-induced rhinitis. N Engl J Med. 1985 Jul 11;313(2):65–70. doi: 10.1056/NEJM198507113130201. [DOI] [PubMed] [Google Scholar]
  18. Nguyen K. L., Gillis S., MacGlashan D. W., Jr A comparative study of releasing and nonreleasing human basophils: nonreleasing basophils lack an early component of the signal transduction pathway that follows IgE cross-linking. J Allergy Clin Immunol. 1990 Jun;85(6):1020–1029. doi: 10.1016/0091-6749(90)90046-7. [DOI] [PubMed] [Google Scholar]
  19. Sampson H. A., Broadbent K. R., Bernhisel-Broadbent J. Spontaneous release of histamine from basophils and histamine-releasing factor in patients with atopic dermatitis and food hypersensitivity. N Engl J Med. 1989 Jul 27;321(4):228–232. doi: 10.1056/NEJM198907273210405. [DOI] [PubMed] [Google Scholar]
  20. Warner J. A., Pienkowski M. M., Plaut M., Norman P. S., Lichtenstein L. M. Identification of histamine releasing factor(s) in the late phase of cutaneous IgE-mediated reactions. J Immunol. 1986 Apr 1;136(7):2583–2587. [PubMed] [Google Scholar]
  21. Wolpe S. D., Cerami A. Macrophage inflammatory proteins 1 and 2: members of a novel superfamily of cytokines. FASEB J. 1989 Dec;3(14):2565–2573. doi: 10.1096/fasebj.3.14.2687068. [DOI] [PubMed] [Google Scholar]
  22. Wolpe S. D., Davatelis G., Sherry B., Beutler B., Hesse D. G., Nguyen H. T., Moldawer L. L., Nathan C. F., Lowry S. F., Cerami A. Macrophages secrete a novel heparin-binding protein with inflammatory and neutrophil chemokinetic properties. J Exp Med. 1988 Feb 1;167(2):570–581. doi: 10.1084/jem.167.2.570. [DOI] [PMC free article] [PubMed] [Google Scholar]

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