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. 1991 Jul;32(7):749–753. doi: 10.1136/gut.32.7.749

Abnormal permeability precedes the development of a gluten sensitive enteropathy in Irish setter dogs.

E J Hall 1, R M Batt 1
PMCID: PMC1378989  PMID: 1906829

Abstract

Intestinal permeability to 51Cr-EDTA was examined during the development of gluten sensitive enteropathy in dogs bred from affected Irish setters and reared on a normal wheat containing diet. Comparisons were made with litter mates reared on a gluten free diet and with a control group of age matched, clinically healthy Irish setters reared on the normal diet. Studies at 4, 6, 8, and 12 months of age were correlated with morphometric and biochemical examinations of peroral jejunal biopsy specimens. Permeability was increased at all ages in the group fed gluten free diet compared with control dogs, although there were no differences in villus height, intraepithelial lymphocyte density, and alkaline phosphatase activity. At four months, permeability in the normal diet group was greater than in controls, although comparable with that in the gluten free diet group. Permeability in the normal diet group increased further in conjunction with the development of partial villus atrophy and reduced alkaline phosphatase activity, and by 12 months permeability was significantly greater than in their gluten free diet litter mates and the control dogs. The findings suggest that an underlying permeability abnormality may be involved in the pathogenesis of gluten sensitive enteropathy in Irish setter dogs.

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Selected References

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  1. Ahrens F. A., Aronson A. L. A comparative study of the toxic effects of calcium and chromium chelates of ethylenediaminetetraacetate in the dog. Toxicol Appl Pharmacol. 1971 Jan;18(1):10–25. doi: 10.1016/0041-008x(71)90310-3. [DOI] [PubMed] [Google Scholar]
  2. Batt R. M., Carter M. W., McLean L. Morphological and biochemical studies of a naturally occurring enteropathy in the Irish setter dog: a comparison with coeliac disease in man. Res Vet Sci. 1984 Nov;37(3):339–346. [PubMed] [Google Scholar]
  3. Batt R. M., McLean L., Carter M. W. Sequential morphologic and biochemical studies of naturally occurring wheat-sensitive enteropathy in Irish setter dogs. Dig Dis Sci. 1987 Feb;32(2):184–194. doi: 10.1007/BF01297107. [DOI] [PubMed] [Google Scholar]
  4. Behrens R. H., Szaz K. F., Northrop C., Elia M., Neale G. Radionuclide tests for the assessment of intestinal permeability. Eur J Clin Invest. 1987 Apr;17(2):100–105. doi: 10.1111/j.1365-2362.1987.tb02387.x. [DOI] [PubMed] [Google Scholar]
  5. Bjarnason I., Marsh M. N., Price A., Levi A. J., Peters T. J. Intestinal permeability in patients with coeliac disease and dermatitis herpetiformis. Gut. 1985 Nov;26(11):1214–1219. doi: 10.1136/gut.26.11.1214. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bjarnason I., Peters J. Helping the mucosa make sense of macromolecules. Gut. 1987 Sep;28(9):1057–1061. doi: 10.1136/gut.28.9.1057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bjarnason I., Peters T. J., Veall N. 51Cr-EDTA test for intestinal permeability. Lancet. 1984 Sep 1;2(8401):523–523. doi: 10.1016/s0140-6736(84)92601-1. [DOI] [PubMed] [Google Scholar]
  8. Bjarnason I., Peters T. J., Veall N. A persistent defect in intestinal permeability in coeliac disease demonstrated by a 51Cr-labelled EDTA absorption test. Lancet. 1983 Feb 12;1(8320):323–325. doi: 10.1016/s0140-6736(83)91628-8. [DOI] [PubMed] [Google Scholar]
  9. Bourke S., Murphy B., Stafford F., Maher K., O'Morain C. Population differences in intestinal permeability to chromium EDTA. Ir J Med Sci. 1988 Sep;157(9):287–289. doi: 10.1007/BF02948314. [DOI] [PubMed] [Google Scholar]
  10. Bronstein H. D., Haeffner L. J., Kowlessar O. D. Enzymatic digestion of gliadin: the effect of the resultant peptides in adult celiac disease. Clin Chim Acta. 1966 Aug;14(2):141–155. doi: 10.1016/0009-8981(66)90080-5. [DOI] [PubMed] [Google Scholar]
  11. Cherbut C., Meirieu O., Ruckebusch Y. Effect of diet on intestinal xylose absorption in dogs. Dig Dis Sci. 1986 Apr;31(4):385–391. doi: 10.1007/BF01311674. [DOI] [PubMed] [Google Scholar]
  12. Davin J. C., Forget P., Mahieu P. R. Increased intestinal permeability to (51 Cr) EDTA is correlated with IgA immune complex-plasma levels in children with IgA-associated nephropathies. Acta Paediatr Scand. 1988 Jan;77(1):118–124. doi: 10.1111/j.1651-2227.1988.tb10609.x. [DOI] [PubMed] [Google Scholar]
  13. Dawson D. J., Lobley R. W., Burrows P. C., Notman J. A., Mahon M., Holmes R. Changes in jejunal permeability and passive permeation of sugars in intestinal biopsies in coeliac disease and Crohn's disease. Clin Sci (Lond) 1988 Apr;74(4):427–431. doi: 10.1042/cs0740427. [DOI] [PubMed] [Google Scholar]
  14. Ferry D. M., Butt T. J., Broom M. F., Hunter J., Chadwick V. S. Bacterial chemotactic oligopeptides and the intestinal mucosal barrier. Gastroenterology. 1989 Jul;97(1):61–67. doi: 10.1016/0016-5085(89)91416-9. [DOI] [PubMed] [Google Scholar]
  15. Fotherby K. J., Wraight E. P., Neale G. 51Cr-EDTA/14C-mannitol intestinal permeability test. Clinical use in screening for coeliac disease. Scand J Gastroenterol. 1988 Mar;23(2):171–177. doi: 10.3109/00365528809103963. [DOI] [PubMed] [Google Scholar]
  16. Hall E. J., Batt R. M., Brown A. Assessment of canine intestinal permeability, using 51Cr-labeled ethylenediaminetetraacetate. Am J Vet Res. 1989 Dec;50(12):2069–2074. [PubMed] [Google Scholar]
  17. Hall E. J., Batt R. M. Development of wheat-sensitive enteropathy in Irish Setters: biochemical changes. Am J Vet Res. 1990 Jul;51(7):983–989. [PubMed] [Google Scholar]
  18. Hall E. J., Batt R. M. Development of wheat-sensitive enteropathy in Irish Setters: morphologic changes. Am J Vet Res. 1990 Jul;51(7):978–982. [PubMed] [Google Scholar]
  19. Hamilton I., Cobden I., Rothwell J., Axon A. T. Intestinal permeability in coeliac disease: the response to gluten withdrawal and single-dose gluten challenge. Gut. 1982 Mar;23(3):202–210. doi: 10.1136/gut.23.3.202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Husby S., Foged N., Høst A., Svehag S. E. Passage of dietary antigens into the blood of children with coeliac disease. Quantification and size distribution of absorbed antigens. Gut. 1987 Sep;28(9):1062–1072. doi: 10.1136/gut.28.9.1062. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Juby L. D., Dixon M. F., Axon A. T. Abnormal intestinal permeability and jejunal morphometry. J Clin Pathol. 1987 Jul;40(7):714–718. doi: 10.1136/jcp.40.7.714. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Juby L. D., Rothwell J., Axon A. T. Cellobiose/mannitol sugar test--a sensitive tubeless test for coeliac disease: results on 1010 unselected patients. Gut. 1989 Apr;30(4):476–480. doi: 10.1136/gut.30.4.476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Juby L. D., Rothwell J., Axon A. T. Lactulose/mannitol test: an ideal screen for celiac disease. Gastroenterology. 1989 Jan;96(1):79–85. doi: 10.1016/0016-5085(89)90767-1. [DOI] [PubMed] [Google Scholar]
  24. Marsh M. N., Bjarnason I., Shaw J., Ellis A., Baker R., Peters T. J. Studies of intestinal lymphoid tissue. XIV--HLA status, mucosal morphology, permeability and epithelial lymphocyte populations in first degree relatives of patients with coeliac disease. Gut. 1990 Jan;31(1):32–36. doi: 10.1136/gut.31.1.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Martines D., Morris A. I., Gilmore I. T., Williams A., Stockdale H., Critchley M., Smith G. A., Billington D. Comparison between the cellobiose/mannitol and 51Cr-labelled ethylenediaminetetra-acetate absorption tests in the detection of coeliac disease. Clin Sci (Lond) 1988 Oct;75(4):375–378. doi: 10.1042/cs0750375. [DOI] [PubMed] [Google Scholar]
  26. Menzies I. S., Laker M. F., Pounder R., Bull J., Heyer S., Wheeler P. G., Creamer B. Abnormal intestinal permeability to sugars in villous atrophy. Lancet. 1979 Nov 24;2(8152):1107–1109. doi: 10.1016/s0140-6736(79)92507-8. [DOI] [PubMed] [Google Scholar]
  27. O'Mahony C. P., Stevens F. M., Bourke M., McCarthy C. F., Weir D. G., Feighery C. F. 51Cr-EDTA test for coeliac disease. Lancet. 1984 Jun 16;1(8390):1354–1355. doi: 10.1016/s0140-6736(84)91850-6. [DOI] [PubMed] [Google Scholar]
  28. Peled Y., Watz C., Gilat T. Measurement of intestinal permeability using 51Cr-EDTA. Am J Gastroenterol. 1985 Oct;80(10):770–773. [PubMed] [Google Scholar]
  29. Ramage J. K., Stanisz A., Scicchitano R., Hunt R. H., Perdue M. H. Effect of immunologic reactions on rat intestinal epithelium. Correlation of increased permeability to chromium 51-labeled ethylenediaminetetraacetic acid and ovalbumin during acute inflammation and anaphylaxis. Gastroenterology. 1988 Jun;94(6):1368–1375. [PubMed] [Google Scholar]
  30. Sanderson I. R., Boulton P., Menzies I., Walker-Smith J. A. Improvement of abnormal lactulose/rhamnose permeability in active Crohn's disease of the small bowel by an elemental diet. Gut. 1987 Sep;28(9):1073–1076. doi: 10.1136/gut.28.9.1073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Schacterle G. R., Pollack R. L. A simplified method for the quantitative assay of small amounts of protein in biologic material. Anal Biochem. 1973 Feb;51(2):654–655. doi: 10.1016/0003-2697(73)90523-x. [DOI] [PubMed] [Google Scholar]
  32. Stenhammar L., Fälth-Magnusson K., Jansson G., Magnusson K. E., Sundqvist T. Intestinal permeability to inert sugars and different-sized polyethyleneglycols in children with celiac disease. J Pediatr Gastroenterol Nutr. 1989 Oct;9(3):281–289. doi: 10.1097/00005176-198910000-00004. [DOI] [PubMed] [Google Scholar]
  33. Stenhammar L., Strömberg S. Intestinal permeability to lactulose/L-rhamnose in children with celiac disease and other gastrointestinal disorders. J Pediatr Gastroenterol Nutr. 1988 Mar-Apr;7(2):304–306. [PubMed] [Google Scholar]
  34. Stenling R., Fredrikzon B., Engberg S., Falkmer S. Surface ultrastructure of the small intestine mucosa in children with celiac disease. I. Untreated disease and effects of long-term gluten elimination and challenge. Ultrastruct Pathol. 1984;6(4):295–305. doi: 10.3109/01913128409018588. [DOI] [PubMed] [Google Scholar]
  35. Strobel S., Brydon W. G., Ferguson A. Cellobiose/mannitol sugar permeability test complements biopsy histopathology in clinical investigation of the jejunum. Gut. 1984 Nov;25(11):1241–1246. doi: 10.1136/gut.25.11.1241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Turck D., Ythier H., Maquet E., Deveaux M., Marchandise X., Farriaux J. P., Fontaine G. Intestinal permeability to [51Cr]EDTA in children with Crohn's disease and celiac disease. J Pediatr Gastroenterol Nutr. 1987 Jul-Aug;6(4):535–537. doi: 10.1097/00005176-198707000-00007. [DOI] [PubMed] [Google Scholar]
  37. Ukabam S. O., Cooper B. T. Small intestinal permeability as an indicator of jejunal mucosal recovery in patients with celiac sprue on a gluten-free diet. J Clin Gastroenterol. 1985 Jun;7(3):232–236. doi: 10.1097/00004836-198506000-00009. [DOI] [PubMed] [Google Scholar]

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