EMBO reports (2003) 4, 793–799
Corrigendum
In the August 2003 issue, a list of reagents used in the cell lysis buffer were published with incorrect concentrations. The correct final concentrations appear opposite (in the whole paragraph from the Methods).
Drosophila S2 cells were maintained in HyQCCM3 media (Perbio Science). We used the Effectene Transfection Reagent (Qiagen). Forty-eight hours post-transfection, cells were washed in PBS, spun at 3,000 r.p.m. and lysed on ice in lysis buffer (50 mM Hepes, 100 mM sodium chloride, 10 mM sodium fluoride, 5 mM EDTA, 0.5 mM sodium orthovanadate, 2 mM N-ethyl-maleimide, 0.1% Triton, and Complete protease inhibitors (Roche)). Western blots were performed using standard techniques. We used a mouse monoclonal antibody to GFP (Roche) followed by a horseradish peroxidase (HRP)-conjugated anti-mouse antibody (Jackson ImmunoResearch Laboratories).