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Highlighted in Zika Virus Collection

Combined proteome and transcriptome analyses reveal that Zika virus circulating in Brazil alters cell cycle and neurogenic programmes in human neurospheres

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1 D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil
2 Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
3 Department of Biochemistry and Tissue Biology, University of Campinas, Campinas, Brazil
4 Center for Technological Innovation, Evandro Chagas Institute, Belém, Brazil
5 Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
6 Department of Biochemistry and Tissue Biology, Laboratory of Neuroproteomics, University of Campinas (UNICAMP), Campinas, SP, Brazil
7 Institute Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil
8 Federal University of Pará, Belém, Brazil
DOI
10.7287/peerj.preprints.2033v1
Published
Accepted
Subject Areas
Cell Biology, Developmental Biology, Microbiology, Molecular Biology, Neuroscience
Keywords
Zika virus, Microcephaly, Neural stem cells, Neurospheres, Proteome, Transcriptome, Br ZIKV
Copyright
© 2016 Garcez et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Garcez PP, Minardi Nascimento J, Mota de Vasconcelos J, Madeiro da Costa R, Delvecchio R, Trindade P, Correia Loiola E, M. Higa L, Cassoli J, Vitória G, Sequeira P, Sochacki J, S. Aguiar R, Thais Fuzii H, M. Bispo de Filippis A, Lídio da Silva Gonçalves Vianez Júnior J, Martins-de-Souza D, Tanuri A, Rehen SK. 2016. Combined proteome and transcriptome analyses reveal that Zika virus circulating in Brazil alters cell cycle and neurogenic programmes in human neurospheres. PeerJ Preprints 4:e2033v1

Abstract

Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular and cellular consequences of Zika virus circulating in Brazil to the human brain development have not been studied yet. Here we describe alterations in human neurospheres derived from neural stem cells infected with Brazilian ZIKV. Combined proteomics and mRNA transcriptional profile analyses showed that Brazilian ZIKV, prior to induce cell death, alters cell cycle and halts neurogenic programmes, in addition to regulate transcription and protein translation due to viral replication. These results point to biological mechanisms potentially implicated in brain malformations as a result of ZIKV congenital infection.

Author Comment

This is a preprint submission to PeerJ Preprints and to a peer reviewed journal.

Supplemental Information

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Patricia P Garcez conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper.

Juliana Minardi Nascimento performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Janaina Mota de Vasconcelos performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Rodrigo Madeiro da Costa performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Rodrigo Delvecchio performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Pablo Trindade performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Erick Correia Loiola performed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper.

Luiza M. Higa performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Juliana Cassoli performed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper.

Gabriela Vitória performed the experiments, wrote the paper, reviewed drafts of the paper.

Patricia Sequeira performed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper.

Jaroslaw Sochacki performed the experiments, wrote the paper, reviewed drafts of the paper.

Renato S. Aguiar analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Hellen Thais Fuzii performed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper.

Ana M. Bispo de Filippis performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

João Lídio da Silva Gonçalves Vianez Júnior performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Daniel Martins-de-Souza performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Amilcar Tanuri analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Stevens K Rehen conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All protocols and procedures were approved by the institutional research ethics committee of Hospital Copa D'Or (CEPCOPADOR) under approved protocol # 727.269.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The obtained genome from BR_ZIKV_AB_ES isolate was deposited in GenBank with accession number KX212103.

Funding

Funds (not specifically for Zika virus studies) were provided by the Brazilian Development Bank (BNDES); Funding Authority for Studies and Projects (FINEP); National Council of Scientific and Technological Development (CNPq); Foundation for Research Support in the State of Rio de Janeiro (FAPERJ); the São Paulo Research Foundation (FAPESP, grants 14/21035-0, 14/14881-1, 13/08711-3 and 14/10068-4); and fellowships from the Coordination for the Improvement of Higher Education Personnel (CAPES). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Combined proteome and transcriptome analyses reveal that Zika virus circulating in Brazil alters cell cycle and neurogenic programmes in human neurospheres
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