Entry - 609467 - CHEMOKINE, CC MOTIF, LIGAND 3, PSEUDOGENE 1; CCL3P1 - OMIM
 
609467

CHEMOKINE, CC MOTIF, LIGAND 3, PSEUDOGENE 1; CCL3P1


Alternative titles; symbols

CHEMOKINE, CC MOTIF, LIGAND 3-LIKE 2; CCL3L2
LD78-GAMMA
G0S19-3
G0S19-GAMMA


HGNC Approved Gene Symbol: CCL3P1

Cytogenetic location: 17q12   Genomic coordinates (GRCh38) : 17:33,500,001-39,800,000


TEXT

Description

CCL3P1 is a truncated pseudogene similar to CCL3 (182283) and CCL3L1 (601395) (Hirashima et al., 1992).


Cloning and Expression

By examining restriction maps of genomic DNA that hybridized with a CCL3 cDNA probe, Blum et al. (1990) identified 3 putative genes, including CCL3L2, which they designated G0S19-3. The deduced protein contains at least 159 amino acids.

Hirashima et al. (1992) found that only 3 of 12 individuals tested carried the CCL3L2 gene, which they designated LD78-gamma. Analysis of a genomic clone indicated that the CCL3L2 sequence is similar to exons 2 and 3 of CCL3 and CCL3L1. Since CCL3L2 appears to contain a 5-prime truncation compared with CCL3 and CCL3L1, Hirashima et al. (1992) concluded that CCL3L2 is a pseudogene.


Gene Structure

Blum et al. (1990) determined that the CCL3L2 gene contains 2 exons that are homologous to exons 2 and 3 of the CCL3 and CCL3L1 genes.

Russell and Forsdyke (1993) sequenced a genomic clone of CCL3L2 and found that its 2 exons are joined to a 'CpG island-containing upstream sequence,' or CUS. The CUS contains potentially powerful promoter and enhancer elements. Russell and Forsdyke (1993) identified potential binding sites for numerous transcription factors, a Donehower conserved repetitive element, and motifs characteristic of cytokine, oncogene, and retroviral promoters. They hypothesized that the CUS-CCL3L2 sequence was generated by recombination between the 3 potential exons of the CUS sequence and the last 2 exons of a duplicated CCL3L1 gene.


Mapping

By Southern blot analysis of human-mouse somatic cell hybrids and in situ hybridization, Hirashima et al. (1992) mapped the CCL3L2 gene to chromosome 17q21.1-q21.3, close to CCL3 and CCL3L1.

Gross (2021) mapped the CCL3P1 pseudogene to chromosome 17q12 based on an alignment of the CCL3P1 sequence (GenBank D12592) with the genomic sequence (GRCh38).


REFERENCES

  1. Blum, S., Forsdyke, R. E., Forsdyke, D. R. Three human homologs of a murine gene encoding an inhibitor of stem cell proliferation. DNA Cell Biol. 9: 589-602, 1990. [PubMed: 2271120, related citations] [Full Text]

  2. Gross, M. B. Personal Communication. Baltimore, Md. 8/17/2021.

  3. Hirashima, M., Ono, T., Nakao, M., Nishi, H., Kimura, A., Nomiyama, H., Hamada, F., Yoshida, M. C., Shimada, K. Nucleotide sequence of the third cytokine LD78 gene and mapping of all three LD78 gene loci to human chromosome 17. DNA Seq. 3: 203-212, 1992. [PubMed: 1296815, related citations] [Full Text]

  4. Russell, L., Forsdyke, D. R. The third human homolog of a murine gene encoding an inhibitor of stem cell proliferation is truncated and linked to a CpG island-containing upstream sequence. DNA Cell Biol. 12: 157-175, 1993. [PubMed: 8097094, related citations] [Full Text]


Contributors:
Matthew B. Gross - update : 08/17/2021
Creation Date:
Patricia A. Hartz : 7/11/2005
mgross : 08/17/2021
mgross : 07/11/2005

609467

CHEMOKINE, CC MOTIF, LIGAND 3, PSEUDOGENE 1; CCL3P1


Alternative titles; symbols

CHEMOKINE, CC MOTIF, LIGAND 3-LIKE 2; CCL3L2
LD78-GAMMA
G0S19-3
G0S19-GAMMA


HGNC Approved Gene Symbol: CCL3P1

Cytogenetic location: 17q12   Genomic coordinates (GRCh38) : 17:33,500,001-39,800,000


TEXT

Description

CCL3P1 is a truncated pseudogene similar to CCL3 (182283) and CCL3L1 (601395) (Hirashima et al., 1992).


Cloning and Expression

By examining restriction maps of genomic DNA that hybridized with a CCL3 cDNA probe, Blum et al. (1990) identified 3 putative genes, including CCL3L2, which they designated G0S19-3. The deduced protein contains at least 159 amino acids.

Hirashima et al. (1992) found that only 3 of 12 individuals tested carried the CCL3L2 gene, which they designated LD78-gamma. Analysis of a genomic clone indicated that the CCL3L2 sequence is similar to exons 2 and 3 of CCL3 and CCL3L1. Since CCL3L2 appears to contain a 5-prime truncation compared with CCL3 and CCL3L1, Hirashima et al. (1992) concluded that CCL3L2 is a pseudogene.


Gene Structure

Blum et al. (1990) determined that the CCL3L2 gene contains 2 exons that are homologous to exons 2 and 3 of the CCL3 and CCL3L1 genes.

Russell and Forsdyke (1993) sequenced a genomic clone of CCL3L2 and found that its 2 exons are joined to a 'CpG island-containing upstream sequence,' or CUS. The CUS contains potentially powerful promoter and enhancer elements. Russell and Forsdyke (1993) identified potential binding sites for numerous transcription factors, a Donehower conserved repetitive element, and motifs characteristic of cytokine, oncogene, and retroviral promoters. They hypothesized that the CUS-CCL3L2 sequence was generated by recombination between the 3 potential exons of the CUS sequence and the last 2 exons of a duplicated CCL3L1 gene.


Mapping

By Southern blot analysis of human-mouse somatic cell hybrids and in situ hybridization, Hirashima et al. (1992) mapped the CCL3L2 gene to chromosome 17q21.1-q21.3, close to CCL3 and CCL3L1.

Gross (2021) mapped the CCL3P1 pseudogene to chromosome 17q12 based on an alignment of the CCL3P1 sequence (GenBank D12592) with the genomic sequence (GRCh38).


REFERENCES

  1. Blum, S., Forsdyke, R. E., Forsdyke, D. R. Three human homologs of a murine gene encoding an inhibitor of stem cell proliferation. DNA Cell Biol. 9: 589-602, 1990. [PubMed: 2271120] [Full Text: https://doi.org/10.1089/dna.1990.9.589]

  2. Gross, M. B. Personal Communication. Baltimore, Md. 8/17/2021.

  3. Hirashima, M., Ono, T., Nakao, M., Nishi, H., Kimura, A., Nomiyama, H., Hamada, F., Yoshida, M. C., Shimada, K. Nucleotide sequence of the third cytokine LD78 gene and mapping of all three LD78 gene loci to human chromosome 17. DNA Seq. 3: 203-212, 1992. [PubMed: 1296815] [Full Text: https://doi.org/10.3109/10425179209034019]

  4. Russell, L., Forsdyke, D. R. The third human homolog of a murine gene encoding an inhibitor of stem cell proliferation is truncated and linked to a CpG island-containing upstream sequence. DNA Cell Biol. 12: 157-175, 1993. [PubMed: 8097094] [Full Text: https://doi.org/10.1089/dna.1993.12.157]


Contributors:
Matthew B. Gross - update : 08/17/2021

Creation Date:
Patricia A. Hartz : 7/11/2005

Edit History:
mgross : 08/17/2021
mgross : 07/11/2005