Alternative titles; symbols
HGNC Approved Gene Symbol: FBXO2
Cytogenetic location: 1p36.22 Genomic coordinates (GRCh38) : 1:11,648,387-11,654,429 (from NCBI)
For background information on F-box proteins, see 605648.
Using a yeast 2-hybrid screen with SKP1 as bait, followed by searching sequence databases, Winston et al. (1999) and Cenciarelli et al. (1999) identified 33 mammalian and 26 human F-box proteins, respectively. These contained C termini with leucine-rich repeats (FBXLs, e.g., SKP2; 601436), WD40 domains (FBXWs, e.g., BTRCP; 603482), or no recognizable motifs (FBXOs, e.g., CCNF; 600227).
Erhardt et al. (1998) detected expression of the rat homolog of FBXO2, Nfb42, in all major areas of adult rat brain but not in nonneuronal tissue.
Jin et al. (2004) reported that the FBXO2 protein contains an F box in its N-terminal half, followed by a central F box-associated domain.
Using a yeast 2-hybrid screen of a HeLa cell cDNA library, Eom and Lehman (2002) identified an interaction of FBXO2 with neurotropic herpes simplex virus type-1 origin-binding protein (UL9 protein).
Yoshida et al. (2002) identified Fbx2 and Skp1 (601434) in a screen of mouse brain extracts with the N-glycan GlcNAc-terminated fetuin. Fbx2 was found to bind specifically to proteins such as glycosylated pre-integrin beta-1 (see 135630) attached to N-linked high-mannose oligosaccharides and to subsequently contribute to ubiquitination of N-glycosylated proteins as an E3 ubiquitin ligase through its F-box domain. Immunoblot analysis showed interaction with glycosylated CFTR (602421). Pulse-chase analysis revealed that CFTR degradation in the endoplasmic reticulum-associated degradation (ERAD) pathway requires the F-box domain of Fbx2. Yoshida et al. (2002) proposed that FBX2 is an E3 ubiquitin ligase specific for N-linked glycoproteins in the ERAD pathway and that it contributes to the rapid elimination of glycoproteins present in neurons.
Ilyin et al. (2002) determined that the FBXO2 gene contains 6 exons and spans about 5.5 kb.
By genomic sequence analysis, Ilyin et al. (2002) mapped the FBXO2 gene to chromosome 1p36, where it lies in tandem with the FBXO6 (605647) and FBXO44 (609011) genes.
Jin et al. (2004) stated that the mouse Fbxo2 gene maps to chromosome 4E2.0.
Cenciarelli, C., Chiaur, D. S., Guardavaccaro, D., Parks, W., Vidal, M., Pagano, M. Identification of a family of human F-box proteins. Curr. Biol. 9: 1177-1179, 1999. [PubMed: 10531035] [Full Text: https://doi.org/10.1016/S0960-9822(00)80020-2]
Eom, C. Y., Lehman, I. R. The human DnaJ protein, hTid-1, enhances binding of a multimer of the herpes simplex virus type 1 UL9 protein to oris, an origin of viral DNA replication. Proc. Nat. Acad. Sci. 99: 1894-1898, 2002. [PubMed: 11854491] [Full Text: https://doi.org/10.1073/pnas.042689499]
Erhardt, J. A., Hynicka, W., DiBenedetto, A., Shen, N., Stone, N., Paulson, H., Pittman, R. N. A novel F box protein, NFB42, is highly enriched in neurons and induces growth arrest. J. Biol. Chem. 273: 35222-35227, 1998. [PubMed: 9857061] [Full Text: https://doi.org/10.1074/jbc.273.52.35222]
Ilyin, G. P., Serandour, A.-L., Pigeon, C., Rialland, M., Glaise, D., Guguen-Guillouzo, C. A new subfamily of structurally related human F-box proteins. Gene 296: 11-20, 2002. [PubMed: 12383498] [Full Text: https://doi.org/10.1016/s0378-1119(02)00867-3]
Jin, J., Cardozo, T., Lovering, R. C., Elledge, S. J., Pagano, M., Harper, J. W. Systematic analysis and nomenclature of mammalian F-box proteins. Genes Dev. 18: 2573-2580, 2004. [PubMed: 15520277] [Full Text: https://doi.org/10.1101/gad.1255304]
Winston, J. T., Koepp, D. M., Zhu, C., Elledge, S. J., Harper, J. W. A family of mammalian F-box proteins. Curr. Biol. 9: 1180-1182, 1999. [PubMed: 10531037] [Full Text: https://doi.org/10.1016/S0960-9822(00)80021-4]
Yoshida, Y., Chiba, T., Tokunaga, F., Kawasaki, H., Iwai, K., Suzuki, T., Ito, Y., Matsuoka, K., Yoshida, M., Tanaka, K., Tai, T. E3 ubiquitin ligase that recognizes sugar chains. Nature 418: 438-442, 2002. [PubMed: 12140560] [Full Text: https://doi.org/10.1038/nature00890]