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Effects of hnRNP A2/B1 Knockdown on Inhibition of Glioblastoma Cell Invasion, Growth and Survival

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Abstract

Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) plays an important role in influence of pre-messenger RNA (pre-mRNA) processing and mRNA metabolism and transportation in cells. Increasing evidence indicates that hnRNP A2/B1 played an important role in development and progression of various human cancers. Forty cases of normal and human glioma tissue samples were analyzed using immunohistochemistry to reveal the expression of hnRNP A2/B1 protein in the samples. Then, knockdown of hnRNP A2/B1 expression induced by RNA interference (RNAi) method was used to analyze the role of hnRNP A2/B1 in glioblastoma cell viability, adhesion, migration, invasion, and chemoresistance for temozolomide (TMZ). The data showed that hnRNP A2/B1 protein was overexpressed in glioma tissue specimens and associated with advanced glioma grades. Knockdown of hnRNP A2/B1 could reduce glioblastoma cell viability, adhesion, migration, invasion, and chemoresistance for TMZ capacity, but induced tumor cells to apoptosis and reactive oxygen species (ROS) generation in glioma U251 and SHG44 cells. Molecularly, hnRNP A2/B1 knockdown reduced expression of phospho-STAT3 and MMP-2. Detection of hnRNP A2/B1 expression may be useful as a biomarker for prediction of glioma progression and knockdown of hnRNP A2/B1 expression as a novel strategy in future control of glioblastoma in clinic.

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Grant Support

This work is supported by the National Natural Science Foundation of China (No. 30870723 and 81201066) and Chongqing Natural Science Foundation Project (No. CSTC2013jcyjA10054).

Conflict of Interest

No potential conflicts of interest were disclosed.

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Authors and Affiliations

  1. Department of Neurosurgery, Children’s Hospital of Chongqing Medical University, Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, 400014, People’s Republic of China

    Jinmu Deng, Ping Liang & Xuan Zhai

  2. Department of Neurosurgery, First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, People’s Republic of China

    Song Chen

  3. Department of Neurosurgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, People’s Republic of China

    Feng Wang, Zongyi Xie, Zhongye Xu & Yuan Cheng

  4. Department of Neurosurgery, First Affiliated Hospital, Zunyi Medical College, Zunyi, 563003, People’s Republic of China

    Hongxin Zhao

  5. Department of Neurosurgery, Chongqing Emergency Medical Center, Chongqing, 400014, People’s Republic of China

    Qingtao Zhang

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  1. Jinmu Deng
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  10. Yuan Cheng
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Correspondence to Yuan Cheng.

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Deng, J., Chen, S., Wang, F. et al. Effects of hnRNP A2/B1 Knockdown on Inhibition of Glioblastoma Cell Invasion, Growth and Survival. Mol Neurobiol 53, 1132–1144 (2016). https://doi.org/10.1007/s12035-014-9080-3

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  • DOI: https://doi.org/10.1007/s12035-014-9080-3

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