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Hemolysate-induced Expression of Intercellular Adhesion Molecule-1 and Monocyte Chemoattractant Protein-1 Expression in Cultured Brain Microvascular Endothelial Cells via Through ROS-dependent NF-κB Pathways

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Abstract

In order to determine the possible effects of hemolysate on brain microvascular endothelial cells (BMECs), we examined the effects of hemolysate on the expression of intercellular adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein-1 (MCP-1), generation of reactive oxygen species (ROS), and NF-κB activation in rat BMECs. Hemolysate induced the expression of ICAM-1 and MCP-1 in endothelial cells. In addition, hemolysate stimulated nuclear translocation of the p65 subunit of NF-κB, and NF-κB DNA-binding activity in BMECs. Furthermore, hemolysate increased ROS generation, and hemolysate-induced ICAM-1and MCP-1 expression and NF-κB activation were abrogated in the presence of the direct scavenger of ROS. Taken together, our results indicate that hemolysate can induce inflammatory responses that increase expression of ICAM-1 and MCP-1, through ROS-dependent NF-κB activation in BMECs.

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Acknowledgment

This work was supported by a grant from the National Natural Science Foundation of China (no. 30700866).

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Authors and Affiliations

  1. Department of Neurosurgery, Jinling Hospital, Clinical School of Nanjing University, 305 East Zhongshan Road, Nanjing, 210002, China

    Hua Lu, Ji-Xin Shi, Yi-Xing Lin, Chun-Hua Hang & Hong-Xia Yin

  2. The State Key Laboratory of Pharmaceutical Biotechnology and Department of Biochemistry, College of Life Sciences, Nanjing University, Nanjing, 210093, China

    Dong-Mei Zhang & Jie Shen

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  1. Hua Lu
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Correspondence to Ji-Xin Shi.

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Lu, H., Shi, JX., Zhang, DM. et al. Hemolysate-induced Expression of Intercellular Adhesion Molecule-1 and Monocyte Chemoattractant Protein-1 Expression in Cultured Brain Microvascular Endothelial Cells via Through ROS-dependent NF-κB Pathways. Cell Mol Neurobiol 29, 87–95 (2009). https://doi.org/10.1007/s10571-008-9300-3

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