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Fusarium circinatum isolates from northern Spain are commonly infected by three distinct mitoviruses

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Abstract

Pitch canker is a serious disease of pines caused by the ascomycete fungus Gibberella circinata (anamorph = Fusarium circinatum). Three distinct mitovirus strains have been described in this fungus: Fusarium circinatum mitovirus 1 (FcMV1), FcMV2-1 and FcMV2-2. Here, we investigated the frequency and population variation of these viruses and closely related sequence variants in northern Spain using RT-PCR and sequencing. Each virus strain and similar sequence variants shared >95 % sequence identity and were collectively designated as virus types. All virus types were relatively common in Spain, with estimated prevalence of 18.5 %, 8.9 % and 16.3 % for FcMV1, FcMV2-1 and FcMV2-2, respectively.

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Acknowledgements

This study was financially supported by the Academy of Finland (decision numbers 251193 and 258520), the Spanish Ministry of Economy and Competitiveness (Project: AGL2012-39912), the Government of Cantabria, EU COST Actions FP1002 and FA1103 and the University of Valladolid. Instituto Agroforestal Mediterráneo and the Forestry and Agricultural Biotechnology Institute of the University of Pretoria provided strains for mitovirus screening. We are grateful to M.-L. Santanen, S. Sarsila, E. Simón Vicente, M. de Vallejo and J. Blanco for technical assistance, and Prof. M. Wingfield for commenting on the manuscript.

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Authors and Affiliations

  1. Natural Resources Institute Finland (Luke), Bio-based Business and Industry, Bioprocessing, P.O. Box 18, Jokiniemenkuja 1, 01301, Vantaa, Finland

    Eeva J. Vainio & Jarkko Hantula

  2. Sustainable Forest Management Research Institute, University of Valladolid-INIA, Avenida de Madrid 44, 34071, Palencia, Spain

    Pablo Martínez-Álvarez, Diana Bezos & Julio J. Diez

  3. Departamento de Producción Forestal y Recursos Forestales, University of Valladolid, Avenida de Madrid 44, 34071, Palencia, Spain

    Pablo Martínez-Álvarez, Diana Bezos & Julio J. Diez

Authors
  1. Eeva J. Vainio
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  2. Pablo Martínez-Álvarez
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  3. Diana Bezos
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  4. Jarkko Hantula
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  5. Julio J. Diez
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Corresponding author

Correspondence to Eeva J. Vainio.

Additional information

Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession number(s) KP726378–KP726394.

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Vainio, E.J., Martínez-Álvarez, P., Bezos, D. et al. Fusarium circinatum isolates from northern Spain are commonly infected by three distinct mitoviruses. Arch Virol 160, 2093–2098 (2015). https://doi.org/10.1007/s00705-015-2462-7

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  • DOI: https://doi.org/10.1007/s00705-015-2462-7

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