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Cloning of the herpes simplex virus type 1 genome as a novel luciferase-tagged infectious bacterial artificial chromosome

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Abstract

Herpes simplex virus type 1 (HSV-1) is a ubiquitous human pathogen of skin and mucous membranes. In the present study, the genome of the HSV-1 F strain was cloned as an infectious bacterial artificial chromosome (BAC) clone without any deletions of the viral genes. Additionally, a firefly luciferase cassette was inserted to generate a novel luciferase-expressing HSV-1 BAC. Importantly, the resulting recombinant HSV-1 BAC Luc behaved indistinguishably from the wild-type virus in Vero cells, and the luciferase activity could be easily quantified in vitro. Thus, this novel HSV-1 BAC system would serve as a powerful tool for gene function profiling.

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Acknowledgments

The authors are grateful to Dr. Cun Zhang, Zhejiang Academy of Agriculture Sciences, for helpful technical advice. This work was supported by grants from the Major State Basic Research Development Program of China (973 Program) (2011CB504802 and 2010CB530105).

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Authors and Affiliations

  1. Molecular Virology and Viral Immunology Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, 44 Xiaohongshan, Wuchang, Wuhan, 430071, People’s Republic of China

    You Li, Shuai Wang & Chunfu Zheng

  2. Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, 225 Warren Street, Newark, NJ, 07101, USA

    Hua Zhu

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  1. You Li
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Correspondence to Chunfu Zheng.

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Li, Y., Wang, S., Zhu, H. et al. Cloning of the herpes simplex virus type 1 genome as a novel luciferase-tagged infectious bacterial artificial chromosome. Arch Virol 156, 2267–2272 (2011). https://doi.org/10.1007/s00705-011-1094-9

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  • DOI: https://doi.org/10.1007/s00705-011-1094-9

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