Abstract
Porcine enteric caliciviruses (PEC) belong to the genera Norovirus and Sapovirus within the family Caliciviridae. They are enteric pathogens and are considered potential zoonotic agents. In this study, the circulation of PEC was evaluated by RT-PCR of stool samples and intestinal contents of pigs raised in Rio de Janeiro State, Brazil. Both porcine norovirus (PoNoV) and porcine sapovirus (PoSaV) were detected. The PoNoV strains were classified as genogroup II, genotypes 11, 18 and 19. The PoSaV strains were classified as genogroups III and VII, though some strains could not be classified into any established genogroup, potentially representing a new one. PEC were detected mainly in animals without clinical signs of gastroenteritis.
References
Barry AF, Alfieri AF, Alfieri AA (2008) High genetic diversity in RdRp gene of Brazilian porcine sapovirus strains. Vet Microbiol 131(1–2):185–191
Collins PJ, Martella V, Buonavoglia C, O’Shea H (2009) Detection and characterization of porcine sapoviruses from asymptomatic animals in Irish farms. Vet Microbiol 139:176–182
Cunha JB, Mendonça MCL, Miagostovich MP, Leite JPG (2010) First detection of porcine norovirus GII.18 in Latin America. Res Vet Sci 89:126–129
Farkas T, Nakajima S, Sugieda M, Deng X, Zhong W, Jiang X (2005) Seroprevalence of noroviruses in swine. J Clin Microbiol 43(2):657–661
Farkas T, Zhong WM, Jing Y, Huang PW, Espinosa SM, Martinez N, Morrow AL, Ruiz-Palacios GM, Pickering LK, Jiang X (2004) Genetic diversity among sapoviruses. Arch Virol 149:1309–1323
Guo M, Hayes J, Cho KO, Parwani AV, Lucas LM, Saif LJ (2001) Comparative pathogenesis of tissue culture-adapted and wild-type Cowden porcine enteric calicivirus (PEC) in gnotobiotic pigs and induction of diarrhea by intravenous inoculation of wild-type PEC. J Virol 75(19):9239–9251
Jiang X, Huang PW, Zhong WM, Farkas T, Cubitt DW, Matson DO (1999) Design and evaluation of a primer pair that detects both Norwalk- and Sapporo-like caliciviruses by RT-PCR. J Virol Methods 83:145–154
Jeong C, Park S, Park S, Kim H, Park S, Jeong J, Choy HE, Saif LJ, Kim S, Kang M, Hyun B, Cho K (2007) Genetic diversity of porcine sapoviruses. Vet Microbiol 122:246–257
L’Homme Y, Sansregret R, Plante-Fortier E, Lamontagne A, Lacroix G, Ouardani M, Deschamps J, Simard G, Simard C (2009) Genetic diversity of porcine norovirus and sapovirus: Canada, 2005–2007. Arch Virol 154(4):581–593
Keum H, Moon H, Park S, Kim H, Rho S, Park B (2009) Porcine noroviruses and sapoviruses on Korean swine farms. Arch Virol 154(11):1765–1774
Kim HJ, Cho HS, Cho KO, Park NY (2006) Detection and molecular characterization of porcine enteric calicivirus in Korea, genetically related to sapoviruses. J Vet Med B Infect Dis Vet Public Health 53:155–159
Martella V, Bányai K, Lorusso E, Bellacicco AL, Decaro N, Mari V, Saif L, Costantini V, De Grazia S, Pezzotti G, Lavazza A, Buonavoglia C (2008) Genetic heterogeneity of porcine enteric caliciviruses identified from diarrhoeic piglets. Virus Genes 36(2):365–373
Martella V, Lorusso E, Banyai K, Decaro N, Corrente M, Elia G, Cavalli A, Radogna A, Costantini V, Saif L, Lavazza A, Di Trani L, Buonavoglia C (2008) Identification of a porcine calicivirus related genetically to human sapoviruses. J Clin Microbiol 46(6):1907–1913
Martínez MA, Alcalá AC, Carruyo G, Botero L, Liprandi F, Ludert JE (2006) Molecular detection of porcine enteric caliciviruses in Venezuelan farms. Vet Microbiol 116:77–84
Mauroy A, Scipioni A, Mathijs E, Miry C, Ziant D, Thys C, Thiry E (2008) Noroviruses and sapoviruses in pigs in Belgium. Arch Virol 153(10):1927–1931
Otto TD, Vasconcellos EA, Gomes LHF, Moreira AS, Degrave WM, Mendonça-Lima L, Alves-Ferreira M (2008) ChromaPipe: a pipeline for analysis, quality control and management for a DNA sequencing facility. Genet Mol Res 7(3):861–871
Reuter G, Biro H, Szucs G (2007) Enteric caliciviruses in domestic pigs in Hungary. Arch Virol 152:611–614
Schuffenecker I, Ando T, Thouvenot D, Lina B, Aymard M (2001) Genetic classification of “Sapporo-like virus”. Arch Virol 146:2115–2132
Scipioni A, Mauroy A, Vinjé J, Thiry E (2008) Animal noroviruses. Vet J 178:32–45
Shen Q, Wen Z, Yang S, Chen Y, Ning H, Shan T, Liu J, Yang Z, Cui L, Zhu J, Hua X (2009) Molecular detection and prevalence of porcine caliciviruses in eastern China from 2008 to 2009. Arch Virol 154:1625–1630
Vinjé J, Hamidjaja RA, Sobsey MD (2004) Development and application of a capsid VP1 (region D) based reverse transcription PCR assay for genotyping of genogroup I and II noroviruses. J Virol Methods 116:109–117
Wang QH, Costantini V, Saif L (2007) Porcine enteric caliciviruses: genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology. Vaccine 25:5453–5466
Wang QH, Han MG, Cheetham S, Souza M, Funk JA, Saif LJ (2005) Porcine noroviruses related to human noroviruses. Emerg Infect Dis 11:1874–1881
Wang QH, Han MG, Funk JA, Bowman G, Janies DA, Saif LJ (2005) Genetic diversity and recombination of porcine sapoviruses. J Clin Microbiol 43(12):5963–5972
Wang QH, Souza M, Funk JA, Zhang W, Saif LJ (2006) Prevalence of noroviruses and sapoviruses in swine of various ages determined by reverse transcription-PCR and microwell hybridization assays. J Clin Microbiol 44(6):2057–2062
Wolf S, Williamson W, Hewitt J, Lin S, Rivera-Aban M, Ball A, Scholes P, Savill M, Greening GE (2009) Molecular detection of norovirus in sheep and pigs in New Zealand farms. Vet Microbiol 133(1–2):184–189
Yin Y, Tohya Y, Ogawa Y, Numazawa K, Kato K, Akashi H (2006) Genetic analysis of calicivirus genomes detected in intestinal contents of piglets in Japan. Arch Virol 151(9):1749–1759
Zhang W, Shen Q, Hua X, Cui L, Liu J, Yang S (2008) The first Chinese porcine sapovirus strain that contributed to an outbreak of gastroenteritis in piglets. J Virol 82(16):8239–8240
Zheng DP, Ando T, Fankhauser RL, Beard RS, Glass RI, Monroe SS (2006) Norovirus classification and proposed strain nomenclature. Virology 346:312–323
Acknowledgments
We thank the Genomic Platform—DNA Sequencing PDTIS/Fiocruz for technical assistance. JB Cunha was a master student of Cellular and Molecular Biology Program at Oswaldo Cruz Institute/Fiocruz and a recipient of scholarship from the Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES). This study was supported by Oswaldo Cruz Institute/Fiocruz, Carlos Chagas Filho Foundation for Research Support of the State of Rio de Janeiro (FAPERJ) and National Council for Scientific and Technological Development (CNPq).
Conflict of interest statement
The authors declare that they have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Cunha, J.B., de Mendonça, M.C.L., Miagostovich, M.P. et al. Genetic diversity of porcine enteric caliciviruses in pigs raised in Rio de Janeiro State, Brazil. Arch Virol 155, 1301–1305 (2010). https://doi.org/10.1007/s00705-010-0695-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-010-0695-z