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Genomic expression patterns in cell separation mutants of Schizosaccharomyces pombe defective in the genes sep10 + and sep15 + coding for the Mediator subunits Med31 and Med8

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Abstract

Cell division is controlled by a complex network involving regulated transcription of genes and postranslational modification of proteins. The aim of this study is to demonstrate that the Mediator complex, a general regulator of transcription, is involved in the regulation of the second phase (cell separation) of cell division of the fission yeast Schizosaccharomyces pombe. In previous studies we have found that the fission yeast cell separation genes sep10 + and sep15 + code for proteins (Med31 and Med8) associated with the Mediator complex. Here, we show by genome-wide gene expression profiling of mutants defective in these genes that both Med8 and Med31 control large, partially overlapping sets of genes scattered over the entire genome and involved in diverse biological functions. Six cell separation genes controlled by the transcription factors Sep1 and Ace2 are among the target genes. Since neither sep1 + nor ace2 + is affected in the mutant cells, we propose that the Med8 and Med31 proteins act as coactivators of the Sep1-Ace2-dependent cell separation genes. The results also indicate that the subunits of Mediator may contribute to the coordination of cellular processes by fine-tuning of the expression of larger sets of genes.

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Acknowledgments

We thank Ilona Lakatos for technical assistance. This research was supported by grants from the Hungarian National Fund for Scientific Research (OTKA T042694 and OTKA F48765), by Cancer Research UK [CUK] Grant No. C9546/A6517 (to J.B.), and a Wellcome Trust Short-Term Travel Grant to I.M.

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Authors and Affiliations

  1. Department of Genetics and Applied Microbiology, University of Debrecen, Debrecen, Hungary

    Ida Miklos, Zsolt Szilagyi, Erika Zilahi, Gyula Batta, Zsuzsa Antunovics, Klara Enczi & Matthias Sipiczki

  2. Cancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1HH, UK

    Stephen Watt & Jürg Bähler

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  1. Ida Miklos
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  2. Zsolt Szilagyi
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  4. Erika Zilahi
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  8. Jürg Bähler
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  9. Matthias Sipiczki
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Correspondence to Matthias Sipiczki.

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Communicated by S. Hohmann.

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Miklos, I., Szilagyi, Z., Watt, S. et al. Genomic expression patterns in cell separation mutants of Schizosaccharomyces pombe defective in the genes sep10 + and sep15 + coding for the Mediator subunits Med31 and Med8. Mol Genet Genomics 279, 225–238 (2008). https://doi.org/10.1007/s00438-007-0296-z

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