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The effect of milk fermented by yogurt cultures plus Lactobacillus casei DN-114001 on the immune response of subjects under academic examination stress

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Summary

Background

A suppressed immune response has been documented in students under examination stress.

Aims

The current study aimed to evaluate the effect of milk fermented with yogurt cultures plus Lactobacillus casei DN-114001 (Actimel®) on the immune system of subjects under academic examination stress.

Methods

University students were allocated to one of two groups, receiving during 6 weeks (3 weeks prior to, as well as the 3-week duration of the examination period) either: a) a glass of semi-skimmed milk each day (control group, n=63) or b) two 100mL portions per day of fermented milk (treatment group, n=73). Anxiety and immunological measurements were monitored at baseline (Phase 0) and study end (Phase 1).

Results

The results were expressed as the differences between the data obtained from Phase 0 and Phase 1. This was calculated by subtracting Phase 1 results from the Phase 0 and it is denominated “Treatment effect”. Mean (± SE) anxiety increased significantly (P<0.05) over the 6-week study in all students, from 40.74±2.50 to 61.19±2.64 (in percentiles). There was no significant treatment effect since this increase was similar in the control and the treatment groups (21.65±5.09 vs 19.14±3.67, respectively). However, there was a significant treatment effect (P<0.05) on the mean change in absolute number of lymphocytes during the 6-week study, which decreased in the control group (–0.04±0.12 cells x 103/mm3) and increased in the treatment group (0.37±0.11 cells x 103/mm3). There was also a significant treatment effect (P<0.05) on the change in absolute numbers of CD56 cells during the 6-week study. Mean absolute CD56 cells significantly decreased (P<0.05) in the control group (–51.97±21.33 cells/mm3),while remaining similar in the treatment group (17.29±17.27 cells/mm3). During the study, mean serum cortisol increased 4.30±0.98 µg/dL in the control group, and 1.75±1.05 µg/dL in the treatment group and no significant differences were found between both values (P=0.062).

Conclusions

Milk fermented with yogurt cultures plus Lactobacillus casei DN-114001 was able to modulate the number of lymphocytes and CD56 cells in subjects under academic examination stress.

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Authors and Affiliations

  1. Grupo Inmunonutrición, Depo. de Metabolismo y Nutrición, Instituto del Frío, CSIC, C/Jose Antonio Novais 10, 28040, Madrid, Spain

    Ascensión Marcos PhD, Julia Wärnberg BSc, Esther Nova PhD, Sonia Gómez PhD & Rocío Alvarez BSc

  2. Unidad de Citometría Centro Nacional de Investigaciones Cardiovasculares, Instituto de Salud Carlos III, Madrid, Spain

    Alberto Alvarez PhD

  3. Coordinación Científica, Danone S. A., Barcelona, Spain

    José A. Mateos PhD & José M. Cobo PhD

Authors
  1. Ascensión Marcos PhD
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  2. Julia Wärnberg BSc
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  3. Esther Nova PhD
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  4. Sonia Gómez PhD
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Correspondence to Ascensión Marcos PhD.

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Marcos, A., Wärnberg, J., Nova, E. et al. The effect of milk fermented by yogurt cultures plus Lactobacillus casei DN-114001 on the immune response of subjects under academic examination stress. Eur J Nutr 43, 381–389 (2004). https://doi.org/10.1007/s00394-004-0517-8

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  • DOI: https://doi.org/10.1007/s00394-004-0517-8

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