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MicroRNA expression profiles in conventional and micropropagated strawberry (Fragaria × ananassa Duch.) plants

  • Cell Biology and Morphogenesis
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Abstract

MicroRNAs (miRNAs) are a class of small non-coding RNAs which play a critical role in plant growth and development. To detect strawberry miRNAs and discover the expression difference between conventional and micropropagated strawberry plants, we carried out the detection and quantification of strawberry miRNAs by microarray. The main findings were that 74 miRNAs were checked in strawberry plants and four miRNA genes displayed clear expression difference between conventional and micropropagated strawberry plants, including two up-regulated genes (miR535 and miR390) and two down-regulated genes (miR169a and miR169d). The ratios of conventionally propagated strawberry plant/micropropagated strawberry plant for miR535, miR390, miR169a and miR169d were 2.6884, 2.2673, 0.2496 and 0.3814, respectively. Quantitative reverse transcription polymerase chain reaction applied to the two up-regulated genes (miR535 and miR390) validated the microarray result. This is the first report on differential expression of miRNAs in conventional and micropropagated plants.

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Abbreviations

IBA:

Indole-3-butyric acid

RT:

Reverse transcription

PCR:

Polymerase chain reaction

RT-PCR:

Reverse transcription-polymerase chain reaction

Q-RT-PCR:

Quantitative-reverse transcription-polymerase chain reaction

cDNA:

Complementary deoxyribonucleic acid

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Acknowledgments

This work was financially supported by National Natural Science Foundation of China (30671432) and Program for New Century Excellent Talents in University (NCET-07-0565).

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  1. College of Horticulture, Shenyang Agricultural University, Dongling Road 120, 110161, Shenyang, Liaoning, People’s Republic of China

    He Li, Zhihong Zhang, Feifei Huang, Linlin Chang & Yue Ma

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  1. He Li
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  2. Zhihong Zhang
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Correspondence to Zhihong Zhang.

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Communicated by R. Reski.

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Li, H., Zhang, Z., Huang, F. et al. MicroRNA expression profiles in conventional and micropropagated strawberry (Fragaria × ananassa Duch.) plants. Plant Cell Rep 28, 891–902 (2009). https://doi.org/10.1007/s00299-009-0693-3

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