Skip to main content

Advertisement

Springer Nature Link
Log in

Fermented soy product supplemented with isoflavones affects adipose tissue in a regional-specific manner and improves HDL-cholesterol in rats fed on a cholesterol-enriched diet

  • Original paper
  • Published:
European Food Research and Technology Aims and scope Submit manuscript

Abstract

It has been proposed that isoflavones and probiotics possess specific biological activities that enable them to affect lipid metabolism. The purpose of this study was to evaluate the effects of a soy product fermented with Enterococcus faecium and Lactobacillus jugurti and supplemented with isoflavones on the plasma lipid and glucose profiles, and on the retroperitoneal (RET) and epididymal (EPI) adipose tissue adipocyte areas in juvenile rats. Male Wistar rats were subjected to 6-week experimental diets: (1) cholesterol-enriched diet (rat chow) plus fermented soy product supplemented with isoflavones, (2) cholesterol-enriched diet plus unfermented placebo, (3) cholesterol-enriched diet plus placebo supplemented with isoflavones, (4) standard diet (rat chow), or (5) cholesterol-enriched diet. Soy products affected adipose tissue in a regional-specific manner and may be responsible for the observed increase in RET adipocyte area (μ2): (1) 12757.00 ± 286.25; (2) 13269.00 ± 326.87 or (3) 13050.00 ± 226.85 versus (4) 8714.92 ± 262.41 or (5) 8892.70 ± 131.79 or decrease in EPI adipocyte area (μ2): (1) 7759.00 ± 108.89 and (2) 7481.90 ± 111.21 compared with the control group (4) 8346.60 ± 158.89. The effect on epididymal adipose tissue was probably due to isoflavones. Probiotics significantly raised the plasma level of HDL cholesterol. Fermented soy product supplemented with isoflavones did not promote changes in the glucose and triglyceride plasma levels. This product may offer a new approach for improving or preventing lipid metabolism abnormalities by decreasing visceral adipocyte area, adjusting cholesterol-mediated loss of RET adipose tissue (lipoatrophy), and increasing the HDL cholesterol plasma level.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Canada)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Abd El-Gawad IA, El-Sayed EM, Hafez SA, El-Zeini HM, Saleh FA (2005) Int Dairy J 15:37–44

    Article  CAS  Google Scholar 

  2. Williams RR, Hopkins PN, Hunt SC, Wu LL, Hasstedt SJ, Lalouel JM, Ash KO, Stults BM, Kuida H (1990) Arch Intern Med 150:582–588

    Article  CAS  Google Scholar 

  3. Gordon DJ, Jl Probstfield, Garrison RJ (1989) Circulation 79:8–15

    CAS  Google Scholar 

  4. Castelli WP, Garrison RJ, Wilson PWF, Abbott RD, Kalousdian S, Kannel WB (1986) J Am Med Assoc 256:2835–2838

    Article  CAS  Google Scholar 

  5. Lee YK, Salminem S (1995) Trends Food Sci Technol 6:241–245

    Article  Google Scholar 

  6. Fuller R (1989) J Appl Bacteriol 66:365–378

    CAS  Google Scholar 

  7. Scheinbach S (1998) Biotechnol Adv 16(3):581–608

    Article  CAS  Google Scholar 

  8. Liong MT (2007) Nutr Rev 65(7):316–328

    Article  Google Scholar 

  9. Simons LA, Amansec SG, Conway P (2006) Nutr Metab Cardiovasc Dis 16(8):531–535

    Article  Google Scholar 

  10. Ali AA, Velasquez MT, Hansen CT, Mohamed SJ, Bathena SJ (2004) J Nutr Biochem 15:583–590

    Article  CAS  Google Scholar 

  11. Pereira DI, Gibson GR (2002) Crit Rev Biochem Mol Biol 37(4):259–281

    Article  CAS  Google Scholar 

  12. Krause BR, Hartman AD (1984) J Lipid Res 25:97–110

    CAS  Google Scholar 

  13. Farkas J, Angel A, Avigan MI (1973) J Lipid Res 14:344–356

    CAS  Google Scholar 

  14. Quintão E, Grundy SM, Ahrens EH (1971) J Lipid Res 12:233–247

    Google Scholar 

  15. Islam KK, Knight BL, Frayn KN, Patel DD, Gibbons GF (2005) Biochim Biophys Acta 1734(3):259–268

    CAS  Google Scholar 

  16. Farmakalidis E, Hathcock JN, Murphy PA (1985) Food Chem Toxicol 23:741–745

    Article  CAS  Google Scholar 

  17. Nazz A, Yellayi S, Zakroczymski M, Bunick D, Doerge DR, Lubahn DB, Helferich WG, Cooke PS (2003) The soy isoflavone genistein decreases adipose deposition in mice. Endocrinology 144(8):3315–3320

    Article  Google Scholar 

  18. Dieudonne MN, Leneveu MC, Giudicelli Y, Pecquery R (2004) Am J Physiol Cell Physiol 286(3):C655–661

    Article  CAS  Google Scholar 

  19. Manzoni MSJ, Rossi EA, Carlos IZ, Vendramini RC, Duarte ACGO, Dâmaso AR (2005) Nutrition 21:1018–1024

    Article  CAS  Google Scholar 

  20. National Research Council (1985) Guide for care and use of laboratory animals. National Academy of Sciences, Washington

    Google Scholar 

  21. Rossi EA, Vendramini RC, Carlos IZ, Ueiji IS, Squinzari MM, Silva SI Jr, Valdez GF (2000) Arq Bras Cardiol 74(3):213–216

    Article  Google Scholar 

  22. Rossi EA, Rosier I, Damaso AR, Carlos IZ, Vendramini RC, Abdala DSP, Talarico VH, Minto DF (2004) Aliment Nutr 15(2):93–99

    CAS  Google Scholar 

  23. Rossi EA, Vendramini RC, Carlos IZ, de Oliveira MG, de Valdez GF (2003) Arch Latinoam Nutr 53(1):47–51

    CAS  Google Scholar 

  24. Hirsch J, Gallian E J (1968) Lipid Res 9:110–119

    CAS  Google Scholar 

  25. Tchkonia T, Lenburg M, Thomou T, Giorgadze M, Frampton G, Pirtskhalava T, Cartwright A, Cartwright M, Flanagam J, Karagiannides I, Gerry N, Forse RA, Tchoukalova Y, Jensen MD, Pothoulakis C, Kirkland JL (2007) Am J Physiol Endocrinol Metab 292(1):E298–E307

    Article  CAS  Google Scholar 

  26. Tchkonia T, Giorgadze M, Pirtskhalava T, Thomou T, DePonte M, Koo Forse RA, Chinnappan D, Martin-Ruiz C, von Zglinicki T, Kirkland JL (2006) Diabetes 55(9):2571–2578

    Article  CAS  Google Scholar 

  27. Pedersen SB, Borglum JD, Eriksen EF, Richelsen B (1991) Biochim Biophys Acta 1093(1):80–86

    Article  CAS  Google Scholar 

  28. Cooke PS, Heine PA, Taylor JA, Lubahn DB (2001) Mol Cell Endocrinol 178:147–154

    Article  CAS  Google Scholar 

  29. Haffner SM (2007) Am J Med 120 (9 Suppl 1):S10–S17

  30. Giorgino F, Laviola L, Eriksson JW (2005) Acta Physiol Scand 183(1):13–30

    Article  CAS  Google Scholar 

  31. Frayn KN (2002) Diabetologia 45:1201–1210

    Article  CAS  Google Scholar 

  32. Nagasawa A, Fukui K, Kojima M, Kishida K, Maeda N, Nagaretani H, Hibuse T, Nishizawa H, Kihara S, Waki M, Takamatsu K, Funahashi T, Matsuzawa Y (2003) Biochem Biophys Res Commun 311:909–914

    Article  CAS  Google Scholar 

  33. Chen JR, Chiou SF, Suetsuna K, Yang HY, Yang SC (2003) Nutrition 19:676–680

    Article  CAS  Google Scholar 

  34. Zulet MA, Barber A, Garcin H, Higueret P, Martínez JF (1999) J Am Coll Nutr 18(1):36–42

    CAS  Google Scholar 

  35. Feoli AM, Roehrig C, Rotta LN, Kruger AH, Souza KB, Kessler AM, Renz SV, Brusque AM, Souza DO, Perry ML (2003) Nutrition 19:789–793

    Article  CAS  Google Scholar 

  36. Calleja L, Trallero MC, Carrizona C, Méndez MT, Palacios-Alaiz E, Osada J (2000) Atherosclerosis 152:69–78

    Article  CAS  Google Scholar 

  37. Agerholm-Larsen L, Raben A, Haulrik N, Hansen AS, Manders M, Astrup A (2000) Eur J Clin Nutr 54(4):288–297

    Article  CAS  Google Scholar 

  38. Agerholm-Larsen L, Bell ML, Grunwald GK, Astrup A (2000) The effect of a probiotic milk product on plasma cholesterol: a meta-analysis of short-term interventions studies. Eur J Clin Nutr 54(11):856–860

    Article  CAS  Google Scholar 

  39. Hlivak P, Odraska J, Ferencik M, Ebringer L, Jahnova E, Mikes Z (2005) Bratisl Lek Listy 106(2):67–72

    CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior and Conselho Nacional de Desenvolvimento Científico e Tecnológico.

Author information

Authors and Affiliations

  1. Departamento de Alimentos e Nutrição, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista Júlio de Mesquita Filho, Rodovia Araraquara Jaú, Km 1, Campus Universitário, Caixa Postal 502, CEP 14802901, Araraquara, Sao Paulo, Brazil

    Marla S. Jovenasso Manzoni & Elizeu A. Rossi

  2. Departamento de Análises Clínicas, Universidade Estadual Paulista, Araraquara, Sao Paulo, Brazil

    Iracilda Z. Carlos & Regina C. Vendramini

  3. Departamento de Educação Física e Motricidade Humana, Universidade Federal de São Carlos, Sao Carlos, Sao Paulo, Brazil

    Ana C. G. O. Duarte, Neuli M. Tenório & Danielle B. Amorim

  4. Departamento de Biociências, Campus Baixada Santista, Universidade Federal de São Paulo, Santos, Sao Paulo, Brazil

    Ana R. Dâmaso

Authors
  1. Marla S. Jovenasso Manzoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Elizeu A. Rossi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Iracilda Z. Carlos
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Regina C. Vendramini
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ana C. G. O. Duarte
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Neuli M. Tenório
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Danielle B. Amorim
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ana R. Dâmaso
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marla S. Jovenasso Manzoni.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jovenasso Manzoni, M.S., Rossi, E.A., Carlos, I.Z. et al. Fermented soy product supplemented with isoflavones affects adipose tissue in a regional-specific manner and improves HDL-cholesterol in rats fed on a cholesterol-enriched diet. Eur Food Res Technol 227, 1591–1597 (2008). https://doi.org/10.1007/s00217-008-0883-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00217-008-0883-1

Keywords

Search

Navigation