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Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots

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Lipids

Abstract

Separation of polar lipids by two-dimensional thin layer chromatography providing resolution of all the lipid classes commonly encountered in animal cells and a sensitive, rapid, reproducible procedure for determination of phospholipids by phosphorus analysis of spots are described. Values obtained for brain and mitochondrial inner membrane phospholipids are presented.

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References

  1. Rouser, G., A. N. Siakotos and S. Fleischer, Lipids 1:85–86 (1966).

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Authors and Affiliations

  1. Division of Neurosciences, City of Hope Medical Center, 91010, Duarte, California

    George Rouser

  2. Department of Molecular Biology, Vanderbilt University, 37203, Nashville, Tennessee

    Sidney Fleischer

  3. City of Hope Medical Center, 91010, Duarte, California

    Akira Yamamoto

Authors
  1. George Rouser
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  2. Sidney Fleischer
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  3. Akira Yamamoto
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Cite this article

Rouser, G., Fleischer, S. & Yamamoto, A. Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids 5, 494–496 (1970). https://doi.org/10.1007/BF02531316

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  • DOI: https://doi.org/10.1007/BF02531316

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