Abstract
The existence of six dsRNA segments ofTrichomonas vaginalis virus was confirmed and the molar mass and relative abundance of these segments were determined by agarose gel electrophoresis with reovirus dsRNA serving as a standard. TheM’s were 3.5, 3.4, 3.2, 2.5, 1.4 and 0.34 Mg/mol for the two strains studied, the relative abundances, however, were 1.0, 1.4, 3.0, 0.3, 2.7, 4.2 and 1.0, 0.6, 1.7, 0.5, 3.4 1.0 for these strains, respectively. Cell homogenate fractionation showed that all dsRNA segments were associated with viral particles. The data appeared to support the hypothesis of a relationship between viruses of the protozoanT. vaginalis and of the yeastSaccharomyces cerevisiae.
Similar content being viewed by others
References
Alderete J.F., Kasmala L., Metcalfe E., Garza G.E.: Phenotypic variation and diversity amongTrichomonas vaginalis and correlation of phenotyoe with trichomonal virulence determinants.Infect. Immun. 53, 285–293 (1986).
Alderete J.F., Garza G.E.: Specific nature ofTrichomonas vaginalis parasitism of host cell surfaces.Infect. Immun. 50, 701–708 (1985).
Čerkasovová A., Novák J., Čerkasov J., Kulda J., Tachezy J.: Metabolic properties ofTrichomonas vaginalis resistant to metronidazole under anaerobic conditions, inTrichomonads and Trichomoniasis, Part 2 (J. Kulda, J. Čerkasov, eds.).Acta Univ. Carolinae Biol. 30, 505–512 (1986).
Diamond L.S.: The establishment of various trichomonads of animals and man in axenic cultures.J. Parasitol. 43, 488–490 (1957).
Flegr J., Čerkasov J., Kulda J., Tachezy J., Štokrová J.: The dsRNA ofTrichomonas vaginalis is associated with virus-like particles and does not correlate with metronidazole resistance.Folia Microbiol. 32, 345–348 (1987a).
Flegr J., Čerkasov J., Čerkasovová A., Kulda J., Štokrová J.: Double stranded RNA inTrichomonas vaginalis, inTrichomonads and Trichomoniasis, Part I (J. Kulda, J. Čerkasov, eds.).Acta Univ. Carolinae Biol. 30, 281–286 (1987b).
Flegr J.: A rapid method for isolation of double stranded RNA.Prepar. Biochem. 17, 423–433 (1987).
Frank H., Schwarz H., Graf T., Schafer W.: Properties of mouse leukemia viruses. XV. Electron microscopic studies on the organisation of Friend leukemia virus and other mammalian C-type viruses.Z. Naturforsch. 33e, 124–128 (1978).
Edmanndson S.P., Gray D.M.: Analysis of electrophoretic properties of double stranded DNA and RNA in agarose gels at a finite voltage gradient.Biopolymers 23, 2725–2742 (1984).
Maniatis T., Fritsch E.F., Sambrook J.:A Laboratory Manual, Molecular Cloning, Cold Spring Harbor Laboratory: Cold Spring Harbor, New York 1983.
Meingassner J.G., Thurner J.: Strain ofTrichomonas vaginalis resistant to metronidazole and other 5-nitroimidazoles.Antimicrob. Agents Chemother. 15, 254–257 (1979).
Tipper D.J., Bostian K.A.: Double stranded ribonucleic acid killer systems in yeasts.Microbiol. Rev. 48, 125–156 (1984).
Wang A.L., Wang C.C.: A linear double stranded RNA inTrichomonas vaginalis.J. Biol. Chem. 260, 3697–3702 (1985).
Wang A.L., Wang C.C.: The double stranded RNA inTrichomonas vaginalis may originate from virus-like particles.Proc. Nat. Aoad. Sci. USA 83, 7956–7961 (1986).
Wang A.L., Wang C.C., Alderets J.F.:Trichomonas vaginalis phenotypic variation occurs only among trichomonads infected with the double-stranded RNA virus.J. Exp. Med. 166, 142–150 (1987).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Flegr, J., Čerkasov, J. & Štokrová, J. Multiple populations of double-stranded RNA in two virus-harbouring strains ofTrichomonas vaginalis . Folia Microbiol 33, 462–465 (1988). https://doi.org/10.1007/BF02925772
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02925772