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Thyrotropin-releasing hormone increases the levels of c-fos and β-actin mRNA in GH3/B6 pituitary tumor cells

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Summary

The effects of thyrotropin-releasing hormone (TRH) on the mRNA levels of c-fos, N-myc, β-actin and prolactin (PRL) were studied in GH3/B6 cells, a rat pituitary cell line. TRH has previously been shown to increase biosynthesis and release of PRL, and to stimulate PRL gene transcription (13,19) in these cells. All experiments were performed on quiescent serum-deprived cells, and under these conditions, addition of TRH stimulated PRL production but did not alter cellular proliferation. Simultaneously, TRH induced time- and concentration-dependent increases in c-fos and β-actin mRNA levels, with peak responses at 30 min and 4 h, respectively, in a dose range from 1 nM to 100 nM. The TRH effects on N-myc mRNA levels were less consistent. Addition of serum to quiescent GH3/B6 cells induced (3–5 fold) increases in c-fos, β-actin and PRL mRNAs which differed in magnitude and kinetics when compared to TRH stimulation. Serum did not alter N-myc mRNA levels. The TRH-induced increases in c-fos and β-actin mRNA may play a role in the secretory response.

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Authors and Affiliations

  1. Groupe de Neuroendocrinologie Cellulaire et Moleculaire, U.A. C.N.R.S. 1115, Collège de France, 11 Place Marcelin Berthelot 75231, Paris Cedex 05, France

    Andrea S. Weisman, Andrée Tixier-Vidal & Danielle Gourdji

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  1. Andrea S. Weisman
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  2. Andrée Tixier-Vidal
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  3. Danielle Gourdji
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Dr. Weisman is supported by a CNRS fellowship for visiting foreign scientists.

This work was supported by the Centre National de la Recherche Scientifique, UA 1115.

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Weisman, A.S., Tixier-Vidal, A. & Gourdji, D. Thyrotropin-releasing hormone increases the levels of c-fos and β-actin mRNA in GH3/B6 pituitary tumor cells. In Vitro Cell Dev Biol 23, 585–590 (1987). https://doi.org/10.1007/BF02620977

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