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Cell culture for three-dimensional modeling in rotating-wall vessels: An application of simulated microgravity

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Journal of tissue culture methods

Summary

High-density, three-dimensional cell cultures are difficult to grow in vitro. The rotating-wall vessel (RWV) described here has cultured BHK-21 cells to a density of 1.1 × 107 cells/ml. Cells on microcarriers were observed to grow with enhanced bridging in this batch culture system. The RWV is a horizontally rotated tissue culture vessel with silicon membrane oxygenation. This design results in a low-turbulence, low-shear cell culture environment with abundant oxygenation. The RWV has the potential to culture a wide variety of normal and neoplastic cells.

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Authors and Affiliations

  1. KRUG Life Sciences, 1290 Hercules Drive, Suite 120, 77058, Houston, Texas

    Ray P. Schwarz & Thomas J. Goodwin

  2. NASA, Johnson Space Center, Houston, Texas

    David A. Wolf

Authors
  1. Ray P. Schwarz
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  2. Thomas J. Goodwin
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  3. David A. Wolf
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Schwarz, R.P., Goodwin, T.J. & Wolf, D.A. Cell culture for three-dimensional modeling in rotating-wall vessels: An application of simulated microgravity. Journal of Tissue Culture Methods 14, 51–57 (1992). https://doi.org/10.1007/BF01404744

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  • DOI: https://doi.org/10.1007/BF01404744

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