Summary
Protoplasts of methionine-and lysine-requiring h- mutants isolated from the L972 h- strain of Schizosaccharomyces pombe were fused. The protoplasts were obtained from the cells with enzymes produced by Trichoderma viride. When a mixture of the protoplasts was treated with 30% PEG 4000 solution containing 10 mM CaCl2, cell fusion and complementation was attained with a frequency of 0.17%. Both fusion partners were recovered among the spores after crossing of the fusion products with the strain M210 ade6 h+. Cytological and haploidization examinations showed that the fusion cells are not heterokaryons, and that the increased amount of genetic material is situated in one nucleus.
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Communicated by F. Kaudewitz
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Sipiczki, M., Ferenczy, L. Protoplast fusion of Schizosaccharomyces pombe auxotrophic mutants of identical mating-type. Molec. Gen. Genet. 151, 77–81 (1977). https://doi.org/10.1007/BF00446915
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DOI: https://doi.org/10.1007/BF00446915