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Preliminary characterization of calcium binding in islet-cell plasma membranes

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  • Published: November 1980
  • Volume 19, pages 439–444, (1980)
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Diabetologia Aims and scope Submit manuscript
Preliminary characterization of calcium binding in islet-cell plasma membranes
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  • S. P. Naber1,2,
  • J. M. McDonald1,
  • L. Jarett1,
  • M. L. McDaniel1,
  • C. W. Ludvigsen1 &
  • …
  • P. E. Lacy1 

  • 215 Accesses

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Summary

Preliminary characterization of calcium binding was determined in a highly-enriched islet-cell plasma membrane fraction using a membrane filtration technique. Equilibrium calcium binding was specific, concentration dependent and saturable. Scatchard analysis indicated the existence of more than one class of calcium binding sites. The affinity constants and maximum binding capacities were 1.14 ×105 M-1 and 1.2 picomol/μg protein and 1.17× 103 M-1 and 64.8 picomol/μg for the high and low affinity sites, respectively. Bound 45Ca2+ was dissociated from the plasma membranes in a biphasic manner in the presence of excess unlabelled calcium.

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Authors and Affiliations

  1. Department of Pathology, Washington University School of Medicine, St. Louis, Missouri, USA

    S. P. Naber, J. M. McDonald, L. Jarett, M. L. McDaniel, C. W. Ludvigsen & P. E. Lacy

  2. Elliot P. Joslin Research Laboratory, Harvard Medical School, One Joslin Place, 02215, Boston, MA, USA

    S. P. Naber

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  1. S. P. Naber
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  2. J. M. McDonald
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  4. M. L. McDaniel
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  5. C. W. Ludvigsen
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Naber, S.P., McDonald, J.M., Jarett, L. et al. Preliminary characterization of calcium binding in islet-cell plasma membranes. Diabetologia 19, 439–444 (1980). https://doi.org/10.1007/BF00281823

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  • Received: 27 May 1980

  • Issue Date: November 1980

  • DOI: https://doi.org/10.1007/BF00281823

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Key words

  • Islets of Langerhans
  • plasma membrane
  • calcium binding
  • 45calcium
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