Skip to main content
Springer Nature Link
Log in

Human Hematopoiesis in SCID Mice

  • Chapter
Human Hematopoiesis in SCID Mice

Part of the book series: Medical Intelligence Unit ((MIU.LANDES))

  • 26 Accesses

Abstract

The hematopoietic system is organized as a hierarchy where the majority of the cells are mature, and therefore, need to be continuously replenished from a small pool of immature progenitors. Ultimately, the entire hematopoietic system is derived from stem cells that have extensive proliferative and differentiation capacity and give rise to all myeloid and lymphoid lineages. Insight into the biology of these stem cells is, therefore, fundamental to our understanding of the regulation of normal and abnormal hematopoiesis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Chapter
USD 29.95
Price excludes VAT (Canada)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (Canada)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (Canada)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  1. Metcalf D. Haemopoietic colonies: In vitro cloning of normal and leukemic cells. Recent Results in Cancer Res 1977; 61: 1–227.

    Google Scholar 

  2. Fauser A, Messner H. Granuloerythropoietic colonies in human marrow, peripheral blood and cord blood. Blood 1978; 52: 1243–48.

    PubMed  CAS  Google Scholar 

  3. Leary A, Ogawa M. Blast colony assay for umbilical blood and adult bone marrow progenitors. Blood 1987; 69: 953–56.

    PubMed  CAS  Google Scholar 

  4. Dexter TM, Moore MAS, Sheridan APC. Maintenance of hematopoietic stem cells and production of differentiated progeny in allogeneic and semiallogenic bone marrow chimeras in vitro. J Exp Med 1977; 145: 1612–16.

    Article  PubMed  CAS  Google Scholar 

  5. Fraser CC, Szilvassy SJ, Eaves CJ et al. Proliferation of totipotent hematopoietic stem cells in vitro with retention of long-term competitive in vivo reconstituting ability. Proc Natl Acad Sci USA 1992; 89: 1968–72.

    Article  PubMed  CAS  Google Scholar 

  6. van der Sluijs JP, van den Bos C, Baert MRM et al. Loss of long-term repopulating ability in long-term bone marrow culture. Leukemia 1993; 7: 725–32.

    PubMed  Google Scholar 

  7. Phillips RA. Hematopoietic stem cells: concepts, assays, and controversies. Sem Immunol 1991; 3: 337–47.

    CAS  Google Scholar 

  8. Till JE, McCulloch EA. Hemopoietic stem cell differentiation. Biochim Biophys Acta 1980; 605: 431–59.

    PubMed  CAS  Google Scholar 

  9. Till JE, McCulloch EA. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat Res 1961; 14: 213–22.

    Article  PubMed  CAS  Google Scholar 

  10. Paige CJ, Kincade PW, Shinefeld LA et al. Precursors of murine B lymphocytes: Physical and functional characterization, and distinctions from myeloid stem cells. J Exp Med 1981; 153: 154–65.

    Article  PubMed  CAS  Google Scholar 

  11. Lemischka IR. Clonal, in vivo behavior of the totipotent hematopoietic stem cell. Sem Immunol 1991; 3: 349–55.

    CAS  Google Scholar 

  12. Abramson S, Miller RG, Phillips RA. The identification in adult bone marrow of pluripotent and restricted stem cells of the myeloid and lymphoid systems. J Exp Med 1977; 145: 1567–79.

    Article  PubMed  CAS  Google Scholar 

  13. Dick JE, Magli MC, Huszar D et al. Introduction of a selectable gene into primitive stem cells capable of long-term reconstitution of the hemopoietic system of W/LW mice. Cell 1985; 42: 71–9.

    Article  PubMed  CAS  Google Scholar 

  14. Keller G, Paige C, Gilboa E et al. Expression of a foreign gene in myeloid and lymphoid cells derived from multipotent hemopoietic precursors. Nature 1985; 318: 149–54.

    Article  PubMed  CAS  Google Scholar 

  15. Lemischka IR, Raulet DH, and Mulligan RC. Developmental potential and dynamic behavior of hematopoietic stem cells. Cell 1986; 45: 917–27.

    Article  PubMed  CAS  Google Scholar 

  16. Mosier D, Gulizia R, Baird S et al. Transfer of a functional human immune system to mice with severe combined immunodeficiency. Nature 1988; 335: 256–59.

    Article  PubMed  CAS  Google Scholar 

  17. Lubin I, Faktorowich Y, Lapidot T et al. Engraftment and development of human T and B cells in mice after bone marrow transplantation. Science 1991; 252: 345–76.

    Article  Google Scholar 

  18. McCune J, Namikawa R, Kaneshima H et al. The SCID-hu mouse: murine model for the analysis of human hematolymphoid differentiation and function. Science 1988; 241: 1632–39.

    Article  PubMed  CAS  Google Scholar 

  19. Kamel-Reid S, Dick JE. Engraftment of immune-deficient mice with human hematopoietic stem cells. Science 1988; 242: 1706–09.

    Article  PubMed  CAS  Google Scholar 

  20. Kaneshima H, Namikawa R, McCune JM. Human hematolymphoid cells in SCID mice. Curr Opin Immunol 1994; 6: 327–33.

    Article  PubMed  CAS  Google Scholar 

  21. Sutherland H, Eaves C, Lansdorp P et al. Differential regulation of primitive human hematopoietic cells in long-term cultures maintained on ge-

    Google Scholar 

  22. netically engineered murine stromal cells. Blood 1991; 78: 666–72.

    Google Scholar 

  23. Andriole G, Mule J, Hansen C et al. Evidence that lymphokine-activated killer cells and natural killer cells are distinct based on an analysis of congenitally immunodeficient mice. J Immunol 1985; 135: 2911–13.

    PubMed  CAS  Google Scholar 

  24. Rawlins DJ, Saffran DC, Tsukada S et al. Mutation of unique region of Bruton’s tyrosine kinase in immunodeficient XID mice. Science 1993; 261: 358–61.

    Article  Google Scholar 

  25. Bosma GC, Custer RP, Bosma MJ. A severe combined immunodeficiency mutation in the mouse. Nature 1983; 301: 527–30.

    Article  PubMed  CAS  Google Scholar 

  26. Fulop G, Phillips R. The scid mutation in mice causes a general defect in radiation repair. Nature 1990; 347: 479–82.

    Article  PubMed  CAS  Google Scholar 

  27. Lapidot T, Pflumio F, Doedens M et al. Cytokine stimulation of multilineage human hematopoiesis from immature cells transplanted into scid mice. Science 1992; 255: 1137–41.

    Article  PubMed  CAS  Google Scholar 

  28. Broxmeyer HE, Kurtzberg J, Gluckman E et al. Umbilical cord blood hematopoietic stem and repopulating cells in human clinical transplantation. Blood Cells 1991; 17: 313–29.

    PubMed  CAS  Google Scholar 

  29. Broxmeyer HE, Douglas GW, Hangoc G et al. Human umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells. Proc Nati Acad Sci USA 1989; 86: 3828–32.

    Article  CAS  Google Scholar 

  30. Broxmeyer HE, Hangoc G, Cooper S et al. Growth characteristics and expansion of human umbilical cord blood and estimation of its potential for transplantation in adults. Proc Natl Acad Sci USA 1992; 89: 4109–13.

    Article  PubMed  CAS  Google Scholar 

  31. Carow CE, Hangoc G, Broxmeyer HE. Human multipotential progenitor cells (CFU-GEMM) have extensive replating capacity for secondary CFUGEMM: an effect enhanced by cord blood plasma. Blood 1993; 81: 942–49.

    PubMed  CAS  Google Scholar 

  32. Lansdorp PM, Dragowska W, Mayani H. Ontogeny-related changes in proliferative potential of human hematopoietic cells. J Exp Med 1993; 178: 787–91.

    Article  PubMed  CAS  Google Scholar 

  33. Hows JM, Bradley BA, Marsh JC et al. Growth of human umbilical-cord blood in long-term hemopoietic cultures. Lancet 1992; 340: 73–6.

    Article  PubMed  CAS  Google Scholar 

  34. Vormoor J, Lapidot T, Pflumio F et al. Immature human cord blood progenitors engraft and proliferate to high levels in severe combined immunodeficient mice. Blood 1994; 83: 2489–97.

    PubMed  CAS  Google Scholar 

  35. Civin CI, Trischmann TM, Fackler MJ et al. Report on the CD34 cluster workshop. In: Knapp W, Dorken B, Gilks WR et al, eds. Leukocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press, 1990: 818.

    Google Scholar 

  36. Craig W, Kay R, Cutler RL et al. Expression of Thy-1 on human hematopoietic progenitor cells. J Exp Med 1993; 177: 1331–42.

    Article  PubMed  CAS  Google Scholar 

  37. Baum CM, Weissman IL, Tsukamoto AS et al. Isolation of a candidate human hematopoietic stem-cell population. Proc Natl Acad Sci USA 1992; 89: 2804–08.

    Article  PubMed  CAS  Google Scholar 

  38. Shultz L, Schweitzer P, Christianson S, Gott B, et al. Multiple defects in innate and adaptive immunologic function in NOD/LtSz-scid mice. J Immunol 1995; 154: 180–191.

    PubMed  CAS  Google Scholar 

  39. Lim B, Apperley JF, Orkin SH et al. Long-term expression of human adenosine deaminase in mice transplanted with retrovirus-infected hematopoietic stem cells. Proc Natl Acad Sci USA 1989; 86: 8892–96.

    Article  PubMed  CAS  Google Scholar 

  40. Bodine DM, Karlsson S, Nienhuis AW. Combination of interleukins 3 and 6 preserves stem cell function in culture and enhances retrovirusmediated gene transfer into hematopoietic stem cells. Proc Natl Acad Sci USA 1989; 86: 8897–8901.

    Article  PubMed  CAS  Google Scholar 

  41. Carter R, Abrams-Ogg A, Dick J et al. Autologous transplantation of canine long-term marrow culture cells genetically marked by retroviral vectors. Blood 1992; 79: 356–64.

    PubMed  CAS  Google Scholar 

  42. van Beusechem VW, Kukler A, Heidt PJ et al. Long-term expression of human adenosine deaminase in rhesus monkeys transplanted with retrovirus-infected bone-marrow cells. Proc Natl Acad Sci USA 1992; 89: 7640–44.

    Article  PubMed  Google Scholar 

  43. Bodine DM, Moritz T, Donahue RE et al. Long-term in vivo expression of a murine adenosine deaminase gene in rhesus monkey hematopoietic cells of multiple lineages after retroviral mediated gene transfer into CD34’ bone marrow cells. Blood 1993; 82: 1975–80.

    PubMed  CAS  Google Scholar 

  44. Dick J, Kamel-Reid S, Murdoch B et al. Gene transfer into normal human hematopoietic cells using in vitro and in vivo assays. Blood 1991; 78: 624–34.

    PubMed  CAS  Google Scholar 

  45. Ferrari G, Rossini S, Giavazzi R et al. An in vivo model of somatic cell gene therapy for human severe combined immunodeficiency. Science 1991; 251: 1363–66.

    Article  PubMed  CAS  Google Scholar 

  46. Nolta JA, Hanley MB, Kohn DB. Sustained human hematopoiesis in immunodeficient mice by cotransplantation of marrow stroma expressing human interleukin-3: Analysis of gene transduction of long-lived progenitors. Blood 1994; 83: 3041–51.

    PubMed  CAS  Google Scholar 

  47. Moritz T, Keller DC, Williams DA. Human cord blood cells as targets for gene transfer: Potential use in genetic therapy of severe combined immunodeficiency disease. J Exp Med 1993; 178: 529–36.

    Article  PubMed  CAS  Google Scholar 

  48. Karlsson S. Treatment of genetic defects in hematopoietic cell function by gene transfer. Blood 1991; 78: 2481–92.

    PubMed  CAS  Google Scholar 

  49. Weatherall DJ, Clegg JB. The Thalassaemia Syndromes. Oxford: Blackwell Scientific Publications, 1981.

    Google Scholar 

  50. Bunn HF, Forget BG. Hemoglobin: Molecular, Genetic and Clinical Aspects. Philadelphia: Saunders, 1986.

    Google Scholar 

  51. Larochelle A, Vormoor J, Lapidot T et al. Engraftment of immune-deficient mice with primitive hematopoietic cells from (3–thalassemia and sickle cell anemia patients: implications for evaluating human gene therapy protocols. Human Molecular Genetics 1995; 4: 163–172.

    Article  PubMed  CAS  Google Scholar 

  52. Daley GQ, Van Etten RA, Baltimore D. Induction of the chronic myelogenous leukemia in mice by the p2106“i”“ gene of the Philadelphia chromosome. Science 1990; 247: 824–30.

    Article  PubMed  CAS  Google Scholar 

  53. Elefanty AG, Hariharan IK, Cory S. ber-abl, the hallmark of chronic myeloid leukemia in man, induces multiple hemopoietic neoplasms in mice. EMBO J 1990; 9: 1069–1078.

    PubMed  CAS  Google Scholar 

  54. Voncken JW, Griffiths S, Greaves MF et al. Restricted oncogenicity of BCR-ABL p190 in transgenic mice. Cancer Res 1992; 52: 4534–39.

    PubMed  CAS  Google Scholar 

  55. Donehower LA, Harvey M, Slagle BL et al. Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours. 1992; 356: 215–21.

    CAS  Google Scholar 

  56. Dick J. Immune-deficient mice as models of normal and leukemic human hematopoiesis. Cancer Cells 1991; 3: 39–48.

    PubMed  CAS  Google Scholar 

  57. Kamel-Reid S, Letarte M, Sirard C et al. A model of human acute lymphoblastic leukemia in immune-deficient SCID mice. Science 1989; 246: 1597–1600.

    Article  PubMed  CAS  Google Scholar 

  58. Kamel-Reid S, Letarte M, Doedens M et al. Bone marrow from children in relapse with pre-B acute lymphoblastic leukemia proliferates and disseminates rapidly in scid mice. Blood 1991; 78: 2973–81.

    PubMed  CAS  Google Scholar 

  59. Cesano A, O’Connor R, Lange B et al. Homing and progression patterns of childhood acute lymphoblastic leukemias in severe combined immunodeficiency mice. Blood 1991; 77: 2463–74.

    PubMed  CAS  Google Scholar 

  60. Uckun FM, Downing JR, Gunther R et al. Human t(1;19)(q23;p13) pre-B acute lymphoblastic leukemia in mice with severe combined immunodeficiency. Blood 1993; 81: 3052–62.

    PubMed  CAS  Google Scholar 

  61. Sawyers C, Gishizky M, Quan S et al. Propagation of human blastic myeloid leukemias in the SCID mouse. Blood 1992; 79: 2089–98.

    PubMed  CAS  Google Scholar 

  62. Griffin DJ, Löwenberg B. Clonogenic cells in acute myeloblastic leukemia. Blood 1986; 68: 1185–95.

    PubMed  CAS  Google Scholar 

  63. Hu JP, Cesano A, Santoli D et al. Effects of interleukin-11 on the proliferation and cell cycle status of myeloid leukemic cells. Blood 1993; 81: 1586–92.

    PubMed  CAS  Google Scholar 

  64. Lapidot T, Sirard C, Vormoor J et al. A cell initiating human acute myeloid leukemia after transplantation into SCID mice. Nature 1994; 367: 645–48.

    Article  PubMed  CAS  Google Scholar 

  65. McCulloch E. Stem cells in normal and leukemic hemopoiesis (Henry Stratten Lecture). Blood 1983; 62: 1–13.

    PubMed  CAS  Google Scholar 

  66. McCulloch EA, Minden MD, Miyauchi J et al. Stem cell renewal and differentiation in acute myeloblastic leukemia. J Cell Sci Suppl 1988; 10: 267–81.

    Article  PubMed  CAS  Google Scholar 

  67. Porter EH, Berry RJ. The efficient design of transplantable tumor assays. Brit J Cancer 1964; 17: 583–95.

    Article  Google Scholar 

  68. Terstappen LWMM, Safford M, Unterhalt M et al. Flow cytometric characterization of acute myeloid leukemia: IV. Comparison to the differentiation pathway of normal hematopoietic progenitor cells. Leukemia 1992; 6: 993–1000.

    PubMed  CAS  Google Scholar 

  69. Terstappen LWWM, Huang S, Safford M et al. Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34’CD38– progenitor cells. Blood 1991; 77: 1218–27.

    PubMed  CAS  Google Scholar 

  70. Huang S, Terstappen LWMM. Lymphoid and myeloid differentiation of single human CD34’, HLA-DR’, CD38– hematopoietic stem cells. Blood 1994; 83: 1515–1526.

    PubMed  CAS  Google Scholar 

  71. Lapidot T, Cohen A, Grunberger T et al. Aberrant growth properties of juvenile chronic myelogenous leukemia (JCML) CD34’ cells in vitro and in vivo using SCID mouse assays. Blood 1993; 82 (Suppl 1): 197a.

    Google Scholar 

  72. Freedman MH, Estrov Z, Chan HSL. Juvenile chronic myelogenous leukemia. Am J Ped Hemat Oncol 1988; 10: 261–67.

    Article  CAS  Google Scholar 

  73. Freedman MH, Cohen A, Grunberger T et al. Central role of tumor necrosis factor, GM-CSF, and interleukin 1 in the pathogenesis of juvenile chronic myelogenous leukemia. Br J Haematol 1992; 80: 40–48.

    Article  PubMed  CAS  Google Scholar 

  74. Rowley JD. The Philadelphia chromosome translocation - A paradigm for understanding leukemia (Charles S. Mott Prize). Cancer 1990; 65: 2178–84.

    Article  PubMed  CAS  Google Scholar 

  75. Witte ON. Role of the ber-abl oncogene in human leukemia: fifteenth Richard and Hinda Rosenthal foundation award lecture. Cancer Res 1993; 53: 485–89.

    PubMed  CAS  Google Scholar 

  76. Jonas D, Lübbert M, Kawasaki ES et al. Clonal analysis of her-abl rearrangement in T lymphocytes from patients with chronic myelogenous leukemia. Blood 1992; 79: 1017–23.

    PubMed  CAS  Google Scholar 

  77. Eaves C, Eaves A. Cell culture studies in CML. Baillere’s Clin Haematol 1987; 1: 931–61.

    Article  CAS  Google Scholar 

  78. Dunbar CE, Stewart FM. Separating the wheat from the chaff: Selection of benign hematopoietic cells in chronic myeloid leukemia. Blood 1992; 79: 1107–10.

    PubMed  CAS  Google Scholar 

  79. Udomsakdi C, Eaves CJ, Swolin B et al. Rapid decline of chronic myeloid leukemic cells in long-term culture due to a defect at the leukemic stem cell level. Proc Natl Acad Sci USA 1992; 89: 6192–96.

    Article  PubMed  CAS  Google Scholar 

  80. Bock TA, Dunbar CE, Orlic D et al. Transgenic SCID mice containing the human IL-3, GM-CSF, and SCF genes as a model for human hematopoiesis. Blood 1993; 82 (Suppl. 1): 457a.

    Google Scholar 

Download references

Authors
  1. Josef Vormoor
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tsvee Lapidot
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. André Larochelle
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. John E. Dick
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Vormoor, J., Lapidot, T., Larochelle, A., Dick, J.E. (1995). Human Hematopoiesis in SCID Mice. In: Human Hematopoiesis in SCID Mice. Medical Intelligence Unit. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-22008-5_10

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-22008-5_10

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-22010-8

  • Online ISBN: 978-3-662-22008-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation