Abstract
Standardized sequence and structure analysis of antibody using IMGT®, the international ImMunoGeneTics information system® (http://www.imgt.org), allows to bridge, for the first time, the gap between antibody sequences and three-dimensional (3D) structures. This is achieved through the IMGT Scientific chart rules, based on IMGT-ONTOLOGY. IMGT® is the international reference for immunogenetics and immunoinformatics, and its standards (IMGT nomenclature and labels and IMGT unique numbering) are particularly useful for antibody humanization and evaluation of immunogenicity. In IMGT®, the antibody analysis from nucleotide sequence to 2D structure is supported by the widely used IMGT/V-QUEST system and the integrated IMGT/JunctionAnalysis software. The IMGT® approach from amino acid sequence to 3D structure is supported by the IMGT/DomainDisplay, IMGT/DomainGapAlign and IMGT/Collier-de-Perles tools, by some important features of IMGT/2Dstructure-DB, for antibodies for which 3D structures are not yet available (INN entries) and IMGT/3Dstructure-DB and associated tools, for crystallized antibodies (Contact analysis, IMGT/StructuralQuery, IMGT/DomainSuperimpose).
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Acknowledgements
We thank Chantal Ginestoux for the figures. We are very grateful to Gérard Lefranc for helpful discussions and to the IMGT® team for its expertise and constant motivation. IMGT® is a registered trademark of CNRS. IMGT® is member of the International Medical Informatics Association (IMIA). IMGT® was funded in part by the BIOMED1 (BIOCT930038), Biotechnology BIOTECH2 (BIO4CT960037) and 5th PCRDT Quality of Life and Management of Living Resources programmes (QLG2-2000-01287) programmes of the European Union (EU). IMGT® is currently supported by the Centre National de la Recherche Scientifique (CNRS), the Ministère de l'Enseignement Supérieur et de la Recherche (MESR) (Université Montpellier 2 Plan Pluri-Formation, Institut Universitaire de France), the GIS IBiSA, the Région Languedoc-Roussillon (Grand Plateau Technique pour la Recherche Région (GPTR) Languedoc-Roussillon), the Agence Nationale de la recherche ANR (BIOSYS06_135457, FLAVORES) and the EU 6th PCRDT ImmunoGrid (FP6 IST-028069).
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Ehrenmann, F., Duroux, P., Giudicelli, V., Lefranc, MP. (2010). Standardized Sequence and Structure Analysis of Antibody Using IMGT® . In: Kontermann, R., Dübel, S. (eds) Antibody Engineering. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-01147-4_2
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DOI: https://doi.org/10.1007/978-3-642-01147-4_2
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