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Extracellular RNAs: A New Awareness of Old Perspectives

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Extracellular RNA

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1740))

Abstract

Extracellular RNA (exRNA) has recently expanded as a highly important area of study in biomarker discovery and cancer therapeutics. exRNA consists of diverse RNA subpopulations that are normally protected from degradation by incorporation into membranous vesicles or by lipid/protein association. They are found circulating in biofluids, and have proven highly promising for minimally invasive diagnostic and prognostic purposes, particularly in oncology. Recent work has made progress in our understanding of exRNAs—from their biogenesis, compartmentalization, and vesicle packaging to their various applications as biomarkers and therapeutics, as well as the new challenges that arise in isolation and purification for accurate and reproducible analysis. Here we review the most recent advancements in exRNA research.

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Acknowledgements

B.G. is an Edward R. and Anne G. Lefler Center Postdoctoral Fellow. The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Department of Neurosurgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA

    Noah Sadik, Alessandra Gurtner & Leonora Balaj

  2. Department of Neurology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA

    Lilian Cruz, Sophie A. Dusoswa, Thomas Sebastiaan Van Solinge, Bence Gyorgy & Marike Broekman

  3. Department of Life Sciences, University of Trieste, Trieste, Italy

    Alessandra Gurtner

  4. Cardiovascular Division of the Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA

    Rodosthenis S. Rodosthenous, Olivia Ziegler & Ane Miren Salvador-Garicano

  5. Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands

    Sophie A. Dusoswa

  6. Amsterdam Infection & Immunity Institute, VU University Medical Center, Amsterdam, The Netherlands

    Sophie A. Dusoswa

  7. Cancer Center Amsterdam, VU University Medical Center, Amsterdam, The Netherlands

    Sophie A. Dusoswa

  8. NeuroDiscovery Center, Harvard Medical School, Boston, MA, USA

    Thomas Sebastiaan Van Solinge

  9. Department of Neurosurgery, VU University Medical Center, Amsterdam, The Netherlands

    Thomas Sebastiaan Van Solinge

  10. Department of Neurology, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, USA

    Zhiyun Wei

  11. Department of Neurosurgery, Brain Center Rudolf Magnus University Medical Center Utrecht, Utrecht, The Netherlands

    Marike Broekman

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  1. Noah Sadik
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Correspondence to Leonora Balaj .

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  1. Department of Transplantation, Mayo Clinic, Jacksonville, Florida, USA

    Tushar Patel

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Sadik, N. et al. (2018). Extracellular RNAs: A New Awareness of Old Perspectives. In: Patel, T. (eds) Extracellular RNA. Methods in Molecular Biology, vol 1740. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7652-2_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7652-2_1

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7651-5

  • Online ISBN: 978-1-4939-7652-2

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