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Molecular analysis and chromosomal mapping of amplified genes isolated from a transformed mouse 3T3 cell line

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Somatic Cell and Molecular Genetics

Abstract

We are exploring the origin and function of amplified DNA sequences associated with double minutes (DMs) in a spontaneously transformed derivative of mouse 3T3 cells. Toward that goal, we have constructed a cDNA library using RNA from these cells and have isolated cDNA clones representing sequences that are amplified and overexpressed in these 3T3-DM cells. From results of Northern- and Southern-blot analyses, we conclude that these cDNAs represent two distinct genes, which we have designated mdm-1and mdm-2.Using DNAs from a panel of Chinese hamster-mouse somatic cell hybrids together with in situ hybridization protocols for gene mapping studies, we have found that these DM-associated, amplified DNA sequences originate from mouse chromosome 10, region C1–C3. Sequences homologous to mdm-1and mdm-2are present in the genomes of several species examined, including that of man.

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Authors and Affiliations

  1. Department of Human Genetics, University of Pennsylvania, School of Medicine, 19104, Philadelphia, Pennsylvania

    Linda Cahilly-Snyder & Donna L. George

  2. Department of Human Genetics, Yale University, School of Medicine, 06510, New Haven, Connecticut

    Teresa Yang-Feng & Uta Francke

  3. Howard Hughes Medical Institute, University of Pennsylvania, School of Medicine, 19104, Philadelphia, Pennsylvania

    Donna L. George

Authors
  1. Linda Cahilly-Snyder
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  2. Teresa Yang-Feng
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  3. Uta Francke
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  4. Donna L. George
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Cahilly-Snyder, L., Yang-Feng, T., Francke, U. et al. Molecular analysis and chromosomal mapping of amplified genes isolated from a transformed mouse 3T3 cell line. Somat Cell Mol Genet 13, 235–244 (1987). https://doi.org/10.1007/BF01535205

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  • DOI: https://doi.org/10.1007/BF01535205

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