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opened on Mar 23, 2020
Hello,
First of all, thank you so much for collating this pipeline together. It has been a tremendous help in my research and in actually receiving interpretable results from alignment and QC of sequencing data.
ENCODE has specific guidelines for how QC and ATAC-seq data should be processed. One of the necessary metrics is PCR bottlenecking coefficient 1 and 2 (PBC1/PBC2). I've been looking through the output files from the nfcore-atacseq run pipeline in ataqv and multiQC, and can't seem to find anything on this. Is this something we'll have to calculate using the merged bam files? Thanks for the help!
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