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How should I choose all markers? #1
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hi Until then, please see below and feel free to post any further questions! the specScore matrix can be used to rank genes in each clusters, to estimate a cutoff to get all variable (differentially expressed genes, DEGs) I would go for the "adjpval" matrix from getPValues. if "sg" is the output of "sortGenes()", this will give you the top 10 genes in each cluster: to get a small set of marker genes: To get all variable genes: to get all marker genes for say cluster 1: |
Thanks for the prompt reply, actually, I have already done what you said. Best wishes, Zorro |
hi there is now a new function in the latest release called getTable to summarize variable genes and fold change values. I would recommend now to run something like this to get all variable genes:
to get a small list of marker genes: I'm closing the issue now, however feel free to reopen or open a new one if you have any further questions. best wishes |
Hi,
Thanks very much for such a wonderful tool to quickly find the DEGs.
However, if I want to extract all the DEGs of a cluster or all cluster, what cutoff of specScore should I use, or I have to use the adj_pvalue to measure? And if I use the pvalue, I have to extract the DEGs by myself, kind of not convenient, will you consider adding this function?
Looking forward to your reply!
Zorro
20190706
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