Update README.md

xie186 · Jul 22, 2022 · 94431cf · 94431cf
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@@ -11,7 +11,7 @@ http://htmlpreview.github.io/?https://raw.githubusercontent.com/xie186/MutFinder
 
 ![image](https://user-images.githubusercontent.com/20909751/129656288-3beeef69-f40c-4616-9621-74bc7e2019f3.png)
 
-WT plants were subjected to EMS mutagenesis. Individuals with desired phenotype in M2 plants were selected to cross WT plants. In the F2 generation, 120 plants with desired phenotype were collected and mixed together for genomic DNA isolation. 
+WT plants were subjected to EMS mutagenesis. Individuals with desired phenotype in M2 plants were selected to cross with WT plants. In the F2 generation, 120 plants with desired phenotype were collected and mixed together for genomic DNA isolation. 
 
 Re-sequencing reads from wild type and F2 generation were aligned to TAIR10 reference genome using bwa. SAM files generated were then converted to BAM files. PCR duplications were removed using MarkDuplicates.jar in Picard (http://broadinstitute.github.io/picard/). HaplotypeCaller in GATK was then used to identify the SNPs in both wild type and mutant samples. We chose the SNPs meet the following requirements: 1) showing homozygous reference allele in wild type; 2) showing C->T or G->A mutation between wild type and mutant samples; 3) showing ratios of the reads with alternative alleles/read depth > 0.3.