diff --git a/packages/vite/src/node/plugins/define.ts b/packages/vite/src/node/plugins/define.ts
index a076e0812a0bc1..11135dcb03b0d6 100644
--- a/packages/vite/src/node/plugins/define.ts
+++ b/packages/vite/src/node/plugins/define.ts
@@ -6,6 +6,7 @@ import { isCSSRequest } from './css'
 import { isHTMLRequest } from './html'
 
 const nonJsRe = /\.json(?:$|\?)/
+const metaEnvRe = /import\.meta\.env\.(.+)/
 const isNonJsRequest = (request: string): boolean => nonJsRe.test(request)
 
 export function definePlugin(config: ResolvedConfig): Plugin {
@@ -30,10 +31,17 @@ export function definePlugin(config: ResolvedConfig): Plugin {
     })
   }
 
+  const env = { ...config.env }
   const userDefine: Record<string, string> = {}
   for (const key in config.define) {
     const val = config.define[key]
     userDefine[key] = typeof val === 'string' ? val : JSON.stringify(val)
+
+    // make sure `import.meta.env` object has user define properties
+    const match = key.match(metaEnvRe)
+    if (match) {
+      env[match[1]] = val
+    }
   }
 
   // during dev, import.meta properties are handled by importAnalysis plugin.
@@ -41,10 +49,8 @@ export function definePlugin(config: ResolvedConfig): Plugin {
   const importMetaKeys: Record<string, string> = {}
   const importMetaFallbackKeys: Record<string, string> = {}
   if (isBuild) {
-    const env: Record<string, any> = {
-      ...config.env,
-      SSR: !!config.build.ssr,
-    }
+    env.SSR = !!config.build.ssr
+
     // set here to allow override with config.define
     importMetaKeys['import.meta.hot'] = `undefined`
     for (const key in env) {
@@ -52,7 +58,7 @@ export function definePlugin(config: ResolvedConfig): Plugin {
     }
     Object.assign(importMetaFallbackKeys, {
       'import.meta.env.': `({}).`,
-      'import.meta.env': JSON.stringify(config.env),
+      'import.meta.env': JSON.stringify(env),
     })
   }
 
diff --git a/playground/env/__tests__/env.spec.ts b/playground/env/__tests__/env.spec.ts
index 227f6b5c09ccfe..dc4a32653efdab 100644
--- a/playground/env/__tests__/env.spec.ts
+++ b/playground/env/__tests__/env.spec.ts
@@ -49,6 +49,10 @@ test('expand', async () => {
   expect(await page.textContent('.expand')).toBe('expand')
 })
 
+test('ssr', async () => {
+  expect(await page.textContent('.ssr')).toBe('false')
+})
+
 test('env object', async () => {
   const envText = await page.textContent('.env-object')
   expect(JSON.parse(envText)).toMatchObject({
@@ -56,6 +60,8 @@ test('env object', async () => {
     CUSTOM_PREFIX_ENV_VARIABLE: '1',
     VITE_CUSTOM_ENV_VARIABLE: '1',
     BASE_URL: '/env/',
+    VITE_BOOL: true,
+    SSR: false,
     MODE: mode,
     DEV: !isBuild,
     PROD: isBuild,
diff --git a/playground/env/index.html b/playground/env/index.html
index 6ccfd421945418..9b698ff237e426 100644
--- a/playground/env/index.html
+++ b/playground/env/index.html
@@ -15,6 +15,7 @@ <h1>Environment Variables</h1>
 <p>typeof import.meta.env.VITE_BOOL: <code class="bool"></code></p>
 <p>process.env.NODE_ENV: <code class="node-env"></code></p>
 <p>import.meta.env.VITE_EXPAND: <code class="expand"></code></p>
+<p>import.meta.env.SSR: <code class="ssr"></code></p>
 <p>import.meta.env: <span class="pre env-object"></span></p>
 <p>import.meta.url: <span class="pre url"></span></p>
 
@@ -28,6 +29,7 @@ <h1>Environment Variables</h1>
   text('.mode-file', import.meta.env.VITE_EFFECTIVE_MODE_FILE_NAME)
   text('.inline', import.meta.env.VITE_INLINE)
   text('.bool', typeof import.meta.env.VITE_BOOL)
+  text('.ssr', import.meta.env.SSR)
   text('.node-env', process.env.NODE_ENV)
   text('.env-object', JSON.stringify(import.meta.env, null, 2))
   text('.expand', import.meta.env.VITE_EXPAND)