Description
Hi, as the subject indicates, I was able to run the proc10X script just fine. However, when I move on to filtering, I get the following error:
My headers look like this after running proc10X:
FILTER NOTE Finished reading in barcode file
FILTER FILES mylib_process_10xReads_R1_001.fastq,mylib_process_10xReads_R2_001.fastq
FILTER ERROR:[TwoReadIlluminaRun] Error reading next read
Traceback (most recent call last):
File "/data/tigrr/home/cfeigin/src/proc10xG/filter_10xReads.py", line 490, in main
fragment = iterator.next_processed()
File "/data/tigrr/home/cfeigin/src/proc10xG/filter_10xReads.py", line 206, in next_processed
id1 = self.R1.next().strip()
AttributeError: '_io.TextIOWrapper' object has no attribute 'next'
FILTER ERROR An unknown fatal error was encountered.
@GCGCCAACACATCCAA:A00741:43:HC375DRXX:2:2101:1063:1016 1:N:0:0:MISMATCH1_GNGCCAACACATCCAA_F#FFFFFFFFFFFFFF_AAACAGC_FFFFFFF
I was a bit unclear about the delimiters on the main page. Its odd that one script could parse the headers and one couldn't though.
Activity