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Update README.md
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enovoa authored Aug 29, 2020
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Expand Up @@ -190,7 +190,8 @@ This is optional. Users who are interested in exploring the electric signals inc

Note 1: Please add the /path/to/nanopolish to environmental **$PATH** variable, otherwise the script will fail.
```
sh Epinano_Current.sh -h
$ sh Epinano_Current.sh -h
Epinano_Current.sh [-h] [-b bam -r reads -f genome/transriptome reference -d fast5dir -t threads -m bam_file_mapping_type]
it runs nanopolish eventalign; aggreagets current intensity values associated with single positions
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Note 1: different types of RNA base modification show distinct biases toward the spefic types of errors. Thus, offered *Epinano_sumErr.py* to combine mismatches, indels and even quality scores. Just like the independent types of errors, the combined error is internally performed when running *Epinano_ErrDiff.R*.
```
Rscript Epinano_DiffErr.R -h
$ Rscript Epinano_DiffErr.R -h
Usage:
DiffErr.R v0.1 compares a given feature between two samples. It predict potential modified sites mainly through two methods:
1. compute deviance of selected featuers between samples and then calculate z-scores. Outliers or potential modified sites will then
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EpiNano 1.2 includes pre-trained models (found in *$EPINANO_HOME/models/*), which have been trained using synthetic molecules (curlcakes) with and without introduced m6A modifications. However, the user can train their own models using **EpiNano_Predict**, employing the features generated with *EpiNano_Variants.py* and/or *EpiNano_Current.py* as shown in the previous steps. The relevant commands can be found in *test_data/train_models/*.

```
python Epinano_Predict.py -h
$ python Epinano_Predict.py -h
Command: Epinano_Predict.py -h
usage: Epinano_Predict.py [-h] [-k KERNEL] [-o OUT_PREFIX] [-a] [-M MODEL]
[-t TRAIN] [-mc MODIFICATION_STATUS_COLUMN] -p
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