Pfeature is a comprehensive software developed for computing wide range of protein/peptide features that have been discovered over the past decades. It has the following five major modules for computing protein features based on; i) Composition, ii) Binary profiles, iii) Evolutionary information iv) Structure and v) Pattern. The composition based module allows user to compute; i) Simple compositions like amino acid, dipeptide, tripeptide; ii) Physicochemical properties based compositions; iii) Repeats and distribution of amino acids; iv) Shannon entropy to measure the low complexity regions; iv) Miscellaneous compositions like pseudo amino acid, autocorrelation, conjoint triad, quasi-sequence order. Binary profile of amino acid sequence provides complete information including order of residues or type of residues, which is not possible with composition based features. Thus, binary profile can be used to annotate protein at residue level. It is well established in literature that sequence profile based on evolutionary information provides more information then sequence itself.

We have developed number of isoforms of Pfeature that include: i) A web server that uses Pfeature functions via web interface from https://webs.iiitd.edu.in/raghava/pfeature/ ; ii) Standalone version of Pfeature; iii) Library of python for Pfeature and iv) Python scripts for computing features.

One can read more about subroutines developed under Pfeature to compute wide range of proteins and peptide features from https://github.com/raghavagps/Pfeature/blob/master/Pfeature_Man.pdf . Further information is available from help page of web site https://webs.iiitd.edu.in/raghava/pfeature/help.php

A web server for computing wide range of protein and peptides features from their amino acid sequences. Following are main menus for computing features; i) Composition-based features, ii) Binary profile of sequences, iii) evolutionary information based features, iv) structural descriptors,and v) pattern based descriptors, for a group of protein/peptide sequences. Additionally, users will also be able to generate these features for sub-parts of protein/peptide sequences. Pfeature will be helpful to annotate structure, function and therapeutic properties of proteins/peptides.

Available from URL: https://webs.iiitd.edu.in/raghava/pfeature/

PIP version is also available for easy installation and usage of this tool. The following command is required to install the package

pip install pfeature

To know about the available option for the pip package, type the following command:

pfeature -h

The prerequisite to run the python library is pandas, numpy and python version above 3.6 pandas can be installed using following command: pip3 install pandas numpy can be installed using following command: pip3 install numpy

It has been tested on wide range of platforms that include Apple MAC, Windows and Linux (Ubuntu,Fedora). After installing pandas and numpy user can install using following commands

1. Download Pfeature from https://github.com/raghavagps/Pfeature/blob/master/PyLib/Pfeature.zip
   
2. Extract or uncompress Pfeature.zip
   
3. cd Pfeature
   
4. python setup.py install
   

In order to facilitate users, we created a single program of Pfeature which computes individual as well as, all possible descriptors for a protein/peptide sequence. It has been tested on wide range of platforms that include Apple MAC, Windows and Linux (Ubuntu,Fedora). After installing pandas and numpy user can install using following commands

1. Download Pfeature from https://github.com/raghavagps/Pfeature/blob/master/Standalone/pfeature_standalone.zip
   
2. Extract or uncompress pfeature_standalone.zip
   
3. cd pfeature_standalone
   

Pande et al (2022) Pfeature: A Tool for Computing Wide Range of Protein Features and Building Prediction Models. J Comput Biol. 2022 Oct 13. doi: 10.1089/cmb.2022.0241.