Tested minimap 2.22 & 2.24 with the options modified from -x splice:

-k15 -w5 --splice -g2k -G200k -A1 -B2 -O2,32 -E1,0 -b0 -C9 -z200 -ub --junc-bonus=9 --cap-sw-mem=0 --splice-flank=no

I got: [ERROR] unknown option in "-E1,0"

Please advise. Thanks!