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c-thoben authored May 6, 2023
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The bHLH_annotator allows the automatic identification and functional annotation of the bHLH transcription factor family in novel plant sequence data sets. Coding sequences or peptide sequences derived from a de novo genome and transcriptome assembly can be analyzed with this pipeline.

A phylogenetic approach is performed for the annotation of the candidates, based on a [bait collection](https://github.com/bpucker/bHLH_annotator/blob/main/supplements/) of bHLHs and outgroup sequences (non-bHLHs with a high sequence similarity to bHLHs):
A phylogenetic approach is performed for the annotation of the candidates, based on a [bait collection](https://github.com/bpucker/bHLH_annotator/blob/main/supplements/) of bHLHs and outgroup sequences (non-bHLHs with a high sequence similarity to bHLHs):

<img alt="bHLH_annotator steps" src="https://github.com/bpucker/bHLH_annotator/blob/main/bHLH_annotator.png?raw=true" width="50%" height="70%">

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