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bpucker authored Jul 10, 2022
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## Tools
### KIPEs
### Knowledge-based Identification of Pathway Enzymes (KIPEs)
Knowledge-based Identification of Pathway Enzymes (KIPEs) performs an automatic annotation of the flavonoid biosynthesis steps in a new transcriptome of genome sequence assembly. Scripts and datasets are availabe in the [KIPEs repository](https://github.com/bpucker/KIPEs). KIPEs is also available on [our web server](http://pbb.bot.nat.tu-bs.de/KIPEs/). This enables all scientists to use KIPEs in their research projects. Please find additional details in the corresponding publication ['Automatic Identification of Players in the Flavonoid Biosynthesis with Application on the Biomedicinal Plant <i>Croton tiglium</i>'](https://www.mdpi.com/2223-7747/9/9/1103/htm). Recently, we updated KIPEs with additional features and data sets as described in our preprint ['KIPEs3: Automatic annotation of biosynthesis pathways'](https://doi.org/10.1101/2022.06.30.498365).

<a href="https://github.com/bpucker/KIPEs">
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</a>


### MGSE
### Mapping-based Genome Size Estimation (MGSE)
Mapping-based Genome Size Estimation (MGSE) performs an estimation of a genome size based on a read mapping to an existing genome sequence assembly. Please find the script and detailed instructions in the [MGSE repository](https://github.com/bpucker/MGSE). Additional details are described in the corresponding publication ['Mapping-based genome size estimation'](https://doi.org/10.1101/607390).

<a href="https://github.com/bpucker/MGSE">
<img alt="MGSE workflow (Pucker, 2021; doi:10.1101/607390)" src="https://raw.githubusercontent.com/bpucker/MGSE/df459458173ec0bcffba7ce61484e5765118c817/MGSE_concept.png" width="50%" height="50%">
<img alt="MGSE concept (Pucker, 2021; doi:10.1101/607390)" src="https://raw.githubusercontent.com/bpucker/MGSE/df459458173ec0bcffba7ce61484e5765118c817/MGSE_concept.png" width="50%" height="50%">
</a>


### Neighborhood-Aware Variant Impact Predictor (NAVIP)
Prediction of the functional consequences of sequence variants listed in a VCF file.
https://github.com/bpucker/NAVIP
server (under construction)
publication
Prediction of the functional consequences of sequence variants listed in a VCF file. Please find scripts and detailed instructions in the [NAVIP repository](https://github.com/bpucker/NAVIP). We are currently working to make NAVIP available through our web server. Please find additional details in the corresponding publication ['Influence of neighboring small sequence variants on functional impact prediction'](https://github.com/bpucker/NAVIP).

<a href="https://github.com/bpucker/NAVIP">
<img alt="cInDels detected by NAVIP (Baasner <i>et al</i>., 2019; doi:10.1101/596718 )" src="https://www.biorxiv.org/content/biorxiv/early/2019/06/27/596718/F3.large.jpg?width=800&height=600&carousel=1" width="50%" height="50%">
</a>

## Teaching

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