Multidomain, Automated, Photopatterning of DNA-Functionalized Hydrogels (MAPDH) is a platform for the automated fabrication of DNA-functionalized hydrogels using digital, maskless photolithography.
- The preprint can be found at https://doi.org/10.26434/chemrxiv-2023-qmfjq
- The MAPDH protocol can be found at https://dx.doi.org/10.17504/protocols.io.j8nlkw2ywl5r/v1
To streamline MAPDH use, example scripts and functions are provided.
Key files:
- Patterning_functions
- This file contains all necessary functions, with documentation, to run all operations in MAPDH.
- Patterning_example_script
- This file is an example that uses built-in masks to pattern differently shaped hydrogels.
- MAPDH_example_script
- This file is an example for fully automated MADPH - incorporating the flow controller, patterning setup, bright-field and fluorescence imaging into one script for multi-domain patterning and simultaneous imaging.
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Install lastest version of Micromanager
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Install lastest version of Pycromanager
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Python and other required packages, with version numbers:
- Python: 3.8
- anaconda: 2020.07
- Scikit-image: 0.19.3
- Pandas: 1.4.4
- numpy: 1.18.5
- PIL: 7.2.0
- Pycromanager: 0.6.0
These are real-time videos for fabrication of hydrogel letters taken using bright-field microscopy, with a field of view of 1250 microns. Each letter is fabricated using the message_mask_generator package in Python. Each letter here is the same domain, i.e., each hydrogel has the same composition.