diff --git a/docs/debug_notebooks/Comparing methylprep, sesame, minfi v1.5.2.ipynb b/docs/debug_notebooks/Comparing methylprep, sesame, minfi v1.5.2.ipynb new file mode 100644 index 0000000..8aaafbd --- /dev/null +++ b/docs/debug_notebooks/Comparing methylprep, sesame, minfi v1.5.2.ipynb @@ -0,0 +1,832 @@ +{ + "cells": [ + { + "cell_type": "code", + "execution_count": 1, + "id": "d9783444", + "metadata": {}, + "outputs": [], + "source": [ + "from pathlib import Path\n", + "import pandas as pd\n", + "import matplotlib.pyplot as plt\n", + "import methylprep\n", + "import methylcheck\n", + "from pprint import pprint as pp\n", + "mpath = Path('/Volumes/LEGX/GEO/test_pipeline/GSE105018_test') # methylprep --all sesame v1.5.1\n", + "m_minfi_path = Path('/Volumes/LEGX/GEO/test_pipeline/GSE105018_param_minfi') # methylprep --minfi (but no --all)\n", + "m_v111 = Path('/Volumes/LEGX/GEO/test_pipeline/GSE105018_v111') # methylprep --betas v1.1.1 2019 version\n", + "m_v146 = Path('/Volumes/LEGX/GEO/test_pipeline/GSE105018_v146') # methylprep --betas v1.4.6 March-2021 version --minfi\n", + "minpath = Path('/Volumes/LEGX/GEO/test_pipeline/GSE105018_minfi')\n", + "sespath = Path('/Volumes/LEGX/GEO/test_pipeline/GSE105018_sesame')\n", + "t1_path = Path('/Volumes/LEGX/GEO/test_pipeline/GSE105018_test_steps') # df = m.make_pipeline('.', steps=['noob'], sesame=False, debug=True)" + ] + }, + { + "cell_type": "markdown", + "id": "f2c0c068", + "metadata": {}, + "source": [ + "# ref on how minfi noob values were calculated\n", + "```\n", + "library(minfi)\n", + "library(minfiData)\n", + "\n", + "process_minfi = function(folder = '/Volumes/LEGX/GEO/test_pipeline/GSE105018_minfi') {\n", + " baseDir = paste0(folder)\n", + " targets = read.metharray.sheet(baseDir, 'samplesheet.csv')\n", + " RGset <- read.metharray.exp(targets = targets)\n", + " pd = pData(RGset) # to dataframe\n", + " raw = preprocessRaw(RGset) # converts to matrix of meth | unmeth without normalization\n", + " noob = preprocessNoob(RGset) # converts to matrix of meth | unmeth NOOB + linear dye-bias correction\n", + " beta = getBeta(noob) # no SNPS included\n", + " noob_meth = getMeth(noob)\n", + " noob_unmeth = getUnmeth(noob)\n", + " write.csv(getMeth(raw), file.path(folder, \"minfi_meth.csv\"), row.names=TRUE)\n", + " write.csv(getUnmeth(raw), file.path(folder, \"minfi_unmeth.csv\"), row.names=TRUE)\n", + " write.csv(getBeta(raw), file.path(folder, \"minfi_raw_beta.csv\"), row.names=TRUE)\n", + " write.csv(beta, file.path(folder, \"minfi_noob_beta.csv\"), row.names=TRUE)\n", + " write.csv(noob_meth, file.path(folder, \"minfi_noob_meth.csv\"), row.names=TRUE)\n", + " write.csv(noob_unmeth, file.path(folder, \"minfi_noob_unmeth.csv\"), row.names=TRUE)\n", + "}\n", + "\n", + "process_minfi('/Volumes/LEGX/GEO/test_pipeline/GSE105018_minfi')\n", + "\n", + "```" + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "id": "70c9c5d4", + "metadata": {}, + "outputs": [], + "source": [ + "mmeth = pd.read_pickle(Path(mpath,'noob_meth_values.pkl')).sort_index() # methylprep_noob_meth --sesame\n", + "munmeth = pd.read_pickle(Path(mpath,'noob_unmeth_values.pkl')).sort_index() # methylprep_noob_unmeth --sesame\n", + "\n", + "# minfi R-outputs\n", + "_meth = pd.read_csv(Path(minpath,'minfi_noob_meth.csv')).set_index('Unnamed: 0').sort_index()\n", + "_meth.index.name='IlmnID'\n", + "_unmeth = pd.read_csv(Path(minpath,'minfi_noob_unmeth.csv')).set_index('Unnamed: 0').sort_index()\n", + "_unmeth.index.name='IlmnID'\n", + "\n", + "# CLI methylprep with --minfi params\n", + "mmeth_minfi = pd.read_pickle(Path(minpath,'noob_meth_values.pkl')).sort_index()\n", + "munmeth_minfi = pd.read_pickle(Path(minpath,'noob_unmeth_values.pkl')).sort_index()\n", + "\n", + "# CLI methylprep with --all --minfi params ???\n", + "m_minfi_meth = pd.read_pickle(Path(m_minfi_path,'noob_meth_values.pkl')).sort_index() # methylprep_noob_meth --minfi\n", + "m_minfi_unmeth = pd.read_pickle(Path(m_minfi_path,'noob_unmeth_values.pkl')).sort_index() # methylprep_noob_unmeth --minfi\n", + "\n", + "m_v111_samples = {}\n", + "for _csv in Path(m_v111, '3999356047').rglob('*'):\n", + " sample_name = '_'.join(Path(_csv).stem.split('_')[:2])\n", + " sample = pd.read_csv(_csv).set_index('IlmnID')\n", + " m_v111_samples[sample_name] = sample\n", + "\n", + "m_v146_samples = {}\n", + "for _csv in Path(m_v146, '3999356047').rglob('*'):\n", + " sample_name = '_'.join(Path(_csv).stem.split('_')[:2])\n", + " sample = pd.read_csv(_csv).set_index('IlmnID')\n", + " m_v146_samples[sample_name] = sample\n", + "\n", + "t1_meth = pd.read_pickle(Path(t1_path,'noob_meth_values.pkl')).sort_index()\n", + "t1_unmeth = pd.read_pickle(Path(t1_path,'noob_unmeth_values.pkl')).sort_index()\n" + ] + }, + { + "cell_type": "markdown", + "id": "c231e530", + "metadata": {}, + "source": [ + "# methylprep v1.1.1 vs minfi (noob meth, noob unmeth)" + ] + }, + { + "cell_type": "code", + "execution_count": 3, + "id": "4ae32331", + "metadata": { + "scrolled": false + }, + "outputs": [ + { + "data": { + "image/png": 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VwLyyzkD7kCRJkjrS1OEsVM4y3wkcTnW2+QfAk5nZVxZZD0wv89OBRwEysy8itgEHlfja2mbr6zzaEj++rDPQPlrzWwwsBujq6qKnp2c4b+tF27dvH/E6TTOHzshhyVF9HZGHOZiDJGniGlaBnZkvAEdHxAHAV4A3jGZSI5WZy4BlALNnz87u7u4Rrd/T08NI12maOXRGDmcvvQGAq+buO+mPhTl0Vg6SpIljRKOIZOaTwC3ALwEHRER/gT4D2FDmNwCHApT2/YHN9XjLOgPFNw+yD0mSJKkjDWcUkdeUM9dExD7AO4AHqArt08tiC4Hry/yq8prS/vXMzBKfX0YZOQyYBdwG3A7MKiOG7El1I+Sqss5A+5AkSZI60nAuETkEWFGuw34FcG1m/lNE3A+sjIhPAt8FrizLXwl8LiJ6gS1UBTOZuS4irgXuB/qAc8ulJ0TEecBNwBRgeWauK9v6yAD7kCRJkjrSkAV2Zt4DvLlN/GGqEUBa4z8GzhhgWxcBF7WJrwZWD3cfkiRJUqfySY6SJElSgyywJUmSpAZZYEuSJEkNssCWJEmSGmSBLUmSJDXIAluSJElqkAW2JEmS1CALbEmSJKlBFtiSJElSgyywJUmSpAZZYEuSiIhDI+KWiLg/ItZFxAdL/MCIWBMRD5Wf00o8IuKyiOiNiHsi4pjathaW5R+KiIW1+LERcW9Z57KIiMH2IUkTlQW2JAmgD1iSmUcAc4BzI+IIYClwc2bOAm4urwFOAWaVaTFwBVTFMnA+cDxwHHB+rWC+Anhfbb25JT7QPiRpQrLAliSRmRsz864y/zTwADAdmAesKIutAE4r8/OAq7OyFjggIg4BTgbWZOaWzNwKrAHmlrb9MnNtZiZwdcu22u1DkiakqeOdgCSps0TETODNwK1AV2ZuLE2PAV1lfjrwaG219SU2WHx9mziD7KOe02KqM+V0dXXR09Mz4ve1ffv2nVqvSebQGTksOaqPrn2Y9MfBHEYvBwtsSdKLIuJVwJeAD2XmU+UyaQAyMyMiR3P/A+0jM5cBywBmz56d3d3dI952T08PO7Nek8yhM3I4e+kNLDmqjzMn+XEwh9HLwUtEJEkARMQeVMX1FzLzyyX8eLm8g/JzU4lvAA6trT6jxAaLz2gTH2wfkjQhWWBLkigjelwJPJCZn641rQL6RwJZCFxfiy8oo4nMAbaVyzxuAk6KiGnl5saTgJtK21MRMafsa0HLttrtQ5ImpCEL7EGGbrogIjZExN1lOrW2zkfLMEwPRsTJtfjcEuuNiKW1+GERcWuJXxMRe5b4XuV1b2mf2ei7lyT1eyvwLuCEln79YuAdEfEQ8PbyGmA18DDQC3wWOAcgM7cAFwK3l+kTJUZZ5h/KOj8AbizxgfYhSRPScK7B7h+66a6IeDVwZ0SsKW2XZuZf1RcuwzrNB44EXg98LSJ+rjRfDryD6uaW2yNiVWbeD1xStrUyIv4eWEQ1nNMiYGtmHh4R88tyv7Urb1iS9HKZ+W0gBmg+sc3yCZw7wLaWA8vbxO8A3tgmvrndPiRpohryDPYgQzcNZB6wMjOfy8wfUp2pOK5MvZn5cGY+D6wE5pWvCk8Arivrtw4D1T9003XAif0PJpAkSZI60YiuwW4ZugngvPIEr+W1BwmMdOimg4AnM7OvJb7Dtkr7trK8JEmS1JGGPUxfm6GbrqC6zi7Lz08B7xmVLIfObZfGR90dx180h52z5Ki+jsjDHMxBkjRxDavAbjd0U2Y+Xmv/LPBP5eVAQzQxQHwz1RPAppaz1PXl+7e1PiKmAvuX5Xewq+Oj7o7jL5rDzjl76Q0AXDV330l/LMyhs3KQJE0cwxlFpO3QTf1jlhbvBO4r86uA+WUEkMOAWcBtVHeTzyojhuxJdSPkqnKjzC3A6WX91mGg+oduOh34ellekiRJ6kjDOYPdP3TTvRFxd4l9DDgrIo6mukTkEeD9AJm5LiKuBe6nGoHk3Mx8ASAizqMaI3UKsDwz15XtfQRYGRGfBL5LVdBTfn4uInqBLVRFuSRJktSxhiywBxm6afUg61wEXNQmvrrdepn5MNUoI63xHwNnDJWjJEmS1Cl8kqMkSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSZIkqUFDFtgRcWhE3BIR90fEuoj4YIkfGBFrIuKh8nNaiUdEXBYRvRFxT0QcU9vWwrL8QxGxsBY/NiLuLetcFhEx2D4kSZKkTjWcM9h9wJLMPAKYA5wbEUcAS4GbM3MWcHN5DXAKMKtMi4EroCqWgfOB44HjgPNrBfMVwPtq680t8YH2IUlqUEQsj4hNEXFfLXZBRGyIiLvLdGqt7aPlpMiDEXFyLT63xHojYmktflhE3Fri10TEniW+V3ndW9pnjtFblqRRM2SBnZkbM/OuMv808AAwHZgHrCiLrQBOK/PzgKuzshY4ICIOAU4G1mTmlszcCqwB5pa2/TJzbWYmcHXLttrtQ5LUrKt46eRG3aWZeXSZVgOUkyzzgSPLOp+JiCkRMQW4nOpEyxHAWWVZgEvKtg4HtgKLSnwRsLXELy3LSdKENnUkC5czC28GbgW6MnNjaXoM6Crz04FHa6utL7HB4uvbxBlkH615LaY6W05XVxc9PT0jeVts3759xOs0zRw6I4clR/V1RB7mYA5jLTO/OYKzx/OAlZn5HPDDiOil+mYSoDczHwaIiJXAvIh4ADgB+H/KMiuAC6i+vZxX5gGuA/4uIqKccJGkCWnYBXZEvAr4EvChzHyqXCYNQGZmRIxqZzjYPjJzGbAMYPbs2dnd3T2ibff09DDSdZpmDp2Rw9lLbwDgqrn7TvpjYQ6dlcM4Oi8iFgB3UF0uuJXqJMja2jL1EyOtJ1KOBw4CnszMvjbLv3jyJTP7ImJbWf6JehK7eiIFOuM/SubQGTksOaqPrn2Y9MfBHEYvh2EV2BGxB1Vx/YXM/HIJPx4Rh2TmxnKZx6YS3wAcWlt9RoltALpb4j0lPqPN8oPtQ5I0+q4ALgSy/PwU8J7xSGRXT6RAZ/xHyRw6I4ezl97AkqP6OHOSHwdzGL0chjOKSABXAg9k5qdrTauA/pFAFgLX1+ILymgic4Bt5TKPm4CTImJaubnxJOCm0vZURMwp+1rQsq12+5AkjbLMfDwzX8jMnwCf5aXLQAY7kdIuvpnqfpypLfEdtlXa9y/LS9KENZxRRN4KvAs4oeVO8ouBd0TEQ8Dby2uA1cDDQC9Vh3wOQGZuoToDcnuZPlFilGX+oazzA+DGEh9oH5KkUVa+Oez3TqB/hJFVwPwyAshhVKM/3UbVt88qI4bsSXUj5KpyPfUtwOll/daTMv0nUk4Hvu7115ImuiEvEcnMbwMxQPOJbZZP4NwBtrUcWN4mfgfwxjbxze32IUlqVkR8keoyvoMjYj3VsKrdEXE01SUijwDvB8jMdRFxLXA/1VCu52bmC2U751F9YzkFWJ6Z68ouPgKsjIhPAt+l+maU8vNz5UbJLVRFuSRNaCMaRUSStHvKzLPahK9sE+tf/iLgojbx1VTfZLbGH+alS0zq8R8DZ4woWUnqcD4qXZIkSWqQBbYkSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJbauHfDtvFOQZIkTVAW2JIkSVKDLLAlSZKkBg1ZYEfE8ojYFBH31WIXRMSGiLi7TKfW2j4aEb0R8WBEnFyLzy2x3ohYWosfFhG3lvg1EbFnie9VXveW9pmNvWtJkiRplAznDPZVwNw28Usz8+gyrQaIiCOA+cCRZZ3PRMSUiJgCXA6cAhwBnFWWBbikbOtwYCuwqMQXAVtL/NKynCRJktTRhiywM/ObwJZhbm8esDIzn8vMHwK9wHFl6s3MhzPzeWAlMC8iAjgBuK6svwI4rbatFWX+OuDEsrwkSZLUsabuwrrnRcQC4A5gSWZuBaYDa2vLrC8xgEdb4scDBwFPZmZfm+Wn96+TmX0Rsa0s/0RrIhGxGFgM0NXVRU9Pz4jeyPbt20e8TtPMoTNyWHJU9avYtQ+T/liYQ2flIEmaOHa2wL4CuBDI8vNTwHuaSmqkMnMZsAxg9uzZ2d3dPaL1e3p6GOk6TTOHzsjh7KU3AFWhfeYkPxbm0Fk5SJImjp0aRSQzH8/MFzLzJ8BnqS4BAdgAHFpbdEaJDRTfDBwQEVNb4jtsq7TvX5aXJEmSOtZOFdgRcUjt5TuB/hFGVgHzywgghwGzgNuA24FZZcSQPaluhFyVmQncApxe1l8IXF/b1sIyfzrw9bK8JEmS1LGGvEQkIr4IdAMHR8R64HygOyKOprpE5BHg/QCZuS4irgXuB/qAczPzhbKd84CbgCnA8sxcV3bxEWBlRHwS+C5wZYlfCXwuInqpbrKcv6tvVpIkSRptQxbYmXlWm/CVbWL9y18EXNQmvhpY3Sb+MC9dYlKP/xg4Y6j8JEmSpE7ikxwlSQM9VOzAiFgTEQ+Vn9NKPCLisvIgsHsi4pjaOgvL8g9FxMJa/NiIuLesc1n/sKsD7UOSJjILbEkStH+o2FLg5sycBdxcXkP10LBZZVpMNbIUEXEg1WWEx1N9M3l+rWC+Anhfbb25Q+xDkiYsC2xJ0kAPFas/8Kv1QWBXZ2Ut1WhQhwAnA2syc0t5NsIaYG5p2y8z15ab1a+m/UPF6vuQpAlrVx40I0navXVl5sYy/xjQVeZffBBY0f+QsMHi69vEB9vHDnb1gWLQGQ8MMofOyGHJUX0+UMwcRjUHC2xJ0pAyMyNiVIdKHWwfu/pAMeiMBwaZQ2fkcPbSG3ygmDmMag5eIiJJGsjj/c89KD83lfhIHyq2ocy3xgfbhyRNWBbYkqSB1B/41fogsAVlNJE5wLZymcdNwEkRMa3c3HgScFNpeyoi5pTRQxbQ/qFi9X1I0oTlJSKSpIEeKnYxcG1ELAJ+BJxZFl8NnAr0As8C7wbIzC0RcSHV03sBPpGZ/TdOnkM1Usk+wI1lYpB9SNKEZYEtSRrooWIAJ7ZZNoFzB9jOcmB5m/gdwBvbxDe324ckTWReIiJJkiQ1yAJbkiRJapAFtiRJktQgC2xJkiSpQRbYkiRJUoMssCVJkqQGWWBLkiRJDbLAliRJkho0ZIEdEcsjYlNE3FeLHRgRayLiofJzWolHRFwWEb0RcU9EHFNbZ2FZ/qGIWFiLHxsR95Z1LiuP0R1wH5IkSVInG84Z7KuAuS2xpcDNmTkLuLm8BjgFmFWmxcAVUBXLVI/dPR44Dji/VjBfAbyvtt7cIfYhSZIkdawhC+zM/CawpSU8D1hR5lcAp9XiV2dlLXBARBwCnAysycwtmbkVWAPMLW37Zeba8ujdq1u21W4fkiRJUsfa2WuwuzJzY5l/DOgq89OBR2vLrS+xweLr28QH24ckSZLUsabu6gYyMyMim0hmZ/cREYupLkmhq6uLnp6eEW1/+/btI16naebQGTksOaoPgK59mPTHwhw6KwdJ0sSxswX24xFxSGZuLJd5bCrxDcChteVmlNgGoLsl3lPiM9osP9g+XiYzlwHLAGbPnp3d3d0DLdpWT08PI12naebQGTmcvfQGoCq0z5zkx8IcOisHSdLEsbOXiKwC+kcCWQhcX4svKKOJzAG2lcs8bgJOiohp5ebGk4CbSttTETGnjB6yoGVb7fYhSZIkdawhz2BHxBepzj4fHBHrqUYDuRi4NiIWAT8CziyLrwZOBXqBZ4F3A2Tmloi4ELi9LPeJzOy/cfIcqpFK9gFuLBOD7EOSJEnqWEMW2Jl51gBNJ7ZZNoFzB9jOcmB5m/gdwBvbxDe324ckSZLUyXySoyRJktQgC2xJkiSpQRbYkiRJUoMssCVJkqQGWWBLkiRJDbLAliRJkhpkgS1JkiQ1yAJbkiRJapAFtiRJktQgC2xJ0qAi4pGIuDci7o6IO0rswIhYExEPlZ/TSjwi4rKI6I2IeyLimNp2FpblH4qIhbX4sWX7vWXdGPt3KUnNscCWJA3Hr2bm0Zk5u7xeCtycmbOAm8trgFOAWWVaDFwBVUEOnA8cDxwHnN9flJdl3ldbb+7ovx1JGj0W2JKknTEPWFHmVwCn1eJXZ2UtcEBEHAKcDKzJzC2ZuRVYA8wtbftl5trMTODq2rYkaUKaOt4JSJI6XgL/HBEJ/M/MXAZ0ZebG0v4Y0FXmpwOP1tZdX2KDxde3ie8gIhZTnRGnq6uLnp6eEb+J7du379R6TTKHzshhyVF9dO3DpD8O5jB6OVhgS5KG8rbM3BARrwXWRMT3642ZmaX4HjWlqF8GMHv27Ozu7h7xNnp6etiZ9ZpkDp2Rw9lLb2DJUX2cOcmPgzmMXg5eIiJJGlRmbig/NwFfobqG+vFyeQfl56ay+Abg0NrqM0pssPiMNnFJmrAssCVJA4qIfSPi1f3zwEnAfcAqoH8kkIXA9WV+FbCgjCYyB9hWLiW5CTgpIqaVmxtPAm4qbU9FxJwyesiC2rYkaULyEhFJ0mC6gK+UkfOmAv87M78aEbcD10bEIuBHwJll+dXAqUAv8CzwboDM3BIRFwK3l+U+kZlbyvw5wFXAPsCNZZKkCcsCW5I0oMx8GHhTm/hm4MQ28QTOHWBby4HlbeJ3AG/c5WQlqUPs0iUiPnxAkiRJ2lET12D78AFJkiSpGI2bHH34gCRJkiatXb0Ge9wfPgC7/gCC3XGAc3PYOUuO6gPwAQTm0HE5SJImjl0tsMf94QNlP7v0AILdcYBzc9g5Zy+9AcAHEJhDx+UgSZo4dukSER8+IEmSJO1opwtsHz4gSZIkvdyuXCLiwwckSZKkFjtdYPvwAUmSJOnlRmOYPkmSJGnSssCWJEmSGmSBLUmSJDXIAluSJElqkAW2JEmS1CALbEmSJKlBFtiSJElSgyywJUmSpAZZYEuSJEkNssCWJEmSGmSBLUmSJDXIAluSJElqkAW2JEmS1CALbEmSJKlBFtiSJElSgyywJUmSpAZZYEuSJEkN6vgCOyLmRsSDEdEbEUvHOx9JUvPs6yXtTjq6wI6IKcDlwCnAEcBZEXHE+GYlSWqSfb2k3U1HF9jAcUBvZj6cmc8DK4F545yTJKlZ9vWSdiudXmBPBx6tvV5fYpKk3Yd9vaTdytTxTqAJEbEYWFxebo+IB0e4iYOBJ5rNasTMoXNy4Pfh4N//nXHPoxOOhTnsfA4/PRqJTFYN9PMwcX+XzGEU2M+bQ0M5tO3rO73A3gAcWns9o8R2kJnLgGU7u5OIuCMzZ+/s+k0wh87JoVPyMAdzmESG7Ot3tZ+Hzvh3NAdzMIfJkUOnXyJyOzArIg6LiD2B+cCqcc5JktQs+3pJu5WOPoOdmX0RcR5wEzAFWJ6Z68Y5LUlSg+zrJe1uOrrABsjM1cDqUd7NLn3t2BBzqHRCDtAZeZhDxRwmAfv6MWUOFXOomEOl0RwiM5vcniRJkjSpdfo12JIkSdKEMqkL7Ig4OiLWRsTdEXFHRBxX4hERl5VH9t4TEceMch6/FxHfj4h1EfEXtfhHSw4PRsTJo5lD2d+SiMiIOLi8HrPjEBF/WY7BPRHxlYg4oNY2ZsdhPB7XHBGHRsQtEXF/+R34YIkfGBFrIuKh8nPaGOQyJSK+GxH/VF4fFhG3luNxTbkBbTT3f0BEXFd+Fx6IiF8a6+MQEf+j/DvcFxFfjIi9x/o4qDn28y/LY9z6+bK/ce/rx6OfL/vtiL5+vPv5ss/dv6/PzEk7Af8MnFLmTwV6avM3AgHMAW4dxRx+FfgasFd5/dry8wjge8BewGHAD4Apo5jHoVQ3GP0IOHgcjsNJwNQyfwlwyVgfB6qbq34A/AywZ9nvEWPwe3gIcEyZfzXwL+V9/wWwtMSX9h+TUc7lw8D/Bv6pvL4WmF/m/x743VHe/wrgvWV+T+CAsTwOVA83+SGwT+39nz3Wx8Gp0X9T+/mX8hjXfr7sb1z7+vHq58u+O6KvH+9+vuxnt+/rJ/UZbCCB/cr8/sC/lfl5wNVZWQscEBGHjFIOvwtcnJnPAWTmploOKzPzucz8IdBL9Tjh0XIp8P9SHZN+Y3YcMvOfM7OvvFxLNQ5ufw5jdRzG5XHNmbkxM+8q808DD1D98c+j6oQoP08bzTwiYgbw34B/KK8DOAG4bixyiIj9gf8CXAmQmc9n5pOM8XGguvl7n4iYCrwS2MgYHgc1zn7+JePaz0NH9PXj0s9DZ/T1493Pl31Oir5+shfYHwL+MiIeBf4K+GiJj+Vje38O+JXylcQ3IuItY51DRMwDNmTm91qaxuvxxe+hOqMy1jmM++OaI2Im8GbgVqArMzeWpseArlHe/V9Tffj+pLw+CHiy9mE42sfjMODfgf9Vvr78h4jYlzE8Dpm5gaov+FeqznYbcCdjexzUrA9hP9+J/TyMT18/7v08jGtf/9eMbz8Pk6Sv7/hh+nZVRHwNeF2bpo8DJwL/IzO/FBFnUv1v6u1jnMNU4ECqr+beAlwbET8zxjl8jOpru1E1WA6ZeX1Z5uNAH/CF0c6n00TEq4AvAR/KzKeqEwuVzMyIGLUhfyLi14BNmXlnRHSP1n6GMBU4Bvi9zLw1Iv6G6mvCF43BcZhGdRblMOBJ4B+BuaO1PzXDfn5YOYxJPz9UHvb149fXd0g/D5Okr9/tC+zMHLAjjYirgQ+Wl/9I+cqEYT6ivaEcfhf4clYX/NwWET8BDh6rHCLiKKpfsO+VP/IZwF1R3Qg0Zseh5HI28GvAieV40HQOQxjLfe0gIvag6nC/kJlfLuHHI+KQzNxYvrLdNPAWdtlbgV+PiFOBvam+Uv8bqq+Lp5b/0Y/28VgPrM/MW8vr66g63bE8Dm8HfpiZ/w4QEV+mOjZjeRw0Qvbzg+cwlv38YHnU8jmb8evrx62fh3Hv6zuhn4dJ0tdP9ktE/g34r2X+BOChMr8KWBCVOcC22tcWTfs/VDfAEBE/R3Wx/xMlh/kRsVdEHAbMAm5reueZeW9mvjYzZ2bmTKpf/GMy8zHG8DhExFyqr61+PTOfrTWNyXEoxuVxzeUauCuBBzLz07WmVcDCMr8QuH60csjMj2bmjPI7MB/4emb+NnALcPoY5fAY8GhE/HwJnQjczxgeB6qvC+dExCvLv0t/DmN2HNQ4+/kO6eehI/r6cennYfz7+k7o50sek6OvzzG4c7ZTJ+BtVNfcfI/qOqhjSzyAy6nuNL4XmD2KOewJfB64D7gLOKHW9vGSw4OUu+DH4Jg8wkt3l4/lceilui7u7jL9/XgcB6o76v+l7O/jY/h7mMA9tfd/KtW1cTdTFQRfAw4co3y6eenu8p+h+pDrpTr7t9co7/to4I5yLP4PMG2sjwPwp8D3y9/k56hGNRjT4+DU6L+n/fzL8xmXfr7sb9z7+vHo52u/ix3R149nP1/2udv39T7JUZIkSWrQZL9ERJIkSWqUBbYkSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW9IuiYjuiFg/3nlIksZPRGREHD7eeXQKC+zdTER8PiI2RsRTEfEvEfHeWtt7I6I3IrZHxFcj4vW1tgMiYkVEbCrTBS3b/eWIuC0ino6IeyLibbW2X42IeyPiyYjYHBFfiYjptfarIuL5st/+aUqt/ZUR8ZmIeCIitkXEN2ttERGXlO1uLvPR5n0vKH/c9fd7Y8s+n4+Ie4dxDDMininrbIiIT7fku1dELC/H+LGI+HCtbU5ErImILRHx7xHxjxFxSMv2j4uI1eV4bSnH9d219hMj4vsR8WxE3BIRP11rOzMivlPaeoZ6L6PBTlQaffbl9uWdLCJ66v9GaiMznXajCTgS2KvMvwF4DDgW6AY2lfY9gSuAb9TW+1/APwKvBGYCPwDeXdoOBDYDZwBTgN8BtgLTSnsX8PoyvxfwF8Cq2ravAj45SM6fB1YCrynbP7bW9n7gQWAGMB24H/hAy/rTgO8D9wHvHWQ/PcCfDOMYJnB4mT8c2AC8r9b+58C3yn5/oRzjuaXtlHKc9ivHcjnw1dq6vwRsBz4CHAxE+fe5trQfDGwr29gb+EtgbW39twNnAn8C9IzT79iLx6e87gbWj/fvvpPT7jTZl9uXd/JU/g3e2xLb4bNhsk/jnoDTKP7jws8DG8sf8V8Bl9faXl/+GH62vH4CeEut/WPAt8r8rwHrWrb9L8CiNvvcq3Ra99diA3bKVB8cTwH7DdD+HWBx7fWieidVYn8PnNPuD762zEzgBWDmMI5bawF5bcux+zfgpNrrC4GVA2zrGODp2utv17fVZvnFwHdqr/cF/gN4Q8ty7x1up1zeewLvBh6l+kD9APAW4B7gSeDvWtZ5D/BAWfYm4KdL/JtlW8+UD5ffohTYwBKqD/6NlA90JyenXZ/sy3dYZjL35d20nMwAHgHeXuYvKO/xauBpYB0wu2XZPyz9/jPAlVT/qbqxLP81yn+2yvJzyr/bk8D3gO4Sv6j8G/y4fA78Xe14fwB4qKxzORDj/fczXpOXiOyGyld0z1KdCdgIrO5vqi9Wfr6xTax/fqC2l7VHxE9FxJNUHcgfUJ35qDunfIV2Z0T8Zi1+HPAj4E/L14r3trQfSfWH3e97Jda/3+OA2VQd82AWUH3IPDLEcjuIiDcAvwL0ltfTgEMGy6nFf6Hq5IiIV1Kd9bhukF3u8H4z8xmqM1ADbX8kjgdmURXFfw18nOosypHAmRHxX0ue86g+lH+D6kzUt4Avlnz+S9nWmzLzVZl5TXn9OmB/qjNTi4DLy7GStJPsy9uyLx/cr1N9i3AAsAr4u5b23wTeAfwc8N+piuuPUfX1rwB+H6BcGnQD8Emqbz7+APhSRLwmMz9O9blwXvkcOK+2/V+jOnnzi1T/ITy5+bc4MVhg74Yy8xzg1VSdyZeB54CvUhVRvxgR+1B9LZVUX31R2pdGxKujur72PbW2/w94fUScFRF7RMRC4Gdr7WTmv2bmAVRfi/0R1QdCv8uoCrvXAn8MXBURby1tM6g6921UZ2LOA1ZExC+U9leVtn7bgFeV6/mmAJ+h+iP/yRCHZQHV2ZfhuisinqE6i9tT9tOfT38e9Zxe3bqBiPhFquP8hyU0jepvbuMg+219vwNufydcmJk/zsx/pjp78cXM3JSZG6g6yzeX5T4A/HlmPpCZfcCfAUfXrx9s4z+BT2Tmf2bmaqqzGj/fQM7SpGVf3pZ9+eC+nZmrM/MF4HPAm1ra/zYzH6/1+7dm5ncz88fAV3jpc+B3gNVlWz/JzDXAHcCpQ+z/4sx8MjP/FbgFOLqh9zXhWGDvpjLzhcz8NlWn97uZ+TXgfOBLVF8TPUL1lVD/6A+/T3XG4iHgeqozluvLtjYD84APA48Dc6m+SnrZyBGZuQVYAVwfEVNL7K7M3JyZfaX4+gLV2VHKPv+T6mvH5zPzG1R/lCeV9u1U18D12w/YntX3UecA92Tm2sGORVQ38byOwc82tDqGqoP8Laozv/vW8unPo57T0y37PJzqzMAHM/NbJbwV+AnVWZOBtL7fttvfSY/X5v+jzev+D5yfBv6m3LjzJLCF6izXdAa2uRTj/Z6tbU/STrIvf4l9+bA8Vpt/Fti7/9+vGMnnwBn9nwPls+BtDP6e2+1/0n4OWGDv/qZSnaEgMy/PzFmZ2UXVOU+lupmEzNySmb+dma/LzCOpfjdu699IZn4jM9+SmQcC76K63u422ptKdYajtXN5cXO89DXlPQO091vHjv8Df1OJAZwIvLPc/f0Y8MvApyKi9SuxhcCXM3M7I5CVa6nO+vxJiW2lOmsxUE6UM71fozpj/Lna9p4t26p/bdpqh/cbEftS/futG3CN5j0KvD8zD6hN+2Tmd8YwB0k7si+3L3+G2rcN5cz/a3Zhe4N5FPhcy+fAvpl5cWnPwVYW3uS4O01UHeF8qv8xTqG69ukZqmuy9qb6+i6An6L6quzPauv+LHBQWe8Uqhtljqy1vxnYg6qj/Wvg/9bafoPqcoBXUP2xXwvcVWs/veT0CqqzGU/z0s0Se1BdE/fHVJ35W0v7G0r7B6i+2ptO9bXjOsqd51TXmL2uNn2H6szM/rV970P1tdwJIziOrTfGHFWO4+vK64uBb1B9TfgGqk66/87z6VTX2f3BANv+ZaozG38IHFRib6LcWFOO3zaqjntv4BJ2vPN8Sol/gOqGw72BPYZ4PzPLe5pai63v/zcorz8P/FGZfyfVh/WR5fX+wBm1ZR9jxxuDuhnkxhsnJ6eRTdiX25e33+f+VGeF/1s53ucDfex4k+Pna8vPpNb309IvU/X7F9Revxf4Wpk/lKqvP7mWazcwo7SvpPZ7N8DxvopBRp3Z3adxT8CpwX/M6g/6G1R37z4F3EsZkqh0YP13Dj9GdXf4lNq6Z1LdUf0scDdwcsu2v1g6i23ANcBra22/B/ywtu2VlFEnSvu3ynpPUd30Mb9l20dSnQ14hmropnfW2oLqJpstZfoLBrgrmfbDBp1FdePNsO9kbu0kSuxG4FNlfi+qIZueovp67cO15c4v62+vTy3bOq5sb1t5T7cCC2rtb6e67vE/ynuaWWs7u2y/Pl01xPuZyQgK7PL6XeX35ymqMxnLa20foPogerL83nRjge3k1NiEfXkP9uUDvaezS/+7ierGwxf7WhossMvr46l+D7cA/0510+NPlbZfohqBZitwWbvjzSQvsKMcBEmSJEkN8BpsSZIkqUEW2Jp0IuJXYsfH7r44jXduOysifnuA9zSWN0dK0pixL1cn8xIRSZIkqUFTh15kYjn44INz5syZI1rnmWeeYd999x16wVFkDp2TQ6fkYQ4TO4c777zzicwcrSG0JrWd6edh4v4umYM5mEPn5jBgXz/ed1k2PR177LE5UrfccsuI12maOXRODpmdkYc5TOwcgDuyA/rE3XHamX4+c+L+LpmDOZhD5+YwUF/vNdiSJElSgyywJUmSpAZZYEuSJEkNssCWJEmSGmSBLUmSJDXIAluSJElqkAW2JEmS1CALbKlm5tIbmLn0hvFOQ5I0yu7dsG28U9BuzAJbkiRJapAFtiRJktQgC2xJkiSpQRbYkiRJUoMssCVJkqQGWWBLkiRJDbLAliRJkho0ZIEdEXtHxG0R8b2IWBcRf1rih0XErRHRGxHXRMSeJb5Xed1b2mfWtvXREn8wIk6uxeeWWG9ELK3F2+5DkiRJ6lTDOYP9HHBCZr4JOBqYGxFzgEuASzPzcGArsKgsvwjYWuKXluWIiCOA+cCRwFzgMxExJSKmAJcDpwBHAGeVZRlkH5IkSVJHGrLAzsr28nKPMiVwAnBdia8ATivz88prSvuJERElvjIzn8vMHwK9wHFl6s3MhzPzeWAlMK+sM9A+JEmSpI40rGuwy5nmu4FNwBrgB8CTmdlXFlkPTC/z04FHAUr7NuCgerxlnYHiBw2yD0mSJKkjTR3OQpn5AnB0RBwAfAV4w2gmNVIRsRhYDNDV1UVPT8+I1t++ffuI12maOXRGDkuO6uuIPMzBHMZSRBwKXA10UX1DuSwz/yYiLgDeB/x7WfRjmbm6rPNRqsv2XgB+PzNvKvG5wN8AU4B/yMyLS/wwqm8oDwLuBN6Vmc9HxF5l38cCm4HfysxHRv1NS9IoGlaB3S8zn4yIW4BfAg6IiKnlDPMMYENZbANwKLA+IqYC+1N1mv3xfvV12sU3D7KP1ryWAcsAZs+end3d3SN5W/T09DDSdZpmDp2Rw9lLbwDgqrn7TvpjYQ6dlcMo6wOWZOZdEfFq4M6IWFPaLs3Mv6ov3HJPzeuBr0XEz5Xmy4F3UH3reHtErMrM+3npnpqVEfH3VMX5FdTu24mI+WW53xrVdytJo2w4o4i8ppy5JiL2oeo4HwBuAU4viy0Eri/zq8prSvvXMzNLfH4ZZeQwYBZwG3A7MKuMGLInVae9qqwz0D4kSQ3JzI2ZeVeZf5qqjx/skrwm76kZ6L4dSZqwhnMN9iHALRFxD1UxvCYz/wn4CPDhiOil+srvyrL8lcBBJf5hYClAZq4DrgXuB74KnJuZL5Sz0+cBN1F16teWZRlkH5KkUVCGVn0zcGsJnRcR90TE8oiYVmJN3lMz0H07kjRhDXmJSGbeQ9XZtsYfpjpb0Rr/MXDGANu6CLioTXw1sHq4+5AkNS8iXgV8CfhQZj4VEVcAF1Jdl30h8CngPeOU2y7dawOdcS29OXRODl37MO45dMJxMIfRyWFE12BLknZPEbEHVXH9hcz8MkBmPl5r/yzwT+Vlk/fUDHTfzg529V4b6Ixr6c2hc3L42y9cz5keB3MYpRx8VLokTXLlmucrgQcy89O1+CG1xd4J3Ffmm7ynZqD7diRpwvIMtiTprcC7gHvLMw8APkb1ZN2jqS4ReQR4P1T31ERE/z01fZR7agAiov+eminA8pZ7alZGxCeB77LjfTufK/fabKEqyiVpQrPAlqRJLjO/DbQbueNl98bU1mnknprB7tuRpInKS0QkSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUoCEL7Ig4NCJuiYj7I2JdRHywxC+IiA0RcXeZTq2t89GI6I2IByPi5Fp8bon1RsTSWvywiLi1xK+JiD1LfK/yure0z2z03UuSJEkNG84Z7D5gSWYeAcwBzo2II0rbpZl5dJlWA5S2+cCRwFzgMxExJSKmAJcDpwBHAGfVtnNJ2dbhwFZgUYkvAraW+KVlOUmSJKljDVlgZ+bGzLyrzD8NPABMH2SVecDKzHwuM38I9ALHlak3Mx/OzOeBlcC8iAjgBOC6sv4K4LTatlaU+euAE8vykiRJUkeaOpKFyyUabwZuBd4KnBcRC4A7qM5yb6UqvtfWVlvPSwX5oy3x44GDgCczs6/N8tP718nMvojYVpZ/oiWvxcBigK6uLnp6ekbytti+ffuI12maOXRGDkuO6uuIPMzBHCRJE9ewC+yIeBXwJeBDmflURFwBXAhk+fkp4D2jkuUQMnMZsAxg9uzZ2d3dPaL1e3p6GOk6TTOHzsjh7KU3AHDV3H0n/bEwh87KQZI0cQxrFJGI2IOquP5CZn4ZIDMfz8wXMvMnwGepLgEB2AAcWlt9RokNFN8MHBARU1viO2yrtO9flpckSZI60nBGEQngSuCBzPx0LX5IbbF3AveV+VXA/DICyGHALOA24HZgVhkxZE+qGyFXZWYCtwCnl/UXAtfXtrWwzJ8OfL0sL0mStFNmlm8rpdEynEtE3gq8C7g3Iu4usY9RjQJyNNUlIo8A7wfIzHURcS1wP9UIJOdm5gsAEXEecBMwBViemevK9j4CrIyITwLfpSroKT8/FxG9wBaqolySJEnqWEMW2Jn5baDdyB2rB1nnIuCiNvHV7dbLzId56RKTevzHwBlD5ShJkiR1Cp/kKEka7KFiB0bEmoh4qPycVuIREZeVB4HdExHH1La1sCz/UEQsrMWPjYh7yzqX9Q+7OtA+JGmissCWJMHADxVbCtycmbOAm8trqB4aNqtMi4EroCqWgfOphmE9Dji/VjBfAbyvtt7cEh9oH5I0IVlgS5IGe6hY/YFfrQ8Cuzora6lGgzoEOBlYk5lbyrMR1gBzS9t+mbm23Kx+Ne0fKlbfhyRNSCN60IwkaffX8lCxrszcWJoeA7rK/IsPAiv6HxI2WHx9mziD7KOe0y49UAw644FB5tAZOSw5qo+ufZj0x8EcRi8HC2xJ0ovaPFTsxbbMzIgY1aFSB9rHrj5QDDrjgUHm0Bk5nL30BpYc1ceZk/w4mMPo5eAlIpIkoP1DxYDH+597UH5uKvGRPlRsQ5lvjQ+2D0makCywJUkDPlSMHR/41fogsAVlNJE5wLZymcdNwEkRMa3c3HgScFNpeyoi5pR9LaD9Q8Xq+5CkCclLRCRJMPBDxS4Gro2IRcCPgDNL22rgVKAXeBZ4N0BmbomIC6me3gvwiczcUubPAa4C9gFuLBOD7EOSJiQLbEnSYA8VAzixzfIJnDvAtpYDy9vE7wDe2Ca+ud0+JGmi8hIRSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSZIkqUFDFtgRcWhE3BIR90fEuoj4YIkfGBFrIuKh8nNaiUdEXBYRvRFxT0QcU9vWwrL8QxGxsBY/NiLuLetcFhEx2D4kSZKkTjWcM9h9wJLMPAKYA5wbEUcAS4GbM3MWcHN5DXAKMKtMi4EroCqWgfOB44HjgPNrBfMVwPtq680t8YH2IUmSJHWkIQvszNyYmXeV+aeBB4DpwDxgRVlsBXBamZ8HXJ2VtcABEXEIcDKwJjO3ZOZWYA0wt7Ttl5lrMzOBq1u21W4fkiRJUkeaOpKFI2Im8GbgVqArMzeWpseArjI/HXi0ttr6Ehssvr5NnEH20ZrXYqqz5XR1ddHT0zOSt8X27dtHvE7TzKEzclhyVF9H5GEO5iBJmriGXWBHxKuALwEfysynymXSAGRmRkSOQn7D2kdmLgOWAcyePTu7u7tHtO2enh5Guk7TzKEzcjh76Q0AXDV330l/LMyhs3KQJE0cwxpFJCL2oCquv5CZXy7hx8vlHZSfm0p8A3BobfUZJTZYfEab+GD7kCRJkjrScEYRCeBK4IHM/HStaRXQPxLIQuD6WnxBGU1kDrCtXOZxE3BSREwrNzeeBNxU2p6KiDllXwtattVuH5IkSVJHGs4lIm8F3gXcGxF3l9jHgIuBayNiEfAj4MzStho4FegFngXeDZCZWyLiQuD2stwnMnNLmT8HuArYB7ixTAyyD0mSJKkjDVlgZ+a3gRig+cQ2yydw7gDbWg4sbxO/A3hjm/jmdvuQJEmSOpVPcpQkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSRIRsTwiNkXEfbXYBRGxISLuLtOptbaPRkRvRDwYESfX4nNLrDciltbih0XErSV+TUTsWeJ7lde9pX3mGL1lSRo1FtiSJKiGSp3bJn5pZh5dptUAEXEEMB84sqzzmYiYEhFTgMuBU4AjgLPKsgCXlG0dDmwFFpX4ImBriV9alpOkCc0CW5JEZn4T2DLkgpV5wMrMfC4zf0j13IPjytSbmQ9n5vPASmBeeYjYCcB1Zf0VwGm1ba0o89cBJ5blJWnCGs6DZiRJk9d5EbEAuANYkplbgenA2toy60sM4NGW+PHAQcCTmdnXZvnp/etkZl9EbCvLP1FPIiIWA4sBurq66OnpGfEb2b59+06t1yRz6IwclhzVR9c+TPrjYA6jl4MFtiRpIFcAFwJZfn4KeM94JJKZy4BlALNnz87u7u4Rb6Onp4edWa9J5tAZOZy99AaWHNXHmZP8OJjD6OXgJSKSpLYy8/HMfCEzfwJ8luoSEIANwKG1RWeU2EDxzcABETG1Jb7Dtkr7/mV5SZqwLLAlSW1FxCG1l+8E+kcYWQXMLyOAHAbMAm4DbgdmlRFD9qS6EXJVZiZwC3B6WX8hcH1tWwvL/OnA18vykjRheYmIJImI+CLQDRwcEeuB84HuiDia6hKRR4D3A2Tmuoi4Frgf6APOzcwXynbOA24CpgDLM3Nd2cVHgJUR8Ungu8CVJX4l8LmI6KW6yXL+6L5TSRp9FtiSJDLzrDbhK9vE+pe/CLioTXw1sLpN/GFeusSkHv8xcMaIkpWkDuclIpIkSVKDLLClNu7dsG28U5AkSROUBbYkSZLUIAtsSZIkqUEW2JIkSVKDhiywI2J5RGyKiPtqsQsiYkNE3F2mU2ttH42I3oh4MCJOrsXnllhvRCytxQ+LiFtL/JoydiplfNVrSvzWiJjZ2LuWJEmSRslwzmBfBcxtE780M48u02qAiDiCagzTI8s6n4mIKRExBbgcOAU4AjirLAtwSdnW4cBWYFGJLwK2lvilZTlJkiSpow1ZYGfmN6kG/x+OecDKzHwuM38I9FKNe3oc0JuZD2fm88BKYF5EBHACcF1ZfwVwWm1bK8r8dcCJZXlJkiSpY+3Kg2bOi4gFwB3AkszcCkwH1taWWV9iAI+2xI8HDgKezMy+NstP718nM/siYltZ/onWRCJiMbAYoKuri56enhG9ke3bt494naaZQ2fksOSo6lexax8m/bEwh87KQZI0cexsgX0FcCHV43MvBD4FvKeppEYqM5cBywBmz56d3d3dI1q/p6eHka7TNHPojBzOXnoDUBXaZ07yY2EOnZWDJGni2KlRRDLz8cx8ITN/AnyWlx5/uwE4tLbojBIbKL4ZOCAiprbEd9hWad+/LC9JkiR1rJ0qsCPikNrLdwL9I4ysAuaXEUAOA2YBtwG3A7PKiCF7Ut0IuSozE7gFOL2svxC4vrathWX+dODrZXlJkiSpYw15iUhEfBHoBg6OiPXA+UB3RBxNdYnII8D7ATJzXURcC9wP9AHnZuYLZTvnATcBU4Dlmbmu7OIjwMqI+CTwXeDKEr8S+FxE9FLdZDl/V9+sJEmSNNqGLLAz86w24SvbxPqXvwi4qE18NbC6TfxhXrrEpB7/MXDGUPlJkiRJncQnOUqSJEkNssCWJEmSGmSBLUmSJDXIAluSJElqkAW2JEmS1CALbEmSJKlBFtiSJElSgyywJUmSpAZZYEuSiIjlEbEpIu6rxQ6MiDUR8VD5Oa3EIyIui4jeiLgnIo6prbOwLP9QRCysxY+NiHvLOpdFRAy2D0mayCywJUkAVwFzW2JLgZszcxZwc3kNcAowq0yLgSugKpaB84HjqZ7Qe36tYL4CeF9tvblD7EOSJiwLbEkSmflNYEtLeB6wosyvAE6rxa/OylrggIg4BDgZWJOZWzJzK7AGmFva9svMtZmZwNUt22q3D0masCywJUkD6crMjWX+MaCrzE8HHq0tt77EBouvbxMfbB+SNGFNHe8EJEmdLzMzInK89hERi6kuR6Grq4uenp4Rb3/79u07tV6TzKEzclhyVB9d+zDpj4M5jF4OFtiSpIE8HhGHZObGcpnHphLfABxaW25GiW0AulviPSU+o83yg+1jB5m5DFgGMHv27Ozu7m632KB6enrYmfWaZA6dkcPZS29gyVF9nDnJj4M5jF4OXiIiSRrIKqB/JJCFwPW1+IIymsgcYFu5zOMm4KSImFZubjwJuKm0PRURc8roIQtattVuH5I0YXkGW5JERHyR6uzzwRGxnmo0kIuBayNiEfAj4Myy+GrgVKAXeBZ4N0BmbomIC4Hby3KfyMz+GyfPoRqpZB/gxjIxyD4kacKywJYkkZlnDdB0YptlEzh3gO0sB5a3id8BvLFNfHO7fUjSRDbkJSI+fECSJEkavuFcg30VPnxAkiRJGpYhC2wfPiBJkiQN386OIuLDByRJkqQ2dvkmx/F++ADs+gMIdscBzs1h5yw5qg/ABxCYQ8flIEmaOHa2wO6Yhw/Arj+AYHcc4Nwcds7ZS28A8AEE5tBxOUiSJo6dvUTEhw9IkiRJbQx5BtuHD0iSJEnDN2SB7cMHJEmSpOHb2UtEJEmSJLVhgS1JkiQ1yAJbkiRJapAFtiRJktQgC2xJkiSpQRbYkiRJUoMssCVJkqQGWWBLkiRJDbLAliRJkhpkgS1JkiQ1yAJbkiRJapAFtiRJktQgC2xJkiSpQRbYkiRJUoMssCVJg4qIRyLi3oi4OyLuKLEDI2JNRDxUfk4r8YiIyyKiNyLuiYhjattZWJZ/KCIW1uLHlu33lnVj7N+lJDXHAluSNBy/mplHZ+bs8nopcHNmzgJuLq8BTgFmlWkxcAVUBTlwPnA8cBxwfn9RXpZ5X229uaP/diRp9FhgS5J2xjxgRZlfAZxWi1+dlbXAARFxCHAysCYzt2TmVmANMLe07ZeZazMzgatr25KkCWnqeCcgSep4CfxzRCTwPzNzGdCVmRtL+2NAV5mfDjxaW3d9iQ0WX98mvoOIWEx1Rpyuri56enpG/Ca2b9++U+s1yRw6I4clR/XRtQ+T/jiYw+jlsEsFdkQ8AjwNvAD0Zebs8jXgNcBM4BHgzMzcWq6p+xvgVOBZ4OzMvKtsZyHwR2Wzn8zMFSV+LHAVsA+wGvhgOcMhSRo7b8vMDRHxWmBNRHy/3piZWYrvUVOK+mUAs2fPzu7u7hFvo6enh51Zr0nm0Bk5nL30BpYc1ceZk/w4mMPo5dDEJSJelydJu7HM3FB+bgK+QtVXP14u76D83FQW3wAcWlt9RokNFp/RJi5JE9ZoXIPtdXmStJuIiH0j4tX988BJwH3AKqB/JJCFwPVlfhWwoIwmMgfYVi4luQk4KSKmlZMoJwE3lbanImJO+aZzQW1bkjQh7eo12ON+XR7s+rV5u+O1P+awc5Yc1QfgtXnm0HE5jKMu4Ctl5LypwP/OzK9GxO3AtRGxCPgRcGZZfjXVpYC9VJcDvhsgM7dExIXA7WW5T2TmljJ/Di9dDnhjmSRpwtrVAnvcr8sr+9mla/N2x2t/zGHnnL30BgCvzTOHjsthvGTmw8Cb2sQ3Aye2iSdw7gDbWg4sbxO/A3jjLicrSR1ily4R8bo8SZIkaUc7XWB7XZ4kSZL0crtyiYjX5UmSJEktdrrA9ro8SZIk6eV8VLokSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSZIkqUEdX2BHxNyIeDAieiNi6XjnI0lqnn29pN1JRxfYETEFuBw4BTgCOCsijhjfrCRJTbKvl7S76egCGzgO6M3MhzPzeWAlMG+cc5IkNcu+XtJuZep4JzCE6cCjtdfrgeNbF4qIxcDi8nJ7RDw4wv0cDDyxUxk2xxw6Jwd+Hw7+/d8Z9zw64ViYw87n8NOjkchuasi+voF+Hibu75I5jAL7eXNoKIe2fX2nF9jDkpnLgGU7u35E3JGZsxtMyRwmcA6dkoc5mINesqv9PHTGv6M5mIM5TI4cOv0SkQ3AobXXM0pMkrT7sK+XtFvp9AL7dmBWRBwWEXsC84FV45yTJKlZ9vWSdisdfYlIZvZFxHnATcAUYHlmrhuFXe3S144NMYdKJ+QAnZGHOVTMYTdnXz/mzKFiDhVzqDSaQ2Rmk9uTJEmSJrVOv0REkiRJmlAssCVJkqQGTeoCOyKOjoi1EXF3RNwREceVeETEZeWRvfdExDGjnMfvRcT3I2JdRPxFLf7RksODEXHyaOZQ9rckIjIiDi6vx+w4RMRflmNwT0R8JSIOqLWN2XEYj8c1R8ShEXFLRNxffgc+WOIHRsSaiHio/Jw2BrlMiYjvRsQ/ldeHRcSt5XhcU25AG839HxAR15XfhQci4pfG+jhExP8o/w73RcQXI2LvsT4Oao79/MvyGLd+vuxv3Pv68ejny347oq8f736+7HP37+szc9JOwD8Dp5T5U4Ge2vyNQABzgFtHMYdfBb4G7FVev7b8PAL4HrAXcBjwA2DKKOZxKNUNRj8CDh6H43ASMLXMXwJcMtbHgermqh8APwPsWfZ7xBj8Hh4CHFPmXw38S3nffwEsLfGl/cdklHP5MPC/gX8qr68F5pf5vwd+d5T3vwJ4b5nfEzhgLI8D1QNPfgjsU3v/Z4/1cXBq9N/Ufv6lPMa1ny/7G9e+frz6+bLvjujrx7ufL/vZ7fv6SX0GG0hgvzK/P/BvZX4ecHVW1gIHRMQho5TD7wIXZ+ZzAJm5qZbDysx8LjN/CPRSPU54tFwK/L9Ux6TfmB2HzPznzOwrL9dSjYPbn8NYHYdxeVxzZm7MzLvK/NPAA1R//POoOiHKz9NGM4+ImAH8N+AfyusATgCuG4scImJ/4L8AVwJk5vOZ+SRjfByoRlfaJyKmAq8ENjKGx0GNs59/ybj289ARff249PPQGX39ePfzZZ+Toq+f7AX2h4C/jIhHgb8CPlri7R7bO32Ucvg54FfKVxLfiIi3jHUOETEP2JCZ32tpGsvjUPceqjMqY53DeL3fF0XETODNwK1AV2ZuLE2PAV2jvPu/pvrw/Ul5fRDwZO3DcLSPx2HAvwP/q3x9+Q8RsS9jeBwycwNVX/CvVJ3tNuBOxvY4qFkfwn6+E/t5GJ++ftz7eRjXvv6vGd9+HiZJX9/R42A3ISK+BryuTdPHgROB/5GZX4qIM6n+N/X2Mc5hKnAg1VdzbwGujYifGeMcPkb1td2oGiyHzLy+LPNxoA/4wmjn02ki4lXAl4APZeZT1YmFSmZmRIzamJoR8WvApsy8MyK6R2s/Q5gKHAP8XmbeGhF/Q/U14YvG4DhMozqLchjwJPCPwNzR2p+aYT8/rBzGpJ8fKg/7+vHr6zukn4dJ0tfv9gV2Zg7YkUbE1cAHy8t/pHxlQsOP7R0ih98FvpzVBT+3RcRPgIPHKoeIOIrqF+x75Y98BnBXVDcCjdlxKLmcDfwacGI5HjSdwxDG7XHNEbEHVYf7hcz8cgk/HhGHZObG8pXtpoG3sMveCvx6RJwK7E31lfrfUH1dPLX8j360j8d6YH1m3lpeX0fV6Y7lcXg78MPM/HeAiPgy1bEZy+OgEbKfHzyHseznB8ujls/ZjF9fP279PIx7X98J/TxMkr5+sl8i8m/Afy3zJwAPlflVwIKozAG21b62aNr/oboBhoj4OaqL/Z8oOcyPiL0i4jBgFnBb0zvPzHsz87WZOTMzZ1L94h+TmY8xhschIuZSfW3165n5bK1pTI5DMS6Pay7XwF0JPJCZn641rQIWlvmFwPWjlUNmfjQzZ5TfgfnA1zPzt4FbgNPHKIfHgEcj4udL6ETgfsbwOFB9XTgnIl5Z/l36cxiz46DG2c93SD8PHdHXj0s/D+Pf13dCP1/ymBx9fY7BnbOdOgFvo7rm5ntU10EdW+IBXE51p/G9wOxRzGFP4PPAfcBdwAm1to+XHB6k3AU/BsfkEV66u3wsj0Mv1XVxd5fp78fjOFDdUf8vZX8fH8PfwwTuqb3/U6mujbuZqiD4GnDgGOXTzUt3l/8M1YdcL9XZv71Ged9HA3eUY/F/gGljfRyAPwW+X/4mP0c1qsGYHgenRv897edfns+49PNlf+Pe149HP1/7XeyIvn48+/myz92+r/dR6ZIkSVKDJvslIpIkSVKjLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSbskIrojYv145yFJGj8RkRFx+Hjn0SkssHczEfH5iNgYEU9FxL9ExHtrbe+NiN6I2B4RX42I19faDoiIFRGxqUwXtGz3lyPitoh4OiLuiYi31dp+NSLujYgnI2JzRHwlIqbX2q+KiOfLfvunKbX2V0bEZyLiiYjYFhHfrLVFRFxStru5zEeb972g/HHX3++NLft8PiLuHcYxzIh4pqyzISI+3ZLvXhGxvBzjxyLiw7W2IyLijojYWqavRcQRLds/LiJWl+O1pRzXd9faT4yI70fEsxFxS0T8dK3tzIj4TmnrGeq9jAY7UWn02Zfbl3eyiOip/xupjcx02o0m4EhgrzL/BuAx4FigG9hU2vcErgC+UVvvfwH/CLwSmAn8AHh3aTsQ2AycAUwBfgfYCkwr7V3A68v8XsBfAKtq274K+OQgOX8eWAm8pmz/2Frb+4EHgRnAdOB+4AMt608Dvg/cB7x3kP30AH8yjGOYwOFl/nBgA/C+WvufA98q+/2FcoznlrYDyvGL8l5+H7intu4vAduBjwAHl+WOBa4t7QcD28qx3hv4S2Btbf23A2cCfwL0jNPv2IvHp7zuBtaP9+++k9PuNNmX25d38lT+Dd7bEtvhs2GyT+OegNMo/uPCzwMbyx/xXwGX19peX/4Yfra8fgJ4S639Y8C3yvyvAetatv0vwKI2+9yrdFr312IDdspUHxxPAfsN0P4dYHHt9aJ6J1Vifw+c0+4PvrbMTOAFYOYwjltrAXlty7H7N+Ck2usLgZVttjMVOBd4thb7dn1bbdZZDHyn9npf4D+AN7Qs997hdsrlvSfwbuBRqg/UDwBvAe4BngT+rmWd9wAPlGVvAn66xL9ZtvVM+XD5LUqBDSyh+uDfSPlAd3Jy2vXJvnyHZSZzX95Ny8kM4BHg7WX+gvIerwaeBtYBs1uW/cPS7z8DXEn1n6oby/Jfo/xnqyw/p/y7PQl8D+gu8YvKv8GPy+fA39WO9weAh8o6lwMx3n8/4zV5ichuqHxF9yzVmYCNwOr+pvpi5ecb28T65wdqe1l7RPxURDxJ1YH8AdWZj7pzyldod0bEb9bixwE/Av60fK14b0v7kVR/2P2+V2L9+z0OmE3VMQ9mAdWHzCNDLLeDiHgD8CtAb3k9DThksJzKck9SdT5/C/xZib2S6qzHdYPscof3m5nPUJ2BOnLANYbveGAWVVH818DHqc6iHAmcGRH/teQ5j+pD+TeozkR9C/hiyee/lG29KTNflZnXlNevA/anOjO1CLi8HCtJO8m+vC378sH9OtW3CAcAq4C/a2n/TeAdwM8B/52quP4YVV//Cqoz9ZRLg24APkn1zccfAF+KiNdk5sepPhfOK58D59W2/2tUJ29+keo/hCc3/xYnBgvs3VBmngO8mqoz+TLwHPBVqiLqFyNiH6qvpZLqa0RK+9KIeHVU19e+p9b2/wGvj4izImKPiFgI/Gytncz818w8gOprsT+i+kDodxlVYfda4I+BqyLiraVtBlXnvo3qTMx5wIqI+IXS/qrS1m8b8KpyPd8U4DNUf+Q/GeKwLKA6+zJcd0XEM1RncXvKfvrz6c+jntOr6yuXY7F/eT/fLeFpVH9zGwfZb+v7bbv9nXRhZv44M/+Z6uzFFzNzU2ZuoOos31yW+wDw55n5QGb2UX2oHF2/frCN/wQ+kZn/mZmrqc5q/HwDOUuTln15W/blg/t2Zq7OzBeAzwFvamn/28x8vNbv35qZ383MHwNf4aXPgd8BVpdt/SQz1wB3AKcOsf+LM/PJzPxX4Bbg6Ibe14Rjgb2byswXMvPbVJ3e72bm14DzgS9RfU30CNVXQv2jP/w+1RmLh4Drqc5Yri/b2gzMAz4MPA7Mpfoq6WUjR2TmFmAFcH1ETC2xuzJzc2b2leLrC1RnRyn7/E+qrx2fz8xvUP1RnlTatwP71XaxH7A9q++jzqG6Jm7tYMciqpt4XsfgZxtaHUPVQf4W1ZnffWv59OdRz+np1g2UMxZ/D1wdEa+lutziJ1RnTQbS+n4H3P5OeLw2/x9tXvd/4Pw08Dflxp0ngS1UZ7mmM7DNpRjv92xte5J2kn35S+zLh+Wx2vyzwN79/37FSD4Hzuj/HCifBW9j8Pfcbv+T9nPAAnv3N5XqDAWZeXlmzsrMLqrOeSrVzSRk5pbM/O3MfF1mHkn1u3Fb/0Yy8xuZ+ZbMPBB4F9X1drfR3lSqMxytncuLm+OlrynvGaC93zp2/B/4m0oM4ETgneXu78eAXwY+FRGtX4ktBL6cmdsZgaxcS3XW509KbCvVWYuBcmr1CqqzQ9Mz89myrd8cYFloeb8RsS/Vv99A2x8NjwLvz8wDatM+mfmdMcxB0o7sy+3Ln6H2bUM58/+aXdjeYB4FPtfyObBvZl5c2nOwlYU3Oe5OE1VHOJ/qf4xTqK59eobqmqy9qb6+C+CnqL4q+7Pauj8LHFTWO4XqRpkja+1vBvag6mj/Gvi/tbbfoLoc4BVUf+zXAnfV2k8vOb2C6mzG07x0s8QeVNfE/TFVZ/7W0v6G0v4Bqq/2plN97biOcuc51TVmr6tN36E6M7N/bd/7UH0td8IIjmPrjTFHleP4uvL6YuAbVF8TvoGqk+6/8/wd5VhNKcfqMqobafYu7b9MdWbjD4GDSuxNlBtryvHbRtVx7w1cwo53nk8p8Q9Q3XC4N7DHEO9nZnlPU2ux9f3/BuX154E/KvPvpPqwPrK83h84o7bsY+x4Y1A3g9x44+TkNLIJ+3L78vbvZ3+qs8L/rRzv84E+drzJ8fO15WdS6/tp6Zep+v0Laq/fC3ytzB9K1defXMu1G5hR2ldS+70b4HhfxSCjzuzu07gn4NTgP2b1B/0Nqrt3nwLupQxJVDqw/juHH6O6O3xKbd0zS+fxLHA3cHLLtr9YOottwDXAa2ttvwf8sLbtlZRRJ0r7t8p6T1Hd9DG/ZdtHUp0NeIZq6KZ31tqC6iabLWX6Cwa4K5n2wwadRXXjzbDvZG7tJErsRuBTZX4vYHl5P48DH64tdwbVNYvbgX+nuknkF1u2dVzZ3rbynm4FFtTa31628R/lPc2stZ1d8qtPVw3xfmYyggK7vH5X+f15iupMxvJa2weoPoieLL833VhgOzk1NmFf3oN9+UDv6ezS/26iuvHwxb6WBgvs8vp4qt/DLbVj8FOl7ZeoRqDZClzW7ngzyQvsKAdBkiRJUgO8BluSJElqkAW2Jp2I+JXY8bG7L07jndvOiojfHuA9jeXNkZI0ZuzL1cm8RESSJElqkGewJUmSpAZNHXqRieXggw/OmTNnjmidZ555hn333XfoBUeROXRODp2ShzlM7BzuvPPOJzJztMaondR2pp+Hifu7ZA7mYA6dm8OAff14D2PS9HTsscfmSN1yyy0jXqdp5tA5OWR2Rh7mMLFzAO7IDugTd8dpZ/r5zIn7u2QO5mAOnZvDQH29l4hIkiRJDbLAliRJkhpkgS1JkiQ1yAJbkiRJapAFtiRJktQgC2xJkiSpQRbYUs3MpTcwc+kN452GJGmU3bth23inoN2YBbYkSZLUIAtsSZIkqUEW2JI0yUXEoRFxS0TcHxHrIuKDJX5BRGyIiLvLdGptnY9GRG9EPBgRJ9fic0usNyKW1uKHRcStJX5NROxZ4nuV172lfeYYvnVJGhUW2JKkPmBJZh4BzAHOjYgjStulmXl0mVYDlLb5wJHAXOAzETElIqYAlwOnAEcAZ9W2c0nZ1uHAVmBRiS8Ctpb4pWU5SZrQLLAlaZLLzI2ZeVeZfxp4AJg+yCrzgJWZ+Vxm/hDoBY4rU29mPpyZzwMrgXkREcAJwHVl/RXAabVtrSjz1wEnluUlacKaOt4JSJI6R7lE483ArcBbgfMiYgFwB9VZ7q1Uxffa2mrreakgf7QlfjxwEPBkZva1WX56/zqZ2RcR28ryT7TktRhYDNDV1UVPT8+I39v27dt3ar0mmUPn5NC1D+OeQyccB3MYnRwssCVJAETEq4AvAR/KzKci4grgQiDLz08B7xmP3DJzGbAMYPbs2dnd3T3ibfT09LAz6zXJHDonh7/9wvWc6XEwh1HKwUtEJElExB5UxfUXMvPLAJn5eGa+kJk/AT5LdQkIwAbg0NrqM0psoPhm4ICImNoS32FbpX3/srwkTVgW2JI0yZVrnq8EHsjMT9fih9QWeydwX5lfBcwvI4AcBswCbgNuB2aVEUP2pLoRclVmJnALcHpZfyFwfW1bC8v86cDXy/KSNGF5iYgk6a3Au4B7I+LuEvsY1SggR1NdIvII8H6AzFwXEdcC91ONQHJuZr4AEBHnATcBU4DlmbmubO8jwMqI+CTwXaqCnvLzcxHRC2yhKsolaUKzwJakSS4zvw20G7lj9SDrXARc1Ca+ut16mfkwL11iUo//GDhjJPlKUqcb8hKRiNg7Im6LiO+VBxD8aYmP+KEBTT2YQJIkSepUw7kG+znghMx8E3A0MDci5jDChwY0/GACSZIkqSMNWWBnZXt5uUeZkpE/NKDJBxNIkiRJHWlYo4iUM813A5uANcAPGOZDA4D+hwa8GG9ZZ6D4YA8mkCRJkjrSsG5yLHeHHx0RBwBfAd4wmkmN1K4+4Wt3fIKQOeycJUf1dUQe5mAOkqSJa0SjiGTmkxFxC/BLlIcGlDPM7R4asL7loQEDPYCAAeIvPpigzT5a89qlJ3ztjk8QMoedc/bSGwC4au6+k/5YmENn5SBJmjiGM4rIa8qZayJiH+AdwAOM/KEBTT6YQJIkSepIwzmDfQiwooz28Qrg2sz8p4i4nxE8NKDhBxNIkiRJHWnIAjsz7wHe3CY+4ocGNPVgAkmSJKlTDWsUEUmSJEnDY4EtSZImlZnlhnZptFhgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSRIRcWhE3BIR90fEuoj4YIkfGBFrIuKh8nNaiUdEXBYRvRFxT0QcU9vWwrL8QxGxsBY/NiLuLetcFhEx2D4kaaKywJYkAfQBSzLzCGAOcG5EHAEsBW7OzFnAzeU1wCnArDItBq6AqlgGzgeOB44Dzq8VzFcA76utN7fEB9qHJE1IFtiSJDJzY2beVeafBh4ApgPzgBVlsRXAaWV+HnB1VtYCB0TEIcDJwJrM3JKZW4E1wNzStl9mrs3MBK5u2Va7fUjShDRkgT3I14YXRMSGiLi7TKfW1vlo+QrwwYg4uRafW2K9EbG0Fj8sIm4t8WsiYs8S36u87i3tMxt995Kklyl97ZuBW4GuzNxYmh4Dusr8dODR2mrrS2yw+Po2cQbZhyRNSFOHsUz/14Z3RcSrgTsjYk1puzQz/6q+cPlKcT5wJPB64GsR8XOl+XLgHVQd6+0RsSoz7wcuKdtaGRF/Dyyi+ipxEbA1Mw+PiPllud/alTcsSRpYRLwK+BLwocx8qlwmDUBmZkTkaO5/oH1ExGKqS1Ho6uqip6dnxNvevn37Tq3XJHPojByWHNVH1z5M+uNgDqOXw5AFdjmrsLHMPx0R/V8bDmQesDIznwN+GBG9VNfhAfRm5sMAEbESmFe2dwLw/5RlVgAXUBXY88o8wHXA30VElK8XJUkNiog9qIrrL2Tml0v48Yg4JDM3lss8NpX4BuDQ2uozSmwD0N0S7ynxGW2WH2wfL8rMZcAygNmzZ2d3d3frIkPq6elhZ9Zrkjl0Rg5nL72BJUf1ceYkPw7mMHo5DOcM9otavjZ8K3BeRCwA7qA6y72VqvheW1ut/jVg69eGxwMHAU9mZl+b5V/8qjEz+yJiW1n+iZa8dunMxu74Pydz2DlLjurriDzMwRzGWhnR40rggcz8dK1pFbAQuLj8vL4WP6+cLDke2FYK5JuAP6vd2HgS8NHM3BIRT0XEHKrPkAXA3w6xD0makIZdYLf52vAK4EIgy89PAe8ZlSyHsKtnNnbH/zmZw845e+kNAFw1d99JfyzMobNyGANvBd4F3BsRd5fYx6iK3msjYhHwI+DM0rYaOBXoBZ4F3g1QCukLgdvLcp/IzC1l/hzgKmAf4MYyMcg+JGlCGlaB3e5rw8x8vNb+WeCfysuBvjZkgPhmqrvPp5az2PXl+7e1PiKmAvuX5SVJDcrMbwMxQPOJbZZP4NwBtrUcWN4mfgfwxjbxze32IUkT1XBGEWn7tWG5Tq7fO4H7yvwqYH4ZAeQwqrFOb6M6mzGrjBiyJ9WNkKtKJ30LcHpZv/UryP6HFJwOfN3rryVJktTJhnMGe6CvDc+KiKOpLhF5BHg/QGaui4hrgfupRiA5NzNfAIiI84CbgCnA8sxcV7b3EWBlRHwS+C5VQU/5+blyo+QWqqJckiRJ6ljDGUVkoK8NVw+yzkXARW3iq9utV0YWOa5N/MfAGUPlKEmSJHUKn+QoSZIkNcgCW5IkSWqQBbYkSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNWjIAjsiDo2IWyLi/ohYFxEfLPEDI2JNRDxUfk4r8YiIyyKiNyLuiYhjattaWJZ/KCIW1uLHRsS9ZZ3LIiIG24ckSZLUqYZzBrsPWJKZRwBzgHMj4ghgKXBzZs4Cbi6vAU4BZpVpMXAFVMUycD5wPHAccH6tYL4CeF9tvbklPtA+JEmSpI40ZIGdmRsz864y/zTwADAdmAesKIutAE4r8/OAq7OyFjggIg4BTgbWZOaWzNwKrAHmlrb9MnNtZiZwdcu22u1DkiRJ6khTR7JwRMwE3gzcCnRl5sbS9BjQVeanA4/WVltfYoPF17eJM8g+WvNaTHW2nK6uLnp6ekbytti+ffuI12maOXRGDkuO6uuIPMzBHCRJE9ewC+yIeBXwJeBDmflUuUwagMzMiMhRyG9Y+8jMZcAygNmzZ2d3d/eItt3T08NI12maOXRGDmcvvQGAq+buO+mPhTl0Vg6SpIljWKOIRMQeVMX1FzLzyyX8eLm8g/JzU4lvAA6trT6jxAaLz2gTH2wfkqQGRcTyiNgUEffVYhdExIaIuLtMp9baPlpuTH8wIk6uxeeWWG9ELK3FD4uIW0v8mojYs8T3Kq97S/vMMXrLkjRqhjOKSABXAg9k5qdrTauA/pFAFgLX1+ILymgic4Bt5TKPm4CTImJaubnxJOCm0vZURMwp+1rQsq12+5AkNesqXrrBvO7SzDy6TKsByo3u84EjyzqfiYgpETEFuJzqZvcjgLPKsgCXlG0dDmwFFpX4ImBriV9alpOkCW04Z7DfCrwLOKHlLMbFwDsi4iHg7eU1wGrgYaAX+CxwDkBmbgEuBG4v0ydKjLLMP5R1fgDcWOID7UOS1KDM/CawZcgFK/OAlZn5XGb+kKrvPq5MvZn5cGY+D6wE5pWTJycA15X1W2+M77+Z/TrgxP6hWiVpohryGuzM/DYwUGd3YpvlEzh3gG0tB5a3id8BvLFNfHO7fUiSxsx5EbEAuINqyNatVDeir60tU785vfVm9uOBg4AnM7OvzfIv3gCfmX0Rsa0s/0Q9iV29mR0642ZVc+iMHJYc1UfXPkz642AOo5fDiEYRkSRNKldQffOY5eengPeMRyK7ejM7dMbNqubQGTmcvfQGlhzVx5mT/DiYw+jl4KPSJUltZebjmflCZv6E6pK/40rTSG9m30z1TISpLfEdtlXa9y/LS9KEZYEtSWqrfxSn4p1A/wgjq4D5ZQSQw6iewHsb1f01s8qIIXtS3Qi5qlw6eAtwelm/9cb4/pvZTwe+XpaXpAnLS0QkSUTEF4Fu4OCIWA+cD3RHxNFUl4g8ArwfIDPXRcS1wP1AH3BuZr5QtnMe1ahRU4Dlmbmu7OIjwMqI+CTwXarRqSg/PxcRvVQ3Wc4f3XcqSaPPAluSRGae1SZ8ZZtY//IXARe1ia+mGk2qNf4wL11iUo//GDhjRMlKUofzEhFJkiSpQRbYkiRJUoMssCVJkqQGWWBLkiRJDbLAliRJkhpkgS1JkiQ1yAJbkiRJapAFtiRJktQgC2xJkiSpQRbYkiRJUoMssCVJkqQGWWBLkiRJDRqywI6I5RGxKSLuq8UuiIgNEXF3mU6ttX00Inoj4sGIOLkWn1tivRGxtBY/LCJuLfFrImLPEt+rvO4t7TMbe9fSEO7dsG28U5AkSRPUcM5gXwXMbRO/NDOPLtNqgIg4ApgPHFnW+UxETImIKcDlwCnAEcBZZVmAS8q2Dge2AotKfBGwtcQvLctJkiRJHW3IAjszvwlsGeb25gErM/O5zPwh0AscV6bezHw4M58HVgLzIiKAE4DryvorgNNq21pR5q8DTizLS5IkSR1r6i6se15ELADuAJZk5lZgOrC2tsz6EgN4tCV+PHAQ8GRm9rVZfnr/OpnZFxHbyvJPtCYSEYuBxQBdXV309PSM6I1s3759xOs0zRw6I4clR1W/il37MOmPhTl0Vg6SpIljZwvsK4ALgSw/PwW8p6mkRiozlwHLAGbPnp3d3d0jWr+np4eRrtM0c+iMHM5eegNQFdpnTvJjYQ6dlYMkaeLYqVFEMvPxzHwhM38CfJbqEhCADcChtUVnlNhA8c3AARExtSW+w7ZK+/5leUmSJKlj7VSBHRGH1F6+E+gfYWQVML+MAHIYMAu4DbgdmFVGDNmT6kbIVZmZwC3A6WX9hcD1tW0tLPOnA18vy0uSJEkda8hLRCLii0A3cHBErAfOB7oj4miqS0QeAd4PkJnrIuJa4H6gDzg3M18o2zkPuAmYAizPzHVlFx8BVkbEJ4HvAleW+JXA5yKil+omy/m7+mYlSZKk0TZkgZ2ZZ7UJX9km1r/8RcBFbeKrgdVt4g/z0iUm9fiPgTOGyk+SJEnqJD7JUZIkSWqQBbYkSZLUIAtsSZIkqUEW2JIkImJ5RGyKiPtqsQMjYk1EPFR+TivxiIjLIqI3Iu6JiGNq6ywsyz8UEQtr8WMj4t6yzmX9T+YdaB+SNJFZYEuSAK4C5rbElgI3Z+Ys4ObyGuAUqmFYZ1E9RfcKqIplqpGmjqe6ef38WsF8BfC+2npzh9iHJE1YFtiSJDLzm1RDotbNA1aU+RXAabX41VlZS/XAsEOAk4E1mbklM7cCa4C5pW2/zFxbnmdwdcu22u1DkiasnX1UuiRp99eVmRvL/GNAV5mfDjxaW259iQ0WX98mPtg+dhARi6nOltPV1UVPT8+I38z27dt3ar0mmUNn5LDkqD669mHSHwdzGL0cLLAlSUPKzIyIUX2a7mD7yMxlwDKA2bNnZ3d394i339PTw86s1yRz6Iwczl56A0uO6uPMSX4czGH0cvASEUnSQB4vl3dQfm4q8Q3AobXlZpTYYPEZbeKD7UOSJiwLbEnSQFYB/SOBLASur8UXlNFE5gDbymUeNwEnRcS0cnPjScBNpe2piJhTRg9Z0LKtdvuQpAnLS0QkSUTEF4Fu4OCIWE81GsjFwLURsQj4EXBmWXw1cCrQCzwLvBsgM7dExIXA7WW5T2Rm/42T51CNVLIPcGOZGGQfkjRhWWBLksjMswZoOrHNsgmcO8B2lgPL28TvAN7YJr653T4kaSLzEhFJkiSpQRbYkiRJUoMssCVJkqQGWWBLkiRJDbLAliRJkho0ZIEdEcsjYlNE3FeLHRgRayLiofJzWolHRFwWEb0RcU9EHFNbZ2FZ/qGIWFiLHxsR95Z1LitjpA64D0mSJKmTDecM9lXA3JbYUuDmzJwF3FxeA5wCzCrTYuAKqIplqjFVjweOA86vFcxXAO+rrTd3iH1IkiRJHWvIAjszvwlsaQnPA1aU+RXAabX41VlZCxxQHn17MrAmM7dk5lZgDTC3tO2XmWvLuKpXt2yr3T4kSZKkjrWzD5rpKo++BXgM6Crz04FHa8utL7HB4uvbxAfbx8tExGKqM+Z0dXXR09Mzojezffv2Ea/TNHPojByWHNUHQNc+TPpjYQ6dlYMkaeLY5Sc5ZmZGRDaRzM7uIzOXAcsAZs+end3d3SPafk9PDyNdp2nm0Bk5nL30BqAqtM+c5MfCHDorB0nSxLGzo4g8Xi7voPzcVOIbgENry80oscHiM9rEB9uHJEmS1LF2tsBeBfSPBLIQuL4WX1BGE5kDbCuXedwEnBQR08rNjScBN5W2pyJiThk9ZEHLttrtQ5IkSepYQ14iEhFfBLqBgyNiPdVoIBcD10bEIuBHwJll8dXAqUAv8CzwboDM3BIRFwK3l+U+kZn9N06eQzVSyT7AjWVikH1IkiRJHWvIAjszzxqg6cQ2yyZw7gDbWQ4sbxO/A3hjm/jmdvuQJEmSOplPcpQkSZIaZIEtSZIkNcgCW5IkSWqQBbYkSZLUIAtsSZIkqUEW2JIkSVKDLLAlSZKkBllgS5IkSQ2ywJYkSZIaZIEtSZIkNcgCW5I0qIh4JCLujYi7I+KOEjswItZExEPl57QSj4i4LCJ6I+KeiDimtp2FZfmHImJhLX5s2X5vWTfG/l1KUnMssCVJw/GrmXl0Zs4ur5cCN2fmLODm8hrgFGBWmRYDV0BVkAPnA8cDxwHn9xflZZn31dabO/pvR5JGjwW2JGlnzANWlPkVwGm1+NVZWQscEBGHACcDazJzS2ZuBdYAc0vbfpm5NjMTuLq2LUmakKaOdwKSpI6XwD9HRAL/MzOXAV2ZubG0PwZ0lfnpwKO1ddeX2GDx9W3iO4iIxVRnxOnq6qKnp2fEb2L79u07tV6TzKEzclhyVB9d+zDpj4M5jF4OFtiSpKG8LTM3RMRrgTUR8f16Y2ZmKb5HTSnqlwHMnj07u7u7R7yNnp4edma9JplDZ+Rw9tIbWHJUH2dO8uNgDqOXg5eISJIGlZkbys9NwFeorqF+vFzeQfm5qSy+ATi0tvqMEhssPqNNXJImLAtsSdKAImLfiHh1/zxwEnAfsAroHwlkIXB9mV8FLCijicwBtpVLSW4CToqIaeXmxpOAm0rbUxExp4wesqC2LUmakHapwHboJkna7XUB346I7wG3ATdk5leBi4F3RMRDwNvLa4DVwMNAL/BZ4ByAzNwCXAjcXqZPlBhlmX8o6/wAuHEM3pckjZomrsH+1cx8ova6f+imiyNiaXn9EXYcuul4qmGZjq8N3TSb6kaaOyNiVbnLvH/oplupOu252PFK0pjJzIeBN7WJbwZObBNP4NwBtrUcWN4mfgfwxl1OVpI6xGhcIuLQTZIkSZq0drXA7h+66c4yhBKM8dBNkiRJUifZ1UtExn3oJtj18VF3x/EXzWHnLDmqD8DxUc2h43KQJE0cu1Rg14duiogdhm7KzI0jGLqpuyXewwiGbtrV8VF3x/EXzWHnnL30BgDHRzWHjstBkjRx7PQlIg7dJEmSJL3crpzB7gK+UkbOmwr878z8akTcDlwbEYuAHwFnluVXA6dSDcP0LPBuqIZuioj+oZvg5UM3XQXsQzV6iCOISJIkqaPtdIHt0E2SJEnSy/kkR0mSJKlBFtiSJElSgyywJUmSpAZZYEuSJEkNssCWJEmSGmSBLUmSJDXIAluSJElqkAW2JEmS1CALbEmSJKlBFtiSJElSgyywJUmSpAZZYEuSJEkNssCWJEmSGmSBLUmSJDXIAluSJElqkAW2JEmS1CALbEn/f3v3G7NVXcdx/P0Zd6D2RyCmMWFxs2kbW5uRGq1/Bq6QnPTANVotWWtubKlYW4N41LPUVtrm5pzWsixTJGVuLiOtZ9yGJPgHUYwKEEIfYLU2HfPbg98XuabIrXDO7xyu6/Part3nnAv4fe7vde7v/eM651zHzMzMGuQJtpmZmZlZg3o/wZa0VNJOSbskrek6j5mZNc+93syGSa8n2JKmALcAlwILgK9KWtBtKjMza5J7vZkNm15PsIGLgF0R8beIeA24G1jecSYzM2uWe72ZDZWxrgNM4hxgz8D6XuATb/5Dkq4CrsrV/0ra+S7HmQW8fEIJm+MM/cnANTDrmq93nqMPtXCGE8/w4TaCDKlJe30DfR5O3X3JGVrgPu8MDWU4Zq/v+wT7HYmI24DbTvTvS9oSERc0GMkZTuEMfcnhDM5gR51sn4d+vI7O4AzOMBoZ+n6KyD5g7sD6nNxmZmbDw73ezIZK3yfYfwHOlTQuaSqwAtjYcSYzM2uWe72ZDZVenyISEYclfRv4PTAF+FlEPN3CUCd12LEhzlD0IQP0I4czFM4w5Nzrq3OGwhkKZygazaCIaPLfMzMzMzMbaX0/RcTMzMzM7JTiCbaZmZmZWYNGeoIt6XxJmyU9IWmLpItyuyT9NG/Zu13SwpZzXC3pWUlPS7phYPvazLBT0hfbzJDjfVdSSJqV69XqIOnGrMF2Sb+TNH3guWp16OJ2zZLmSnpU0jO5D1yb22dK+oOk5/PrjApZpkj6q6QHc31c0kTW47d5AVqb40+XtD73hR2SPlm7DpKuy9fhKUm/kXRa7TpYc9zn35Kjsz6f43Xe67vo8zluL3p9130+xxz+Xh8RI/sAHgYuzeVlwJ8Glh8CBCwCJlrM8HlgEzAt18/KrwuAbcA0YBx4AZjSYo65lAuM/gHM6qAOXwDGcvl64PradaBcXPUCMB+YmuMuqLAfzgYW5vL7gefy+74BWJPb1xypSctZvgP8Gngw1+8BVuTyrcCqlsf/BfCtXJ4KTK9ZB8oNT3YDpw98/ytr18GPRl9T9/mjOTrt8zlep72+qz6fY/ei13fd53Ocoe/1I/0ONhDAB3L5TODFXF4O3BnFZmC6pNktZVgF/DAiXgWIiIMDGe6OiFcjYjewi3I74bb8BPgepSZHVKtDRDwcEYdzdTPlc3CPZKhVh05u1xwR+yNiay7/B9hB+eFfTmlC5Ncvt5lD0hzgS8DtuS5gMbC+RgZJZwKfBe4AiIjXIuIQletA+XSl0yWNAWcA+6lYB2uc+/xRnfZ56EWv76TPQz96fdd9PscciV4/6hPs1cCNkvYAPwLW5vZj3bb3nJYynAd8Jg9J/FnShbUzSFoO7IuIbW96qmYdBn2T8o5K7Qxdfb9vkDQP+BgwAZwdEfvzqQPA2S0PfxPll+/ruf5B4NDAL8O26zEOvAT8PA9f3i7pvVSsQ0Tso/SCf1Ka7SvA49StgzVrNe7zfezz0E2v77zPQ6e9/ia67fMwIr2+15+D3QRJm4APHeOpdcAS4LqIuE/SVyj/m7qkcoYxYCbl0NyFwD2S5lfO8H3KYbtWHS9DRDyQf2YdcBi4q+08fSPpfcB9wOqI+Hd5Y6GIiJDU2mdqSroMOBgRj0u6uK1xJjEGLASujogJSTdTDhO+oUIdZlDeRRkHDgH3AkvbGs+a4T7/jjJU6fOT5XCv767X96TPw4j0+qGfYEfE2zZSSXcC1+bqveQhExq+be8kGVYBG6Kc8POYpNeBWbUySPooZQfblj/kc4CtKhcCVatDZlkJXAYsyXrQdIZJdHa7ZknvoTTcuyJiQ27+l6TZEbE/D9kefPt/4aR9Crhc0jLgNMoh9Zsph4vH8n/0bddjL7A3IiZyfT2l6daswyXA7oh4CUDSBkptatbB3iX3+eNnqNnnj5djIM9Kuuv1nfV56LzX96HPw4j0+lE/ReRF4HO5vBh4Ppc3At9QsQh4ZeCwRdPup1wAg6TzKCf7v5wZVkiaJmkcOBd4rOnBI+LJiDgrIuZFxDzKjr8wIg5QsQ6SllIOW10eEf8beKpKHVInt2vOc+DuAHZExI8HntoIXJnLVwIPtJUhItZGxJzcB1YAj0TE14BHgSsqZTgA7JH0kdy0BHiGinWgHC5cJOmMfF2OZKhWB2uc+3xP+jz0otd30ueh+17fhz6fOUaj10eFK2f7+gA+TTnnZhvlPKiP53YBt1CuNH4SuKDFDFOBXwFPAVuBxQPPrcsMO8mr4CvU5O8cvbq8Zh12Uc6LeyIft3ZRB8oV9c/leOsq7ocBbB/4/pdRzo37I2VCsAmYWSnPxRy9unw+5ZfcLsq7f9NaHvt8YEvW4n5gRu06AD8Ans2fyV9SPtWgah38aPT1dJ9/a55O+nyO13mv76LPD+yLvej1Xfb5HHPoe71vlW5mZmZm1qBRP0XEzMzMzKxRnmCbmZmZmTXIE2wzMzMzswZ5gm1mZmZm1iBPsM3MzMzMGuQJtpmZmZlZgzzBNjMzMzNr0P8BtfEJ7XU3OkgAAAAASUVORK5CYII=\n", + "text/plain": [ + "
" + ] + }, + "metadata": { + "needs_background": "light" + }, + "output_type": "display_data" + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "{'3999356047_R01C01_unmeth': 9.232241569522861e-05,\n", + " '3999356047_R02C01_unmeth': 0.0001472295440055741,\n", + " '3999356047_R03C01_unmeth': 0.00011443153360447612}\n" + ] + } + ], + "source": [ + "pre = ['3999356047_R01C01','3999356047_R02C01','3999356047_R03C01']\n", + "post = ['GSM2815705_3999356047_R01C01','GSM2815706_3999356047_R02C01','GSM2815707_3999356047_R03C01']\n", + "samples = []\n", + "mean_diff = {}\n", + "for idx,sample in enumerate(pre):\n", + " vmeth = m_v111_samples[sample]['noob_meth']\n", + " vunmeth = m_v111_samples[sample]['noob_unmeth']\n", + " delta = vmeth - _meth[post[idx]]\n", + " delta.name = sample + '_meth'\n", + " samples.append( delta )\n", + " delta = vunmeth - _unmeth[post[idx]]\n", + " delta.name = sample + '_unmeth'\n", + " samples.append( delta )\n", + " mean_diff[delta.name] = delta.mean()\n", + "diff = pd.concat(samples, axis=1)\n", + "diff.hist(bins=200, figsize=(12,12), range=[-80,80])\n", + "plt.show()\n", + "pp(mean_diff)" + ] + }, + { + "cell_type": "markdown", + "id": "c87803e3", + "metadata": {}, + "source": [ + "# methylprep v1.4.6 vs minfi (noob meth, noob unmeth)\n", + "difference is slightly off because methylprep v1.4.6 adds poobah before NOOB step for cleaner data." + ] + }, + { + "cell_type": "code", + "execution_count": 4, + "id": "0f2150e7", + "metadata": { + "scrolled": false + }, + "outputs": [ + { + "data": { + "image/png": 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\n", + "text/plain": [ + "
" + ] + }, + "metadata": { + "needs_background": "light" + }, + "output_type": "display_data" + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "{'3999356047_R01C01_unmeth': -0.21698295997381803,\n", + " '3999356047_R02C01_unmeth': -0.0015504255523069302,\n", + " '3999356047_R03C01_unmeth': -0.5322082452443613}\n" + ] + } + ], + "source": [ + "pre = ['3999356047_R01C01','3999356047_R02C01','3999356047_R03C01']\n", + "post = ['GSM2815705_3999356047_R01C01','GSM2815706_3999356047_R02C01','GSM2815707_3999356047_R03C01']\n", + "samples = []\n", + "mean_diff = {}\n", + "for idx,sample in enumerate(pre):\n", + " vmeth = m_v146_samples[sample]['noob_meth']\n", + " vunmeth = m_v146_samples[sample]['noob_unmeth']\n", + " delta = vmeth - _meth[post[idx]]\n", + " delta.name = sample + '_meth'\n", + " samples.append( delta )\n", + " delta = vunmeth - _unmeth[post[idx]]\n", + " delta.name = sample + '_unmeth'\n", + " samples.append( delta )\n", + " mean_diff[delta.name] = delta.mean()\n", + "diff = pd.concat(samples, axis=1)\n", + "diff.hist(bins=200, figsize=(12,12), range=[-80,80])\n", + "plt.show()\n", + "pp(mean_diff)" + ] + }, + { + "cell_type": "markdown", + "id": "79ad3621", + "metadata": {}, + "source": [ + "# methylprep v1.5.2 --minfi vs minfi (noob meth, noob unmeth)\n", + "\n", + "ran: `methylprep process -d . --minfi`\n", + "\n", + " (base) /Volumes/LEGX/GEO/test_pipeline/GSE105018_minfi$ python -m methylprep process -d . --minfi\n", + " dict_items([('data_dir', PosixPath('.')), ('array_type', None), ('manifest', None), \n", + " ('sample_sheet', None), ('no_sample_sheet', False), ('sample_name', None), ('betas', False),\n", + " ('m_value', False), ('batch_size', None), ('uncorrected', False), ('no_export', True),\n", + " ('no_meta_export', True), ('bit', 'float32'), ('save_control', False), ('poobah', False),\n", + " ('export_poobah', False), ('minfi', True), ('no_quality_mask', False), ('all', False)])\n", + "\n", + " (base) /Volumes/LEGX/GEO/test_pipeline/GSE105018_minfi$ python -m methylprep process -d . --minfi --all\n", + " dict_items([('data_dir', PosixPath('.')), ('array_type', None), ('manifest', None), ('sample_sheet', None),\n", + " ('no_sample_sheet', False), ('sample_name', None), ('betas', True), ('m_value', True), ('batch_size', None),\n", + " ('uncorrected', True), ('no_export', True), ('no_meta_export', True), ('bit', 'float32'),\n", + " ('save_control', True), ('poobah', True), ('export_poobah', True), ('minfi', True),\n", + " ('no_quality_mask', False), ('all', True)])\n", + "\n", + "differences here: \n", + "- quality mask and poobah are turned on with --all --minfi\n", + "- --minfi is NOT affected by poobah, since poobah should not run with these params.\n", + "- confirmed, it runs linear dye with NOOB under these conditions\n" + ] + }, + { + "cell_type": "code", + "execution_count": 5, + "id": "9d055a37", + "metadata": { + "scrolled": false + }, + "outputs": [ + { + "data": { + "image/png": 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\n", + "text/plain": [ + "
" + ] + }, + "metadata": { + "needs_background": "light" + }, + "output_type": "display_data" + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "{'3999356047_R01C01_2unmeth': -16.023457486296667,\n", + " '3999356047_R01C01_unmeth': 2.487625001627748,\n", + " '3999356047_R02C01_2unmeth': -9.717331450138039,\n", + " '3999356047_R02C01_unmeth': 4.113145371007935,\n", + " '3999356047_R03C01_2unmeth': -14.802453797568242,\n", + " '3999356047_R03C01_unmeth': 5.715650101636692}\n" + ] + } + ], + "source": [ + "# CLI methylprep with --minfi params\n", + "mmeth_minfi = pd.read_pickle(Path(minpath,'noob_meth_values.pkl')).sort_index()\n", + "munmeth_minfi = pd.read_pickle(Path(minpath,'noob_unmeth_values.pkl')).sort_index()\n", + "\n", + "pre = ['3999356047_R01C01','3999356047_R02C01','3999356047_R03C01']\n", + "post = ['GSM2815705_3999356047_R01C01','GSM2815706_3999356047_R02C01','GSM2815707_3999356047_R03C01']\n", + "samples = []\n", + "mean_diff = {}\n", + "for idx,sample in enumerate(pre):\n", + " delta = m_minfi_meth[pre[idx]] - _meth[post[idx]]\n", + " delta.name = sample + '_meth'\n", + " samples.append( delta )\n", + " delta = m_minfi_unmeth[pre[idx]] - _unmeth[post[idx]]\n", + " delta.name = sample + '_unmeth'\n", + " samples.append( delta )\n", + " mean_diff[delta.name] = delta.mean()\n", + "\n", + " delta = mmeth_minfi[pre[idx]] - _meth[post[idx]]\n", + " delta.name = sample + '_2meth'\n", + " samples.append( delta )\n", + " delta = munmeth_minfi[pre[idx]] - _unmeth[post[idx]]\n", + " delta.name = sample + '_2unmeth'\n", + " samples.append( delta )\n", + " mean_diff[delta.name] = delta.mean()\n", + "\n", + "diff = pd.concat(samples, axis=1)\n", + "diff.hist(bins=200, figsize=(16,12)) #, range=[-80,80])\n", + "plt.show()\n", + "pp(mean_diff)" + ] + }, + { + "cell_type": "markdown", + "id": "d42a9dbf", + "metadata": {}, + "source": [ + "# how sesame noob was calculated\n", + "\n", + " library(BiocManager)\n", + " library(sesame)\n", + " library(preprocessCore)\n", + " # after upgrading R3.6-->R4.1 I had to move cache: https://bioconductor.org/packages/devel/bioc/vignettes/ExperimentHub/inst/doc/ExperimentHub.html#default-caching-location-update\n", + " # sesameDataCache(\"MM285\") # run this once, before you can read mouse\n", + " # version 3.13 DOCS HERE: https://bioconductor.org/packages/devel/bioc/vignettes/sesame/inst/doc/sesame.html\n", + "\n", + " in_dir = paste0('/Volumes/LEGX/GEO/test_pipeline/GSE105018_sesame')\n", + "\n", + " savefiles = function(sdfs, idx, thisname) {\n", + " x_thisframe <- data.frame(rbind( IG(sdfs[[idx]]), IR(sdfs[[idx]]), II(sdfs[[idx]]) ))\n", + " x_filename <- paste0('sesame_', thisname ,'.csv', sep=\"\")\n", + " x_file <- file.path(in_dir, x_filename)\n", + " write.csv(x_file, x=x_thisframe, row.names=TRUE, na=\"\")\n", + " }\n", + "\n", + " # RAW INTENSITY\n", + " sdfs <- lapply(searchIDATprefixes(in_dir), readIDATpair) #readIdat is for one file; readIDATpair is for pairs.\n", + " savefiles(sdfs, 1, 'raw')\n", + "\n", + " # INFER\n", + " sdfs = lapply(sdfs, inferTypeIChannel, verbose=TRUE)\n", + " savefiles(sdfs, 1, 'infer')\n", + "\n", + " # POOBAH ssets <- lapply(ssets, detectionMask) # poobah and qualityMask now set in sdf@extra$mask by readIDATpair\n", + " sdfs <- lapply(sdfs, pOOBAH)\n", + " savefiles(sdfs, 1, 'poobah') # doesn't save the poobah filtered part\n", + "\n", + " ### -- these don't return a modified sdf. so they need a new location.\n", + " # NOOB\n", + " sdfs.noob <- lapply(sdfs, noob) # creates a bunch more sdf objects in a list here\n", + " savefiles(sdfs.noob, 1, 'noob') # noob doesn't actually exclude poobah probes here? unless auto\n", + "\n", + " # DYE-BIAS\n", + " sdfs.dye <- lapply(sdfs.noob, dyeBiasCorrTypeINorm)\n", + " savefiles(sdfs.dye, 1, 'dye')\n", + "\n", + " # BETAS\n", + " betas <- lapply(sdfs.dye, getBetas) # this uses @extra$mask\n", + " write.csv(file.path(in_dir, 'sesame_mouse_betas.csv'), x=betas, row.names=TRUE, na=\"\")\n", + "\n", + " Rprof(tf <- \"log.log\", memory.profiling = TRUE)\n", + " idat_dir = paste0('/Volumes/LEGX/Barnes/mouse_test/')\n", + " betas= openSesame(idat_dir, BPPARAM=BiocParallel::MulticoreParam(2))\n", + " Rprof (NULL) ; print(summaryRprof(tf))\n", + " system.time( openSesame(idat_dir, BPPARAM=BiocParallel::MulticoreParam(2)) )\n", + " # sesame_version user system elapsed\n", + " # 3.13 42.691 2.705 23.291\n", + "\n" + ] + }, + { + "cell_type": "markdown", + "id": "1405218c", + "metadata": {}, + "source": [ + "# --- sesame raw is exactly the same, except for infer-switched values ---" + ] + }, + { + "cell_type": "code", + "execution_count": 6, + "id": "d78c4e57", + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "M 0\n", + "U 0\n", + "dtype: int64\n" + ] + }, + { + "data": { + "image/png": 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\n", + "text/plain": [ + "
" + ] + }, + "metadata": { + "needs_background": "light" + }, + "output_type": "display_data" + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "{'3999356047_R01C01_infer': M 0.0\n", + "U 0.0\n", + "dtype: float64,\n", + " '3999356047_R01C01_rawmeth': 7.969323106328988,\n", + " '3999356047_R01C01_rawunmeth': 0.2741497635485838}\n" + ] + } + ], + "source": [ + "# --------- sesame raw is exactly the same, except for NaN values -------\n", + "t2_path = Path('/Volumes/LEGX/GEO/test_pipeline/GSE105018_test') # methylprep --all sesame v1.5.1\n", + "sample = ['3999356047_R01C01']\n", + "mean_diff = {}\n", + "\n", + "# pulling from the pickles added NaNs and chart wouldn't plot normally. So using CSV output instead.\n", + "#raw_meth = pd.read_pickle(Path(t2_path,'meth_values.pkl')).sort_index()\n", + "#raw_unmeth = pd.read_pickle(Path(t2_path,'unmeth_values.pkl')).sort_index()\n", + "\n", + "raw_samples = {}\n", + "for _csv in Path(t2_path, '3999356047').rglob('*.csv'):\n", + " sample_name = '_'.join(Path(_csv).stem.split('_')[:2])\n", + " _sample = pd.read_csv(_csv).set_index('IlmnID')\n", + " raw_samples[sample_name] = _sample\n", + "raw_meth = raw_samples[sample[0]]['meth']\n", + "raw_unmeth = raw_samples[sample[0]]['unmeth']\n", + " \n", + "sespath = Path('/Volumes/LEGX/GEO/test_pipeline/GSE105018_sesame')\n", + "ses_raw = pd.read_csv(Path(sespath, 'sesame_raw.csv')).set_index('Unnamed: 0')\n", + "ses_infer = pd.read_csv(Path(sespath, 'sesame_infer.csv')).set_index('Unnamed: 0')\n", + "#-- ses_raw is identical to ses_infer because I need to apply the sdfs@extra$IGG / $IRR TRUE mask to swap probes\n", + "# manually, and besides, openSesame no longer runs this step.\n", + "ses_raw = ses_raw[ ~ses_raw.index.str.startswith('rs') ].sort_index()\n", + "ses_infer = ses_infer[ ~ses_infer.index.str.startswith('rs') ].sort_index()\n", + "\n", + "delta_infer = ses_raw - ses_infer\n", + "delta_infer.name = sample[0] + '_infer'\n", + "mean_diff[delta_infer.name] = delta_infer.mean()\n", + "print( (ses_raw - ses_infer).sum() )\n", + "\n", + "delta_rawmeth = raw_meth - ses_raw['M'].sort_index()\n", + "delta_rawmeth.name = sample[0] + '_rawmeth'\n", + "mean_diff[delta_rawmeth.name] = delta_rawmeth.mean()\n", + "\n", + "delta_rawunmeth = raw_unmeth - ses_raw['U'].sort_index()\n", + "delta_rawunmeth.name = sample[0] + '_rawunmeth'\n", + "mean_diff[delta_rawunmeth.name] = delta_rawunmeth.mean()\n", + "\n", + "diff = pd.concat([delta_rawmeth.dropna(), delta_rawunmeth.dropna()], axis=1) # delta_meth, delta_unmeth, \n", + "diff.hist(bins=100, figsize=(12,4), range=[-10,10])\n", + "\n", + "plt.show()\n", + "pp(mean_diff)\n" + ] + }, + { + "cell_type": "markdown", + "id": "f52fc761", + "metadata": {}, + "source": [ + "# sesame noob, before dye step vs mprep" + ] + }, + { + "cell_type": "code", + "execution_count": 7, + "id": "f9c6f07a", + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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\n", + "text/plain": [ + "
" + ] + }, + "metadata": { + "needs_background": "light" + }, + "output_type": "display_data" + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "{'3999356047_R01C01_meth': 9.168439357283818,\n", + " '3999356047_R01C01_unmeth': 9.347128209487355}\n" + ] + } + ], + "source": [ + "t3_path = Path('/Volumes/LEGX/GEO/test_pipeline/GSE105018_test_nodye') \n", + "# df= m.make_pipeline(steps=['infer_channel_switch', 'poobah', 'quality_mask', 'noob'], sesame=True, debug=True)\n", + "t3_meth = pd.read_pickle(Path(t3_path,'noob_meth_values.pkl')).sort_index() # methylprep_noob_meth --sesame\n", + "t3_unmeth = pd.read_pickle(Path(t3_path,'noob_unmeth_values.pkl')).sort_index() # methylprep_noob_unmeth --sesame\n", + "sespath = Path('/Volumes/LEGX/GEO/test_pipeline/GSE105018_sesame')\n", + "ses_noob = pd.read_csv(Path(sespath, 'sesame_noob.csv')).set_index('Unnamed: 0')\n", + "\n", + "mean_diff = dict()\n", + "sample = ['3999356047_R01C01'] #['3999356047_R01C01'] # ,'3999356047_R02C01','3999356047_R03C01']\n", + "\n", + "delta_meth = t3_meth[sample[0]] - ses_noob['M']\n", + "delta_meth.name = sample[0] + '_meth'\n", + "mean_diff[delta_meth.name] = delta_meth.mean()\n", + "\n", + "delta_unmeth = t3_unmeth[sample[0]] - ses_noob['U']\n", + "delta_unmeth.name = sample[0] + '_unmeth'\n", + "mean_diff[delta_unmeth.name] = delta_unmeth.mean()\n", + "\n", + "diff = pd.concat([delta_meth, delta_unmeth], axis=1) \n", + "diff.hist(bins=200, figsize=(12,4), range=[-20,20])\n", + "\n", + "plt.show()\n", + "pp(mean_diff)" + ] + }, + { + "cell_type": "markdown", + "id": "73925665", + "metadata": {}, + "source": [ + "# v.1.5.2 (build_pipeline, sesame, noob only) vs minfi" + ] + }, + { + "cell_type": "code", + "execution_count": 8, + "id": "880ce510", + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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\n", + "text/plain": [ + "
" + ] + }, + "metadata": { + "needs_background": "light" + }, + "output_type": "display_data" + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "{'3999356047_R01C01_meth': 752.8287423527173,\n", + " '3999356047_R01C01_unmeth': -715.0082786625668,\n", + " '3999356047_R02C01_meth': 780.0390570411862,\n", + " '3999356047_R02C01_unmeth': -699.2420467418908,\n", + " '3999356047_R03C01_meth': 778.6796715287064,\n", + " '3999356047_R03C01_unmeth': -670.208918424742}\n" + ] + } + ], + "source": [ + "_meth = pd.read_csv(Path(minpath,'minfi_noob_meth.csv')).set_index('Unnamed: 0').sort_index()\n", + "_meth.index.name='IlmnID'\n", + "_unmeth = pd.read_csv(Path(minpath,'minfi_noob_unmeth.csv')).set_index('Unnamed: 0').sort_index()\n", + "_unmeth.index.name='IlmnID'\n", + "\n", + "pre = ['3999356047_R01C01','3999356047_R02C01','3999356047_R03C01']\n", + "post = ['GSM2815705_3999356047_R01C01','GSM2815706_3999356047_R02C01','GSM2815707_3999356047_R03C01']\n", + "samples = []\n", + "mean_diff = {}\n", + "for idx,sample in enumerate(pre):\n", + " delta = t1_meth[pre[idx]] - _meth[post[idx]]\n", + " delta.name = sample + '_meth'\n", + " samples.append( delta )\n", + " mean_diff[delta.name] = delta.mean()\n", + " delta = t1_unmeth[pre[idx]] - _unmeth[post[idx]]\n", + " delta.name = sample + '_unmeth'\n", + " samples.append( delta )\n", + " mean_diff[delta.name] = delta.mean()\n", + "diff = pd.concat(samples, axis=1)\n", + "diff.hist(bins=200, figsize=(12,12), range=[-2000,2000])\n", + "plt.show()\n", + "pp(mean_diff)\n" + ] + }, + { + "cell_type": "markdown", + "id": "fbde5207", + "metadata": {}, + "source": [ + "# sesame vs methylprep --all (default)" + ] + }, + { + "cell_type": "code", + "execution_count": 9, + "id": "731bf6a7", + "metadata": { + "scrolled": false + }, + "outputs": [ + { + "data": { + "image/png": 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\n", + "text/plain": [ + "
" + ] + }, + "metadata": { + "needs_background": "light" + }, + "output_type": "display_data" + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "{'3999356047_R01C01_beta': -0.0021256439592542867,\n", + " '3999356047_R01C01_noob+dye_meth': -19.087137169690244,\n", + " '3999356047_R01C01_noob+dye_unmeth': 25.25839165295372}\n" + ] + } + ], + "source": [ + "sample = ['3999356047_R01C01'] #['3999356047_R01C01'] # ,'3999356047_R02C01','3999356047_R03C01']\n", + "\n", + "t2_path = Path('/Volumes/LEGX/GEO/test_pipeline/GSE105018_test') # methylprep --all sesame v1.5.1\n", + "# running make_pipeline(steps=[all], sesame=True, debug=True) shows that linear dye is happening\n", + "# and sesame=None in middle\n", + "t2_meth = pd.read_pickle(Path(t2_path,'noob_meth_values.pkl')).sort_index() # methylprep_noob_meth --sesame\n", + "t2_unmeth = pd.read_pickle(Path(t2_path,'noob_unmeth_values.pkl')).sort_index() # methylprep_noob_unmeth --sesame\n", + "t2_beta = pd.read_pickle(Path(t2_path, 'beta_values.pkl')).sort_index()\n", + "\n", + "sespath = Path('/Volumes/LEGX/GEO/test_pipeline/GSE105018_sesame')\n", + "ses_dye = pd.read_csv(Path(sespath, 'sesame_dye.csv')).set_index('Unnamed: 0')\n", + "ses_beta= pd.read_csv(Path(sespath, 'sesame_betas.csv')).set_index('Unnamed: 0')['GSM2815705_3999356047_R01C01']\n", + "\n", + "mean_diff = dict()\n", + "sample = ['3999356047_R01C01'] #['3999356047_R01C01'] # ,'3999356047_R02C01','3999356047_R03C01']\n", + "ses_beta.name = sample[0]\n", + "\n", + "delta_dyemeth = t2_meth[sample[0]] - ses_dye['M']\n", + "delta_dyemeth.name = sample[0] + '_noob+dye_meth'\n", + "mean_diff[delta_dyemeth.name] = delta_dyemeth.mean()\n", + "\n", + "delta_dyeunmeth = t2_unmeth[sample[0]] - ses_dye['U']\n", + "delta_dyeunmeth.name = sample[0] + '_noob+dye_unmeth'\n", + "mean_diff[delta_dyeunmeth.name] = delta_dyeunmeth.mean()\n", + "\n", + "ses_beta.name = sample[0]\n", + "delta_beta = t2_beta[sample[0]] - ses_beta\n", + "delta_beta.name = sample[0] + '_beta'\n", + "mean_diff[delta_beta.name] = delta_beta.mean()\n", + "\n", + "diff = pd.concat([delta_dyemeth, delta_dyeunmeth, delta_beta], axis=1) \n", + "diff.hist(bins=200, figsize=(12,12), range=[-100,100])\n", + "\n", + "plt.show()\n", + "pp(mean_diff)" + ] + }, + { + "cell_type": "markdown", + "id": "43aa28f8", + "metadata": {}, + "source": [ + "\n", + " THESE ARE harder to match up than the output, but luckily the output matches exactly.\n", + " the mprep oob doesn't retain the probe names in index.\n", + " minfiG = pd.read_csv(Path(minpath, 'minfi_oobG.csv')).set_index('Unnamed: 0')[['GSM2815705_3999356047_R01C01']].sort_index()\n", + " minfiR = pd.read_csv(Path(minpath, 'minfi_oobR.csv')).set_index('Unnamed: 0')[['GSM2815705_3999356047_R01C01']].sort_index()\n", + " oobG = pd.read_csv(Path(t1_path, 't1_oobG.csv')).set_index('Unnamed: 0').sort_index() #[['3999356047_R01C01']]\n", + " oobR = pd.read_csv(Path(t1_path, 't1_oobR.csv')).set_index('Unnamed: 0').sort_index() #[['3999356047_R01C01']]\n", + " NOTE: methylprep oobG and oobR contain SNP probes too, but minfi's do not.\n", + " NOTE: 450k ctl_man ctrl_green has 850 rows, but ctrl_red only has 848 rows. Why?\n", + " SDG attrs: ibG has 396374, ibR has 439279, but debug msg says: \"ibG 442672 ibR 528482 oobG 178406 oobR 92596\"\n", + " ... because debug msg combines + stacks meth + unmeth channels ... but parts don't add up.\n", + " \n", + " \n", + "df = m.make_pipeline('.', steps=['noob'], sesame=False, debug=True) result\n", + "\n", + " {'3999356047_R01C01_meth': 0.1463333209121593,\n", + " '3999356047_R01C01_unmeth': -0.34745759293849976,\n", + " '3999356047_R02C01_meth': 0.06334636886553159,\n", + " '3999356047_R02C01_unmeth': -0.03053014181060626,\n", + " '3999356047_R03C01_meth': -0.29185560562537033,\n", + " '3999356047_R03C01_unmeth': -0.6494391272821136}\n", + " \n", + "df = m.make_pipeline('.', steps=['noob', 'poobah'], sesame=False, debug=True) result\n", + "\n", + " {'3999356047_R01C01_meth': -14.268274723784089,\n", + " '3999356047_R01C01_unmeth': -19.03968117029445,\n", + " '3999356047_R02C01_meth': -11.299798091666727,\n", + " '3999356047_R02C01_unmeth': -14.77085999493948,\n", + " '3999356047_R03C01_meth': -16.611835004047588,\n", + " '3999356047_R03C01_unmeth': -22.2066433930435}\n", + " NOTE: this matches CLI --minfi --poobah --no_quality_mask exactly!\n", + "\n", + "python -m methylprep -v process -d . --minfi --poobah --no_quality_mask\n", + "\n", + " {'3999356047_R01C01_meth': -14.268274723784089,\n", + " '3999356047_R01C01_unmeth': -19.03968117029445,\n", + " '3999356047_R02C01_meth': -11.299798091666727,\n", + " '3999356047_R02C01_unmeth': -14.77085999493948,\n", + " '3999356047_R03C01_meth': -16.611835004047588,\n", + " '3999356047_R03C01_unmeth': -22.2066433930435}\n", + " \n", + "python -m methylprep -v process -d . --all --minfi\n", + "\n", + " {'3999356047_R01C01_meth': 752.8287423527173,\n", + " '3999356047_R01C01_unmeth': -715.0082786625668,\n", + " '3999356047_R02C01_meth': 780.0390570411862,\n", + " '3999356047_R02C01_unmeth': -699.2420467418908,\n", + " '3999356047_R03C01_meth': 778.6796715287064,\n", + " '3999356047_R03C01_unmeth': -670.208918424742}\n", + " (this looks like --all turns on dye-bias despite the --minfi flag)\n", + " \n", + " FIXED this on 2021-07-13. NEW:\n", + " {'3999356047_R01C01_meth': -16.023457486296667,\n", + " '3999356047_R01C01_unmeth': -0.11924747245559411,\n", + " '3999356047_R02C01_meth': -9.717331450138039,\n", + " '3999356047_R02C01_unmeth': 4.388272385892256,\n", + " '3999356047_R03C01_meth': -14.802453797568242,\n", + " '3999356047_R03C01_unmeth': 1.6000359669370239} \n", + "\n", + "python -m methylprep -v process -d . --minfi\n", + "\n", + " {'3999356047_R01C01_meth': 12.10730234391046,\n", + " '3999356047_R01C01_unmeth': 2.487625001627748,\n", + " '3999356047_R02C01_meth': 14.130577026997036,\n", + " '3999356047_R02C01_unmeth': 4.113145371007935,\n", + " '3999356047_R03C01_meth': 15.179276701251773,\n", + " '3999356047_R03C01_unmeth': 5.715650101636692}\n", + " (looks like infer-channel and poobah filtering turns on, but noob/dye is linear)\n", + "\n", + "\n" + ] + }, + { + "cell_type": "markdown", + "id": "b9c4e89a", + "metadata": {}, + "source": [ + "# mprep --minfi --all output vs minfi (meth, unmeth) " + ] + }, + { + "cell_type": "code", + "execution_count": 11, + "id": "e8f9fed6", + "metadata": { + "scrolled": false + }, + "outputs": [ + { + "data": { + "image/png": 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\n", + "text/plain": [ + "
" + ] + }, + "metadata": { + "needs_background": "light" + }, + "output_type": "display_data" + }, + { + "name": "stdout", + "output_type": "stream", + "text": [ + "{'3999356047_R01C01_meth': -16.023457486296667,\n", + " '3999356047_R01C01_unmeth': -0.11924747245559411,\n", + " '3999356047_R02C01_meth': -9.717331450138039,\n", + " '3999356047_R02C01_unmeth': 4.388272385892256,\n", + " '3999356047_R03C01_meth': -14.802453797568242,\n", + " '3999356047_R03C01_unmeth': 1.6000359669370239}\n" + ] + } + ], + "source": [ + "pre = ['3999356047_R01C01','3999356047_R02C01','3999356047_R03C01']\n", + "post = ['GSM2815705_3999356047_R01C01','GSM2815706_3999356047_R02C01','GSM2815707_3999356047_R03C01']\n", + "samples = []\n", + "mean_diff = {}\n", + "for idx,sample in enumerate(pre):\n", + " delta = mmeth_minfi[pre[idx]] - _meth[post[idx]]\n", + " delta.name = sample + '_unmeth'\n", + " samples.append( delta )\n", + " mean_diff[delta.name] = delta.mean()\n", + " delta = munmeth_minfi[pre[idx]] - _unmeth[post[idx]]\n", + " delta.name = sample + '_meth'\n", + " samples.append( delta )\n", + " mean_diff[delta.name] = delta.mean()\n", + " \n", + "diff = pd.concat(samples, axis=1)\n", + "diff.hist(bins=200, figsize=(12,12), range=[-2000,2000])\n", + "plt.show()\n", + "pp(mean_diff)" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "id": "fe416674", + "metadata": {}, + "outputs": [], + "source": [] + } + ], + "metadata": { + "kernelspec": { + "display_name": "Python 3", + "language": "python", + "name": "python3" + }, + "language_info": { + "codemirror_mode": { + "name": "ipython", + "version": 3 + }, + "file_extension": ".py", + "mimetype": "text/x-python", + "name": "python", + "nbconvert_exporter": "python", + "pygments_lexer": "ipython3", + "version": "3.8.8" + } + }, + "nbformat": 4, + "nbformat_minor": 5 +} diff --git a/docs/debug_notebooks/sesame_epic_one_GSE147430.R b/docs/debug_notebooks/sesame_epic_one_GSE147430.R index 39a6284..7558f96 100644 --- a/docs/debug_notebooks/sesame_epic_one_GSE147430.R +++ b/docs/debug_notebooks/sesame_epic_one_GSE147430.R @@ -18,7 +18,7 @@ sdfs <- lapply(searchIDATprefixes(in_dir), readIDATpair) #readIdat is for one fi savefiles(sdfs, 1, 'raw') # INFER -sdfs = lapply(sdfs, inferTypeIChannel, verbose=TRUE) +sdfs.infer = lapply(sdfs, inferTypeIChannel, verbose=TRUE) savefiles(sdfs, 1, 'infer') # POOBAH ssets <- lapply(ssets, detectionMask) # poobah and qualityMask now set in sdf@extra$mask by readIDATpair diff --git a/docs/example_data/read_geo/GSE111165_test.csv b/docs/example_data/read_geo/GSE111165_test.csv new file mode 100644 index 0000000..bf2d948 --- /dev/null +++ b/docs/example_data/read_geo/GSE111165_test.csv @@ -0,0 +1,201 @@ +,Unnamed: 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diff --git a/docs/example_data/read_geo/GSE133355_processed_test.xlsx b/docs/example_data/read_geo/GSE133355_processed_test.xlsx new file mode 100644 index 0000000..a1196ef Binary files /dev/null and b/docs/example_data/read_geo/GSE133355_processed_test.xlsx differ diff --git a/docs/example_data/read_geo/GSE72120_test.txt b/docs/example_data/read_geo/GSE72120_test.txt new file mode 100644 index 0000000..545bfa3 --- /dev/null +++ b/docs/example_data/read_geo/GSE72120_test.txt @@ -0,0 +1,201 @@ + ID_REF 2963 Unmethylated signal 2963 Methylated signal 2963 Detection Pval 2962 Unmethylated signal 2962 Methylated signal 2962 Detection Pval 2691 Unmethylated signal 2691 Methylated signal 2691 Detection Pval 2908 Unmethylated signal 2908 Methylated signal 2908 Detection Pval 2926 Unmethylated signal 2926 Methylated signal 2926 Detection Pval 2891 Unmethylated signal 2891 Methylated signal 2891 Detection Pval 2982 Unmethylated signal 2982 Methylated signal 2982 Detection Pval 4220 Unmethylated signal 4220 Methylated signal 4220 Detection Pval 4229 Unmethylated signal 4229 Methylated signal 4229 Detection Pval 4249 Unmethylated signal 4249 Methylated signal 4249 Detection Pval 4239 Unmethylated signal 4239 Methylated signal 4239 Detection Pval 4227 Unmethylated signal 4227 Methylated signal 4227 Detection Pval 6090 Unmethylated signal 6090 Methylated signal 6090 Detection Pval 2939 Unmethylated signal 2939 Methylated signal 2939 Detection Pval 2966 Unmethylated signal 2966 Methylated signal 2966 Detection Pval 7009 Unmethylated signal 7009 Methylated signal 7009 Detection Pval 2531 Unmethylated signal 2531 Methylated signal 2531 Detection Pval 6108 Unmethylated signal 6108 Methylated signal 6108 Detection Pval 2685 Unmethylated signal 2685 Methylated signal 2685 Detection Pval 6076 Unmethylated signal 6076 Methylated signal 6076 Detection Pval 7008 Unmethylated signal 7008 Methylated signal 7008 Detection Pval 2767 Unmethylated signal 2767 Methylated signal 2767 Detection Pval 6131 Unmethylated signal 6131 Methylated signal 6131 Detection Pval 6130 Unmethylated signal 6130 Methylated signal 6130 Detection Pval 2717 Unmethylated signal 2717 Methylated signal 2717 Detection Pval 7007 Unmethylated signal 7007 Methylated signal 7007 Detection Pval 2449 Unmethylated signal 2449 Methylated signal 2449 Detection Pval 2981 Unmethylated signal 2981 Methylated signal 2981 Detection Pval 2910 Unmethylated signal 2910 Methylated signal 2910 Detection Pval 6069 Unmethylated signal 6069 Methylated signal 6069 Detection Pval 2885 Unmethylated signal 2885 Methylated signal 2885 Detection Pval 2776 Unmethylated signal 2776 Methylated signal 2776 Detection Pval 2913 Unmethylated signal 2913 Methylated signal 2913 Detection Pval 2956 Unmethylated signal 2956 Methylated signal 2956 Detection Pval 6133 Unmethylated signal 6133 Methylated signal 6133 Detection Pval 2761 Unmethylated signal 2761 Methylated signal 2761 Detection Pval 2740 Unmethylated signal 2740 Methylated signal 2740 Detection Pval 6017 Unmethylated signal 6017 Methylated signal 6017 Detection Pval 2618 Unmethylated signal 2618 Methylated signal 2618 Detection Pval 4269 Unmethylated signal 4269 Methylated signal 4269 Detection Pval 2466 Unmethylated signal 2466 Methylated signal 2466 Detection Pval 2818 Unmethylated signal 2818 Methylated signal 2818 Detection Pval 6073 Unmethylated signal 6073 Methylated signal 6073 Detection Pval 2783 Unmethylated signal 2783 Methylated signal 2783 Detection Pval 4248 Unmethylated signal 4248 Methylated signal 4248 Detection Pval 2454 Unmethylated signal 2454 Methylated signal 2454 Detection Pval 2778 Unmethylated signal 2778 Methylated signal 2778 Detection Pval 6126 Unmethylated signal 6126 Methylated signal 6126 Detection Pval 6013 Unmethylated signal 6013 Methylated signal 6013 Detection Pval 7004 Unmethylated signal 7004 Methylated signal 7004 Detection Pval 2763 Unmethylated signal 2763 Methylated signal 2763 Detection Pval 2307 Unmethylated signal 2307 Methylated signal 2307 Detection Pval 2680 Unmethylated signal 2680 Methylated signal 2680 Detection Pval 2666 Unmethylated signal 2666 Methylated signal 2666 Detection Pval 7005 Unmethylated signal 7005 Methylated signal 7005 Detection Pval 2406 Unmethylated signal 2406 Methylated signal 2406 Detection Pval 2488 Unmethylated signal 2488 Methylated signal 2488 Detection Pval 7006 Unmethylated signal 7006 Methylated signal 7006 Detection Pval 6036 Unmethylated signal 6036 Methylated signal 6036 Detection Pval 4270 Unmethylated signal 4270 Methylated signal 4270 Detection Pval 2866 Unmethylated signal 2866 Methylated signal 2866 Detection Pval 2736 Unmethylated signal 2736 Methylated signal 2736 Detection Pval 2914 Unmethylated signal 2914 Methylated signal 2914 Detection Pval 6134 Unmethylated signal 6134 Methylated signal 6134 Detection Pval 4285 Unmethylated signal 4285 Methylated signal 4285 Detection Pval 2865 Unmethylated signal 2865 Methylated signal 2865 Detection Pval 2368 Unmethylated signal 2368 Methylated signal 2368 Detection Pval 2667 Unmethylated signal 2667 Methylated signal 2667 Detection Pval 6127 Unmethylated signal 6127 Methylated signal 6127 Detection Pval 7002 Unmethylated signal 7002 Methylated signal 7002 Detection Pval 2396 Unmethylated signal 2396 Methylated signal 2396 Detection Pval 2743 Unmethylated signal 2743 Methylated signal 2743 Detection Pval +0 cg13869341 928 5432 0.0 942 5657 0.0 559 4762 0.0 768 5777 0.0 1328 10919 0.0 2373 11907 0.0 635 2963 0.0 323 2827 0.0 462 3121 0.0 666 3133 0.0 1307 7090 0.0 2353 10327 0.0 3270 17808 0.0 1966 17845 0.0 3435 19044 0.0 3717 18295 0.0 3702 20468 0.0 3897 19361 0.0 1878 16552 0.0 1138 16219 0.0 1775 18375 0.0 1670 18446 0.0 2962 18718 0.0 2939 19885 0.0 2248 14771 0.0 3650 15052 0.0 2315 15983 0.0 2494 16011 0.0 2714 17402 0.0 2438 17060 0.0 2754 14953 0.0 1569 16792 0.0 2002 15544 0.0 1887 17115 0.0 2754 17903 0.0 2585 18680 0.0 2627 16900 0.0 1674 18414 0.0 3696 18118 0.0 1663 17504 0.0 1634 16742 0.0 2391 16026 0.0 1947 15653 0.0 2981 16247 0.0 1594 17544 0.0 1922 17967 0.0 2475 17932 0.0 2088 16959 0.0 2291 15104 0.0 2087 16918 0.0 2508 17894 0.0 3317 18134 0.0 1635 18516 0.0 2671 19660 0.0 2767 14819 0.0 1555 16881 0.0 2586 16211 0.0 1515 16087 0.0 1633 17273 0.0 1496 17690 0.0 3929 16470 0.0 3691 16556 0.0 2502 17762 0.0 1655 17522 0.0 1555 17555 0.0 2523 19205 0.0 1775 16362 0.0 2485 17685 0.0 1467 15858 0.0 2481 16096 0.0 2673 17818 0.0 1623 17271 0.0 +1 cg14008030 1946 5823 0.0 3001 4738 0.0 1371 5700 0.0 1896 5801 0.0 3714 10746 0.0 4057 11408 0.0 1086 2045 0.0 860 2602 0.0 944 2542 0.0 1062 3490 0.0 3534 9048 0.0 5166 12076 0.0 4699 8730 0.0 4137 10496 0.0 5981 10087 0.0 5434 11635 0.0 4360 11224 0.0 5609 11084 0.0 5141 8657 0.0 4870 9510 0.0 5210 10545 0.0 4668 11876 0.0 7167 11520 0.0 5595 12699 0.0 3870 9095 0.0 5334 9507 0.0 3484 10376 0.0 5365 9955 0.0 6088 10394 0.0 6366 11160 0.0 4105 8187 0.0 5620 9161 0.0 5126 10086 0.0 6147 9902 0.0 4921 10682 0.0 5360 11513 0.0 5091 8467 0.0 3778 9085 0.0 6186 8851 0.0 4346 10806 0.0 4467 10615 0.0 3375 11746 0.0 4472 7997 0.0 5743 8805 0.0 5552 9741 0.0 4956 10350 0.0 4134 11493 0.0 4656 12804 0.0 4430 7788 0.0 5620 9101 0.0 5124 10414 0.0 6107 10022 0.0 6229 10846 0.0 5113 11373 0.0 5326 7377 0.0 5335 8343 0.0 3467 10639 0.0 3979 11934 0.0 4710 11067 0.0 4253 12900 0.0 4973 7825 0.0 5516 8348 0.0 4767 9290 0.0 6106 10156 0.0 4653 11156 0.0 5018 10742 0.0 6097 7874 0.0 5780 9173 0.0 4861 9636 0.0 4015 10835 0.0 5426 9399 0.0 5788 10429 0.0 +2 cg12045430 3724 246 0.0 3539 204 0.0 3163 261 0.0 4004 218 0.0 6792 309 0.0 7298 438 0.0 1662 204 0.0 1821 194 0.0 1887 151 0.0 2254 170 0.0 4506 257 0.0 7036 407 0.0 10580 803 0.0 11515 786 0.0 11177 938 0.0 11790 916 0.0 11744 861 0.0 11774 1101 0.0 9643 959 0.0 9728 747 0.0 11340 935 0.0 11506 817 0.0 12173 1043 0.0 11606 1258 0.0 10229 678 0.0 9701 622 0.0 9608 750 0.0 9995 581 0.0 11050 744 0.0 10310 742 0.0 8762 423 0.0 10280 865 0.0 9777 583 0.0 10063 615 0.0 11229 673 0.0 11276 882 0.0 8456 825 0.0 9405 909 0.0 10279 909 0.0 9610 860 0.0 9165 851 0.0 8151 896 0.0 7752 715 0.0 9201 793 0.0 8985 808 0.0 10055 715 0.0 9275 696 0.0 8589 786 0.0 8239 602 0.0 9796 657 0.0 9963 684 0.0 10435 698 0.0 9220 703 0.0 9581 882 0.0 8165 607 0.0 9821 590 0.0 9756 514 0.0 9552 704 0.0 9678 637 0.0 10108 814 0.0 9134 866 0.0 10018 814 0.0 10126 925 0.0 9311 678 0.0 8934 806 0.0 10050 799 0.0 8837 653 0.0 9387 965 0.0 8718 773 0.0 9269 811 0.0 9085 805 0.0 8731 836 0.0 +3 cg20826792 3664 356 0.0 3290 270 0.0 3244 438 0.0 3428 351 0.0 6669 651 0.0 6960 1019 0.0 1651 214 0.0 1467 286 0.0 1778 241 0.0 2140 271 0.0 4239 415 0.0 5751 590 0.0 9722 1929 0.0 9196 2166 0.0 10019 1606 0.0 10324 1979 0.0 10161 1795 0.0 10302 2169 0.0 9319 1816 0.0 8531 1749 0.0 9437 2072 0.0 9790 2005 0.0 10515 2055 0.0 10774 2118 0.0 8449 1448 0.0 8555 1616 0.0 8849 1238 0.0 8868 1295 0.0 9347 1217 0.0 9924 1153 0.0 8149 1285 0.0 9303 1633 0.0 8938 1261 0.0 9664 1549 0.0 9409 1325 0.0 10090 1693 0.0 8939 1826 0.0 9711 1859 0.0 9835 1712 0.0 9251 1699 0.0 8375 1489 0.0 8763 1654 0.0 8535 1266 0.0 9656 1562 0.0 9206 1339 0.0 9839 1563 0.0 9861 1676 0.0 8966 1306 0.0 7940 1161 0.0 9227 1456 0.0 9198 1581 0.0 9479 1488 0.0 9155 1782 0.0 9936 1459 0.0 7504 1070 0.0 8993 1422 0.0 8628 1538 0.0 8926 1110 0.0 8917 1251 0.0 9239 1437 0.0 9177 1875 0.0 8933 1401 0.0 9969 1563 0.0 9462 1427 0.0 9290 1539 0.0 10592 1261 0.0 8627 1462 0.0 8450 1685 0.0 8667 1526 0.0 8381 1436 0.0 8722 1348 0.0 9401 1457 0.0 +4 cg00381604 2314 206 0.0 2164 217 0.0 2386 220 0.0 2446 226 0.0 4605 330 0.0 4939 312 0.0 1268 163 0.0 1127 116 0.0 1062 159 0.0 1467 149 0.0 3125 244 0.0 5139 410 0.0 8534 686 0.0 8216 735 0.0 8911 745 0.0 8596 756 0.0 9284 843 0.0 8608 902 0.0 7517 668 0.0 7504 582 0.0 8399 689 0.0 8556 778 0.0 8872 747 0.0 9200 920 0.0 7778 492 0.0 6977 559 0.0 6754 628 0.0 7705 453 0.0 8300 508 0.0 8571 632 0.0 6552 286 0.0 7810 481 0.0 8028 487 0.0 8000 581 0.0 8444 585 0.0 8804 733 0.0 7875 784 0.0 9126 868 0.0 7967 740 0.0 7083 715 0.0 8223 551 0.0 7315 679 0.0 7522 553 0.0 8005 700 0.0 7433 743 0.0 7903 553 0.0 8497 646 0.0 7720 761 0.0 6868 527 0.0 8170 530 0.0 7575 505 0.0 7698 836 0.0 7345 569 0.0 8307 585 0.0 6652 469 0.0 7625 551 0.0 7804 587 0.0 7394 540 0.0 7641 594 0.0 7344 578 0.0 7677 689 0.0 7904 616 0.0 7843 560 0.0 7706 623 0.0 7540 497 0.0 8720 766 0.0 7572 649 0.0 7304 496 0.0 6940 630 0.0 7245 692 0.0 7882 602 0.0 7589 718 0.0 +5 cg20253340 282 835 0.0 295 690 0.0 743 1368 0.0 641 1245 0.0 1114 3043 0.0 1270 4633 0.0 560 910 0.0 516 1016 0.0 293 608 0.0 574 1486 0.0 661 1696 0.0 1301 3147 0.0 3403 3344 0.0 1451 2730 0.0 1789 1886 0.0 5693 5458 0.0 3582 5292 0.0 4565 5282 0.0 4184 3337 0.0 3069 4308 0.0 3188 6612 0.0 4798 5490 0.0 5003 6992 0.0 5100 6109 0.0 2166 5242 0.0 2702 5088 0.0 2812 4951 0.0 4422 5043 0.0 2530 5583 0.0 2191 3311 0.0 2769 3697 0.0 4454 3230 0.0 4762 5275 0.0 3313 4994 0.0 5677 6335 0.0 4587 6559 0.0 3845 4294 0.0 6062 4756 0.0 4122 5301 0.0 2461 4855 0.0 3906 5269 0.0 3871 6514 0.0 1915 3204 0.0 3841 5793 0.0 2580 5814 0.0 2594 5488 0.0 1642 7330 0.0 2263 7078 0.0 4057 3898 0.0 3491 5435 0.0 2212 3439 0.0 2747 2804 0.0 2732 5412 0.0 4239 6497 0.0 3610 3693 0.0 2778 2544 0.0 2508 4714 0.0 2978 5386 0.0 3365 6669 0.0 3760 5466 0.0 2516 4734 0.0 4112 3568 0.0 4564 4561 0.0 3466 6331 0.0 2647 5135 0.0 5003 6305 0.0 2733 2913 0.0 3455 4626 0.0 1759 3379 0.0 3413 5679 0.0 1859 2538 0.0 2729 5168 0.0 +6 cg21870274 303 1077 0.0 232 1733 0.0 508 2137 0.0 474 2203 0.0 984 4508 0.0 1037 5650 0.0 405 1270 0.0 326 1225 0.0 239 1002 0.0 355 2066 0.0 656 2691 0.0 971 5422 0.0 1712 4425 0.0 922 3139 0.0 742 2720 0.0 3183 6742 0.0 2391 5588 0.0 2490 6312 0.0 1726 4274 0.0 1334 5205 0.0 2530 6976 0.0 2469 6449 0.0 2563 7554 0.0 2411 7021 0.0 1523 4679 0.0 1758 5180 0.0 1736 4501 0.0 2117 5828 0.0 2217 5146 0.0 1199 4908 0.0 1782 3826 0.0 1938 4936 0.0 2200 5616 0.0 1885 5813 0.0 2490 6773 0.0 2159 6861 0.0 2308 4504 0.0 2981 5141 0.0 1870 5007 0.0 1547 4643 0.0 1813 5376 0.0 1387 5403 0.0 1515 3503 0.0 1913 5069 0.0 1775 4746 0.0 1742 5699 0.0 2176 6050 0.0 1983 6487 0.0 2024 4611 0.0 2089 5791 0.0 1408 4157 0.0 1789 4985 0.0 1489 5247 0.0 2435 6734 0.0 1756 4086 0.0 1265 3515 0.0 2121 5460 0.0 1383 5472 0.0 1711 6490 0.0 1893 6043 0.0 1799 4491 0.0 1704 4654 0.0 2093 5240 0.0 1994 5226 0.0 1180 4382 0.0 2401 6409 0.0 1437 3552 0.0 1771 4801 0.0 1227 3647 0.0 2210 5397 0.0 1090 3219 0.0 1440 4985 0.0 +7 cg03130891 414 119 0.0 367 140 0.0 292 125 0.0 309 155 0.0 412 235 0.0 813 358 0.0 192 109 0.0 244 117 0.0 231 118 0.0 154 145 0.166666666666667 322 269 0.0 443 309 0.0033333333333333 504 281 0.0083333333333333 689 281 0.0 504 240 0.0133333333333333 679 303 0.0 897 310 0.0 730 295 0.0 458 269 0.00166666666666671 674 297 0.0 2161 336 0.0 1452 370 0.0 776 328 0.0 723 407 0.005 887 304 0.0 692 300 0.0 1679 279 0.0 1035 234 0.0 764 248 0.0 1099 356 0.0 562 189 0.0 614 344 0.0 670 252 0.0 887 257 0.0 1766 306 0.0 745 213 0.0 837 374 0.0 936 478 0.0 822 310 0.0 633 501 0.0733333333333334 777 407 0.005 829 639 0.0 935 302 0.0 934 339 0.0 697 322 0.01 637 430 0.251666666666667 753 398 0.00166666666666671 588 520 0.21666666666666698 586 194 0.0 624 246 0.0 690 305 0.0 695 244 0.0 603 328 0.0 1048 383 0.0 386 207 0.0 507 253 0.0033333333333333 507 278 0.00166666666666671 497 393 0.00166666666666671 1239 315 0.0 599 356 0.0 521 269 0.0033333333333333 634 312 0.0 582 308 0.0083333333333333 566 334 0.00166666666666671 906 295 0.0 723 410 0.0 869 241 0.0 1077 309 0.0 554 294 0.015 635 332 0.0 932 397 0.0 721 469 0.0 +8 cg24335620 762 3701 0.0 586 3703 0.0 648 4209 0.0 705 4480 0.0 1287 7680 0.0 1579 11447 0.0 483 2129 0.0 522 2039 0.0 414 2118 0.0 650 2885 0.0 1288 6863 0.0 1522 11216 0.0 4284 8476 0.0 1505 9086 0.0 2492 8818 0.0 2529 10278 0.0 2532 10381 0.0 3447 10493 0.0 3239 7618 0.0 3989 8542 0.0 3789 9839 0.0 2741 11063 0.0 3674 11764 0.0 4222 12218 0.0 3636 7896 0.0 2605 8762 0.0 2472 8542 0.0 2376 9164 0.0 2189 9532 0.0 2937 9767 0.0 2233 6938 0.0 3176 8070 0.0 1925 8430 0.0 2017 8733 0.0 2446 9715 0.0 3063 10489 0.0 2858 8293 0.0 3858 8353 0.0 2380 9552 0.0 3110 9952 0.0 2327 9214 0.0 2800 10891 0.0 2695 7754 0.0 2575 8983 0.0 2165 8643 0.0 1942 10531 0.0 1731 9752 0.0 3455 11411 0.0 2159 7581 0.0 2418 8536 0.0 2891 9090 0.0 3048 9790 0.0 2451 9982 0.0 2497 10168 0.0 2703 6875 0.0 2648 8168 0.0 3408 9198 0.0 2434 9410 0.0 2389 9579 0.0 3963 11280 0.0 2749 7653 0.0 2385 8171 0.0 2309 8890 0.0 2984 9828 0.0 3084 8944 0.0 2648 10414 0.0 2002 7656 0.0 2329 8519 0.0 2842 8999 0.0 2668 8711 0.0 3245 8670 0.0 3058 10487 0.0 +9 cg16162899 181 527 0.0 222 229 0.0 219 1117 0.0 241 807 0.0 287 1276 0.0 546 4122 0.0 130 603 0.0 150 528 0.0 165 732 0.0 160 942 0.0 276 1941 0.0 373 3291 0.0 551 1993 0.0 664 3534 0.0 515 2825 0.0 403 3557 0.0 665 3353 0.0 794 5676 0.0 374 3015 0.0 598 3565 0.0 626 3265 0.0 630 3493 0.0 703 2670 0.0 622 2803 0.0 389 1736 0.0 390 2104 0.0 620 3960 0.0 316 899 0.0 477 3556 0.0 515 2411 0.0 476 3142 0.0 550 3900 0.0 413 3193 0.0 656 4666 0.0 459 4359 0.0 712 3395 0.0 763 2601 0.0 623 1730 0.0 654 2545 0.0 523 1144 0.0 677 1849 0.0 541 2840 0.0 469 1898 0.0 630 1843 0.0 715 3352 0.0 731 3941 0.0 540 2187 0.0 870 4207 0.0 482 2574 0.0 564 2572 0.0 470 1837 0.0 461 2420 0.0 615 3180 0.0 545 3944 0.0 394 1940 0.0 663 2736 0.0 541 3392 0.0 491 1710 0.0 513 3646 0.0 515 3527 0.0 510 2038 0.0 750 3236 0.0 631 2613 0.0 366 2305 0.0 430 1456 0.0 667 2597 0.0 465 1644 0.0 530 3236 0.0 618 2529 0.0 641 3804 0.0 548 1596 0.0 592 2101 0.0 +10 cg17149495 257 599 0.0 169 544 0.0 227 615 0.0 284 578 0.0 398 973 0.0 495 1667 0.0 163 347 0.0 277 356 0.0 189 394 0.0 157 356 0.0 270 1031 0.0 367 1233 0.0 867 3066 0.0 707 2381 0.0 856 2077 0.0 776 3597 0.0 681 3777 0.0 918 3771 0.0 734 2647 0.0 757 3168 0.0 643 2457 0.0 817 2509 0.0 765 4551 0.0 914 4609 0.0 464 1528 0.0 772 2432 0.0 605 2008 0.0 592 2988 0.0 727 2599 0.0 767 1157 0.0 462 3036 0.0 518 1998 0.0 592 2994 0.0 826 1955 0.0 560 3264 0.0 687 3645 0.0 852 3185 0.0 1057 4268 0.0 730 2670 0.0 868 1557 0.0 662 3337 0.0 721 2922 0.0 616 2469 0.0 795 1553 0.0 664 3423 0.0 741 2468 0.0 750 2811 0.0 714 1630 0.0 556 2466 0.0 632 2056 0.0 590 3039 0.0 731 3190 0.0 561 3434 0.0 534 3362 0.0 421 2673 0.0 655 3221 0.0 696 2002 0.0 701 2337 0.0 608 2371 0.0 487 3597 0.0 814 2543 0.0 961 2643 0.0 652 3081 0.0 702 2227 0.0 590 1881 0.0 832 2329 0.0 655 1827 0.0 608 2550 0.0 841 1913 0.0 773 2965 0.0 713 2240 0.0 672 3294 0.0 +11 cg22802167 417 7750 0.0 344 7119 0.0 616 8381 0.0 449 9201 0.0 716 14912 0.0 1490 17783 0.0 201 3768 0.0 283 3692 0.0 333 3577 0.0 397 5167 0.0 1126 11842 0.0 1065 18065 0.0 763 12850 0.0 1262 14245 0.0 1387 15576 0.0 896 18119 0.0 839 16230 0.0 1272 17592 0.0 886 12696 0.0 1168 14246 0.0 1237 18180 0.0 1180 17362 0.0 1019 18586 0.0 878 18355 0.0 712 12506 0.0 817 14451 0.0 921 14498 0.0 622 14135 0.0 994 16250 0.0 777 17141 0.0 614 11546 0.0 835 14430 0.0 906 16140 0.0 1163 14557 0.0 698 15272 0.0 788 17241 0.0 939 13442 0.0 706 12961 0.0 777 15020 0.0 718 16303 0.0 946 15493 0.0 1053 17016 0.0 801 12404 0.0 983 14844 0.0 829 15218 0.0 1169 17006 0.0 999 16295 0.0 1395 19020 0.0 516 11961 0.0 836 13294 0.0 774 15103 0.0 834 14961 0.0 973 15896 0.0 959 17147 0.0 551 10765 0.0 748 12579 0.0 752 14681 0.0 773 15708 0.0 740 15572 0.0 664 19281 0.0 708 11043 0.0 771 12878 0.0 873 15821 0.0 730 15317 0.0 787 15653 0.0 795 15840 0.0 630 12393 0.0 812 13669 0.0 1120 15778 0.0 792 15192 0.0 1073 15774 0.0 735 16687 0.0 +12 cg24669183 437 1687 0.0 270 1656 0.0 670 2595 0.0 414 2242 0.0 833 4537 0.0 1226 6591 0.0 229 814 0.0 249 1144 0.0 242 853 0.0 379 1261 0.0 668 3897 0.0 702 4702 0.0 1233 4281 0.0 1948 6415 0.0 1132 3945 0.0 1530 6352 0.0 1598 6169 0.0 1795 5773 0.0 827 4245 0.0 1491 5263 0.0 818 2997 0.0 1785 7054 0.0 2338 7610 0.0 2290 6751 0.0 1521 4699 0.0 1927 5748 0.0 1756 5749 0.0 1697 5214 0.0 1339 6197 0.0 1219 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586 2250 0.0 411 2253 0.0 481 1953 0.0 665 2691 0.0 477 2139 0.0 495 1873 0.0 344 1732 0.0 522 2583 0.0 617 2750 0.0 640 2210 0.0 +199 cg24664590 1664 185 0.0 1246 129 0.0 2129 195 0.0 2297 283 0.0 4308 503 0.0 5412 533 0.0 1072 120 0.0 1205 220 0.0 1107 137 0.0 1235 253 0.0 3672 493 0.0 3982 626 0.0 8343 328 0.0 8660 507 0.0 10428 570 0.0 8128 566 0.0 7006 387 0.0 8578 437 0.0 6445 376 0.0 7898 393 0.0 8159 481 0.0 7657 442 0.0 8386 570 0.0 8524 547 0.0 6695 363 0.0 7067 380 0.0 7068 399 0.0 8323 346 0.0 7502 307 0.0 9231 380 0.0 6533 301 0.0 7192 306 0.0 7484 448 0.0 7548 351 0.0 7802 366 0.0 8616 459 0.0 6858 489 0.0 8218 493 0.0 8010 509 0.0 6047 656 0.0 7272 568 0.0 6904 785 0.0 6110 372 0.0 7848 551 0.0 7337 523 0.0 8278 588 0.0 7426 559 0.0 6816 781 0.0 6786 319 0.0 7348 509 0.0 7681 423 0.0 7933 457 0.0 7745 545 0.0 6392 483 0.0 6356 312 0.0 8168 469 0.0 7165 494 0.0 6884 463 0.0 7261 486 0.0 7574 474 0.0 7101 286 0.0 7118 392 0.0 7847 447 0.0 7495 386 0.0 5635 428 0.0 7964 580 0.0 7806 419 0.0 6848 400 0.0 5622 388 0.0 7102 507 0.0 8617 561 0.0 7349 579 0.0 diff --git a/docs/release-history.md b/docs/release-history.md index 392e06b..d4ad149 100644 --- a/docs/release-history.md +++ b/docs/release-history.md @@ -1,26 +1,35 @@ # Release History -## v1.5.0 +## v1.5.2 +- Bug fix: added 'Extended_Type' into control_probes.pkl output. Required by methylcheck.plot_controls(). +- Minor bug fixes and improved unit test coverage. +- Fixed bug where `process --minfi` was not working with `--all`. Added more test coverage for CLI. +- updated read_geo to handle more edge cases +- deprecated some never-used functions. + - instead of methylprep.files.idat.RunInfo use IdatDataset( verbose=True ) + + +## v1.5.0, v1.5.1 - MAJOR refactor/overhaul of all the internal classes. This was necessary to fully support the mouse array. - - new SigSet class object that mirror's sesame's SigSet and SigDF object. - - Combines idats, manifest, and sample sheet into one object that is inherited by SampleDataContainer - - RawDataset, MethylationDataset, ProbeSubtype all deprecated and replaced by SigSet - - SampleDataContainer class is now basically the SigSet plus all pipeline processing settings - - new mouse manifest covers all probes and matches sesame's output - - Processing will work even if a batch of IDATs have differing probe counts for same array_type, though those - differing probes in question may not be saved. - - unit tests confirm that methylprep, sesame, and minfi beta values output match to within 1% of each other now. Note that the intermediate stages of processing (after NOOB and after DYE) do not match - with sesame in this version. Can be +/- 100 intensity units, likely due to differences in order of - steps and/or oob/mask probes used. +- new SigSet class object that mirror's sesame's SigSet and SigDF object. +- Combines idats, manifest, and sample sheet into one object that is inherited by SampleDataContainer +- RawDataset, MethylationDataset, ProbeSubtype all deprecated and replaced by SigSet +- SampleDataContainer class is now basically the SigSet plus all pipeline processing settings +- new mouse manifest covers all probes and matches sesame's output +- Processing will work even if a batch of IDATs have differing probe counts for same array_type, though those +differing probes in question may not be saved. +- unit tests confirm that methylprep, sesame, and minfi beta values output match to within 1% of each other now. Note that the intermediate stages of processing (after NOOB and after DYE) do not match +with sesame in this version. Can be +/- 100 intensity units, likely due to differences in order of +steps and/or oob/mask probes used. ## v1.4.7 - mouse manifest updated to conform with illumina Genome Studio / sesame probe naming convention. - - mouse_probes.pkl now includes different probe types. Previously, if a probe type was 'mu' (multi) - or 'rp' (repeat) or IlmnID started with 'uk' (unknown?), it was moved to experimental mouse_probes.pkl. - This was about 6300 probes. - Now, all 'Multi' and 'Random' probes are moved and stored in mouse_probes.pkl, about 30,000. - - mouse manifest has a 'design' column with tons of human-readable notes on different probe origins, - including analogous EPIC human-mapped probes. +- mouse_probes.pkl now includes different probe types. Previously, if a probe type was 'mu' (multi) +or 'rp' (repeat) or IlmnID started with 'uk' (unknown?), it was moved to experimental mouse_probes.pkl. +This was about 6300 probes. +Now, all 'Multi' and 'Random' probes are moved and stored in mouse_probes.pkl, about 30,000. +- mouse manifest has a 'design' column with tons of human-readable notes on different probe origins, +including analogous EPIC human-mapped probes. ## v1.4.6 - pipeline CSV output will now include meth, unmeth, beta, and m-values for all probes, including failed probes. @@ -80,4 +89,4 @@ - methylprep.download has a new all-encompassing pipeline that will read GEO data sets and convert any data file type into a pickle of beta_values, whether from idats or processed matrix files. -## Older versions exist on pypi, but no changelog +## Older versions exist on pypi, but no changelog diff --git a/methylprep/cli.py b/methylprep/cli.py index 1429b72..f8c6c14 100644 --- a/methylprep/cli.py +++ b/methylprep/cli.py @@ -253,9 +253,10 @@ def cli_process(cmd_args): args.no_meta_export = True args.poobah = True args.export_poobah = True - args.minfi = False args.no_quality_mask = False + #print(vars(args).items()) + run_pipeline( args.data_dir, array_type=args.array_type, diff --git a/methylprep/files/idat.py b/methylprep/files/idat.py index f35f137..07b80e7 100644 --- a/methylprep/files/idat.py +++ b/methylprep/files/idat.py @@ -87,13 +87,12 @@ class IdatSectionCode(IntEnum): # Object Definitions # ---------------------------------------------------------------------------- - +""" DEPRECATED: use IdatDataset(... verbose=True) instead. class RunInfo(): - """A dataclass defining IDAT run information. + '''A dataclass defining IDAT run information. Arguments: - idat_file {file-like} -- An open IDAT file. - """ + idat_file {file-like} -- An open IDAT file.''' __slots__ = [ 'run_time', @@ -104,12 +103,13 @@ class RunInfo(): ] def __init__(self, idat_file): - """Initializes the RunInfo data class by reading the provided idat_file.""" + # Initializes the RunInfo data class by reading the provided idat_file. self.run_time = read_string(idat_file) self.block_type = read_string(idat_file) self.block_pars = read_string(idat_file) self.block_code = read_string(idat_file) self.code_version = read_string(idat_file) +""" class IdatDataset(): """Validates and parses an Illumina IDAT file. diff --git a/methylprep/files/manifests.py b/methylprep/files/manifests.py index 769881c..b6e3326 100644 --- a/methylprep/files/manifests.py +++ b/methylprep/files/manifests.py @@ -302,9 +302,8 @@ def get_data_types(self): return data_types def get_probe_details(self, probe_type, channel=None): - """given a probe type (I, II, SnpI, SnpII, Control) and a channel (Channel.RED | Channel.GREEN), - This will return info needed to map probes to their names (e.g. cg0031313 or rs00542420), - which are NOT in the idat files.""" + """used by infer_channel_switch. Given a probe type (I, II, SnpI, SnpII, Control) and a channel (Channel.RED | Channel.GREEN), + this will return info needed to map probes to their names (e.g. cg0031313 or rs00542420), which are NOT in the idat files.""" if not isinstance(probe_type, ProbeType): raise Exception('probe_type is not a valid ProbeType') diff --git a/methylprep/models/sigset.py b/methylprep/models/sigset.py index 9ca3be3..428cb12 100644 --- a/methylprep/models/sigset.py +++ b/methylprep/models/sigset.py @@ -117,9 +117,9 @@ class SigSet(): [x] IG - Type-I Grn channel probes; [x] oobG - Out-of-band Grn channel probes (matching Type-I Red channel probes in number); [x] oobR - Out-of-band Red channel probes (matching Type-I Grn channel probes in number); - [-] ctl - control probes. + [x] ctrl_green, ctrl_red - control probes. [x] methylated, unmethylated, snp_methylated, snp_unmethylated - [x] fg_green, fg_red (opposite of oobG and oobR) + [x] fg_green, fg_red (opposite of oobG and oobR) AKA ibG, ibR for in-band probes. - just tidying up how we access this stuff, and trying to stick to IlmnID everywhere because the illumina_id within IDAT files is no longer unique as a ref. - I checked again, and no other array breaks these rules. But sounds like Bret won’t stick to this pattern going forward with designs. So I suspect other software will break with new arrays, unless they rewrite for this too. @@ -305,6 +305,8 @@ def update_probe_means(self, noob_green, noob_red, red_factor=None): upstream: container.sigset.update_probe_means(noob_green, noob_red) replaces 'bg_corrected' column with 'noob_Meth' or 'noob_Unmeth' column. + + does NOT update ctrl_red or ctrl_green; these are updated within the NOOB function because structually different. """ for probe_subset, decoder_parts in self.subsets.items(): @@ -387,14 +389,15 @@ def update_probe_means(self, noob_green, noob_red, red_factor=None): self.__minfi_noob = False self.__linear_dye = True if red_factor is not None else False + """ # from raw_dataset; may no longer be needed, but kept for testing against new approach 2021 def get_oob_controls(self, green_idat, red_idat, manifest, include_rs=True): - """ Out-of-bound controls are the mean intensity values for the + ''' Out-of-bound controls are the mean intensity values for the channel in the opposite channel's probes (IG oob and IR oob) .. todo:: TEST -- does this give same output as SigSet.oobG and oobR? - """ + ''' param_sets = [ {'channel': Channel.RED, 'idat': green_idat, 'manifest': manifest}, {'channel': Channel.GREEN, 'idat': red_idat, 'manifest': manifest}, @@ -451,6 +454,7 @@ def get_oob_controls(self, green_idat, red_idat, manifest, include_rs=True): ).rename(columns={'mean_value': 'Unmeth'}) oobR.drop(['AddressA_ID', 'AddressB_ID'], axis=1) return (oobG.sort_index(), oobR.sort_index()) + """ # from raw_dataset def filter_oob_probes(self, channel, manifest, idat_dataset, include_rs=True): diff --git a/methylprep/models/sketchy_probes.py b/methylprep/models/sketchy_probes.py index ce25d6a..e25f672 100644 --- a/methylprep/models/sketchy_probes.py +++ b/methylprep/models/sketchy_probes.py @@ -4,8 +4,9 @@ from importlib import resources # py3.7+ pkg_namespace = 'methylprep.models' +""" def sketchy_probes_warning(filepath): - """ not implemented anywhere yet """ + ''' not implemented anywhere yet ''' # used to warn user if running a Catalog_ID that contains sketchy illumina probes with resources.path(pkg_namespace, 'illumina_sketchy_probes_996.npy') as probe_filepath: @@ -22,6 +23,7 @@ def sketchy_probes_warning(filepath): return Path(filepath).name else: return None +""" with resources.path(pkg_namespace, 'qualityMask450.txt.gz') as probe_filepath: qualityMask450 = pd.read_csv(probe_filepath)['x'] diff --git a/methylprep/processing/p_value_probe_detection.py b/methylprep/processing/p_value_probe_detection.py index ffa78f9..449cc88 100644 --- a/methylprep/processing/p_value_probe_detection.py +++ b/methylprep/processing/p_value_probe_detection.py @@ -9,8 +9,80 @@ import numpy as np +def _pval_sesame_preprocess(data_container): + """Performs p-value detection of low signal/noise probes. This ONE SAMPLE version uses meth/unmeth before it is contructed into a _SampleDataContainer__data_frame. + - returns a dataframe of probes and their detected p-value levels. + - this will be saved to the csv output, so it can be used to drop probes at later step. + - output: index are probes (IlmnID or illumina_id); one column [poobah_pval] contains the sample p-values. + - called by pipeline CLI --poobah option. + - confirmed that this version produces identical results to the pre-v1.5.0 version on 2021-06-16 + """ + # 2021-03-22 assumed 'mean_value' for red and green MEANT meth and unmeth (OOBS), respectively. + funcG = ECDF(data_container.oobG['Unmeth'].values) + funcR = ECDF(data_container.oobR['Meth'].values) + pIR = pd.DataFrame( + index=data_container.IR.index, + data=1-np.maximum(funcR(data_container.IR['Meth']), funcR(data_container.IR['Unmeth'])), + columns=['poobah_pval']) + pIG = pd.DataFrame( + index=data_container.IG.index, + data=1-np.maximum(funcG(data_container.IG['Meth']), funcG(data_container.IG['Unmeth'])), + columns=['poobah_pval']) + pII = pd.DataFrame( + index=data_container.II.index, + data=1-np.maximum(funcG(data_container.II['Meth']), funcR(data_container.II['Unmeth'])), + columns=['poobah_pval']) + # pval output: index is IlmnID; and threre's one column, 'poobah_pval' with p-values + pval = pd.concat([pIR,pIG,pII]) + return pval + +""" DEPRECATED FUNCTIONS ( container.poobah_sig ].index if isinstance(pval_probes_df, pd.DataFrame) else [] qmask = quality_mask_df.loc[ quality_mask_df['quality_mask'].isna() ].index if isinstance(quality_mask_df, pd.DataFrame) else [] @@ -54,6 +58,15 @@ def preprocess_noob(container, offset=15, pval_probes_df=None, quality_mask_df=N Gmeth = list(container.oobG['Meth'].drop(index=pval, errors='ignore').drop(index=qmask, errors='ignore')) Gunmeth = list(container.oobG['Unmeth'].drop(index=pval, errors='ignore').drop(index=qmask, errors='ignore')) oobG = pd.DataFrame( Gmeth + Gunmeth, columns=['mean_value']) + # minfi test + # ref fg_green = 442614 | vs ibG 442672 = 396374 + 46240 + # ref fg_red = 528410 | vs ibR 528482 = 439279 + 89131 + # ref oob_green = 178374 + # ref oob_red = 92578 + #oobR = pd.DataFrame( data={'mean_value': container.oobR['Meth']}) + #oobG = pd.DataFrame( data={'mean_value': container.oobG['Unmeth']}) + #print(f" oobR {oobR.shape} oobG {oobG.shape}") + #import pdb;pdb.set_trace() debug_warnings = "" if oobR['mean_value'].isna().sum() > 0: @@ -89,8 +102,19 @@ def preprocess_noob(container, offset=15, pval_probes_df=None, quality_mask_df=N noob_green = ibG_nl.round({'bg_corrected':0}) noob_red = ibR_nl.round({'bg_corrected':0}) - # by default, this is turned off because sesame uses non-linear dye-bias-correction. - if linear_dye_correction == True: + if unit_test_oob: + return { + 'oobR': oobR, + 'oobG': oobG, + 'noob_green': noob_green, + 'noob_red': noob_red, + } + + # by default, this last step is omitted for sesame + if nonlinear_dye_correction == True: + # update() expects noob_red/green to have IlmnIDs in index, and contain bg_corrected for ALL probes. + container.update_probe_means(noob_green, noob_red) + elif nonlinear_dye_correction == False: # this "linear" method may be anologous to the ratio quantile normalization described in Nature: https://www.nature.com/articles/s41598-020-72664-6 normexp_bg_correct_control(container.ctrl_green, params_green) normexp_bg_correct_control(container.ctrl_red, params_red) @@ -100,27 +124,13 @@ def preprocess_noob(container, offset=15, pval_probes_df=None, quality_mask_df=N avg_red = container.ctrl_red[mask_red]['bg_corrected'].mean() rg_ratios = avg_red / avg_green red_factor = 1 / rg_ratios - if unit_test_oob: - return { - 'oobR': oobR, - 'oobG': oobG, - 'noob_green': noob_green, - 'noob_red': noob_red, - } container.update_probe_means(noob_green, noob_red, red_factor) container._SigSet__minfi_noob = True - else: - # update() expects noob_red/green to have IlmnIDs in index, and contain bg_corrected for ALL probes. - if unit_test_oob: - return { - 'oobR': oobR, - 'oobG': oobG, - 'noob_green': noob_green, - 'noob_red': noob_red, - } - container.update_probe_means(noob_green, noob_red) + elif nonlinear_dye_correction is None: if debug: - LOGGER.warning("could not update container methylated / unmethylated noob values, because preprocess_sesame_noob has already run once.") + LOGGER.info("skipping linear/nonlinear dye-bias correction step") + # skips the minfi-linear step and won't trigger the sesame nonlinear dye bias step downstream, if you REALLY want it uncorrected. Mostly for debugging / benchmarking. + container.update_probe_means(noob_green, noob_red) class BackgroundCorrectionParams(): @@ -157,6 +167,7 @@ def normexp_bg_corrected(fg_probes, ctrl_probes, offset, sample_name=None): corrected_signals = apply_bg_correction(fg_means, params) fg_probes['bg_corrected'] = corrected_signals + fg_probes['bg_corrected'] = fg_probes['bg_corrected'].round(1) return fg_probes, params @@ -292,18 +303,17 @@ def _apply_sesame_quality_mask(data_container): -###### DEPRECATED ##### - +""" ##### DEPRECATED (