update deck position and mix vol in extraction protocol

DAMPLAB · Oct 16, 2020 · 2cbe1e0 · 2cbe1e0
1 parent f95202b
commit 2cbe1e0
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Showing 2 changed files with 6 additions and 6 deletions.
diff --git a/rna_extraction_magmax/rna_constants.py b/rna_extraction_magmax/rna_constants.py
@@ -52,11 +52,11 @@
 }, {
     'NAME': 'opentrons_96_tiprack_300ul',
     'SLOT': 9,
-    'LABEL': 'Filter Tip LG7'
+    'LABEL': 'Filter Tip LG9'
 }, {
     'NAME': 'opentrons_96_tiprack_300ul',
     'SLOT': 11,
-    'LABEL': 'Filter Tip LG9'
+    'LABEL': 'Filter Tip LG11'
 }]
 
 # Instruments

diff --git a/rna_extraction_magmax/rna_extraction_magmax.py b/rna_extraction_magmax/rna_extraction_magmax.py
@@ -264,10 +264,10 @@ def add_beads(num_cols=1, pipette=None, source=[], dest=[]):
         reagent_vol -= vol_transfer
 
 def add_ms2(num_cols=1, pipette=None, source=None, dest=[]):
-    for c in range(num_cols):
-        transfer(vol=VOL_PK, pipette=None,
-                 source=source[0], dest=dest[c][0],
-                 mix_before=(2, VOL_10), mix_after=(3, VOL_SAMPLE),
+    for c in range(2):
+        transfer(vol=VOL_PK, pipette=p20,
+                 source=source, dest=dest[c],
+                 mix_before=(2, VOL_10), mix_after=(3, VOL_10),
                  touch_tip=(TOUCH_RADIUS_SM_LG, TOUCH_RADIUS_SM_LG))
 
 def discard_supernatant(num_cols=1, pipette=None, source=[], dest=[], vol=0):