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Flow Cytometry Analysis of an In Situ PCR for the Detection of Human Immunodeficiency Virus Type-1 (HIV-1) Proviral DNA

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PRINS and In Situ PCR Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 71))

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Abstract

The polymerase chain reaction (PCR) (1,2) is extremely sensitive and flexible, and in theory, will detect a single copy of a specific DNA (or retrotranscribed RNA) sequence either in cell cultures or in clinical samples (3). PCR technology has, therefore, been applied to the diagnosis of a wide range of clinical conditions, in particular, infectious diseases (46

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© 1997 Humana Press Inc, Totowa, NJ

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Gibellini, D.E., Re, M.C., Furlini, G., La Placa, M. (1997). Flow Cytometry Analysis of an In Situ PCR for the Detection of Human Immunodeficiency Virus Type-1 (HIV-1) Proviral DNA. In: Gosden, J.R. (eds) PRINS and In Situ PCR Protocols. Methods in Molecular Biology™, vol 71. Humana Press. https://doi.org/10.1385/0-89603-395-3:113

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  • DOI: https://doi.org/10.1385/0-89603-395-3:113

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-395-5

  • Online ISBN: 978-1-59259-557-0

  • eBook Packages: Springer Protocols

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