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Molecular characterization of a novel victorivirus isolated from Botryosphaeria dothidea, the causal agent of longan leaf spot disease

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Abstract

Mycoviruses are widespread in all major taxonomic groups of filamentous fungi. Previous research has indicated that mycoviruses are associated with the phytopathogenic fungus Botryosphaeria dothidea. In this study, three distinct double-stranded RNA viruses were detected in B. dothidea strain YCLYY11 isolated from a leaf spot of longan (Dimocarpus longana). The results of BLAST analysis revealed that the predicted amino acid sequences of those viruses were similar to those of Botryosphaeria dothidea chrysovirus 1, Botryosphaeria dothidea partitivirus 1, and an apparent novel victorivirus. Sequencing and analysis of the complete genome of the novel victorivirus indicated it is 5218 bp in length and contains two open reading frames (ORFs) that overlap at the tetranucleotide AUGA. BLASTp analysis of the proteins encoded by ORF1 and ORF2 showed that they were most similar to the coat protein and RNA-dependent RNA polymerase of Sphaeropsis sapinea RNA virus 2 (81.37% and 74.09% identical, respectively). A phylogenetic tree showed that the novel virus clustered together with victoriviruses and was separate from members of the other four genera of the family Totiviridae. Based on its genome structure and the results of phylogenetic analysis, we propose that this novel victorivirus should be named "Botryosphaeria dothidea victorivirus 3". This is also the first report of these three mycoviruses coinfecting a strain of B. dothidea.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (32072476), the Natural Science Research Project of Chongqing Science and Technology (cstc2019jcyj-msxmX0200), the Science and Technology Research Program of Chongqing Municipal Education Commission (KJQN202001411, KJQN202001441), the  China Litchi and Longan Industry Technology Research System (CARS-32), and the Research Funding Project of Yangtze Normal University (2017KYQD54). We also thank Dr. Mengmeng Yang (Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China) for providing data from deep sequencing used for confirming the name of BdVV3. The authors thank AiMi Academic Services (www.aimieditor.com) for the English language editing and review services.

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Authors and Affiliations

  1. College of Biology and Food Engineering, Chongqing Three Gorges University, Chongqing, 404100, People’s Republic of China

    Jiali Li, Lifeng Zhai & Ming Liu

  2. College of Life Science and Biotechnology, Yangtze Normal University, Chongqing, 408100, People’s Republic of China

    Lifeng Zhai, Meixin Zhang, Gangjun Luo, Yongqi Wen, Tingting Cao, Huan Xia & Jiayu Zhang

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  1. Jiali Li
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Li, J., Zhai, L., Zhang, M. et al. Molecular characterization of a novel victorivirus isolated from Botryosphaeria dothidea, the causal agent of longan leaf spot disease. Arch Virol 167, 2417–2422 (2022). https://doi.org/10.1007/s00705-022-05573-w

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