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Automated Flow Cytometric Analysis of Drug Susceptibility of Malaria Parasites

Philip H. Van Vianen University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand

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Sodsri Thaithong University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand

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Paul P. Reinders University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand

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Anneloes Van Engen University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand

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Maarten Van Der Keur University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand

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Hans J. Tanke University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand

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Hugo J. Van Der Kaay University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand

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Barend Mons University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand

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DOI:
https://doi.org/10.4269/ajtmh.1990.43.602
Volume/Issue:
Volume 43: Issue 6
Page(s):
602–607
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A method is described for the fully automated reading of Plasmodium falciparum drug susceptibility tests. Cultured material was fixed and could be stored for ≥6 months until analysis. The parasites were stained for DNA with the fluorescent dye Hoechst 33258 and analyzed by flow cytometry. The procedure was done in 96-well microtiter plates, after which the material was directed through the sensing region in the flow cytometer. The data resulting from the analysis were stored by microcomputer and processed by a program developed for this purpose. Using this method, a number of different parameters describing the growth in culture can be assessed.

Author Notes

Copyright:
Copyright © 1990 by The American Society of Tropical Medicine and Hygiene 1990
Published Online:
Dec 1990
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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