Structural changes in gut microbiome after Ramadan fasting: a pilot study
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It has been largely accepted that dietary changes have an effect on gut microbial composition. In this pilot study we hypothesised that Ramadan fasting, which can be considered as a type of time-restricted feeding may lead to changes in gut microbial composition and diversity. A total of 9 adult subjects were included in the study. Stool samples were collected before (baseline) and at the end of the Ramadan fasting (after 29 days). Following the construction of an 16S rRNA amplicon library, the V4 region was sequenced using the Illumina Miseq platform. Microbial community analysis was performed using the QIIME program. A total of 27,521 operational taxonomic units (OTUs) with a 97% similarity were determined in all of the samples. Microbial richness was significantly increased after Ramadan according to observed OTU results (P=0.016). No significant difference was found in terms of Shannon index or phylogenetic diversity metrics of alpha diversity. Microbial community structure was significantly different between baseline and after Ramadan samples according to unweighted UniFrac analysis (P=0.025). LEfSe analysis revealed that Butyricicoccus, Bacteroides, Faecalibacterium, Roseburia, Allobaculum, Eubacterium, Dialister and Erysipelotrichi were significantly enriched genera after the end of Ramadan fasting. According to random forest analysis, the bacterial species most affected by the Ramadan fasting was Butyricicoccus pullicaecorum. Despite this is a pilot study with a limited sample size; our results clearly revealed that Ramadan fasting, which represents an intermittent fasting regime, leads to compositional changes in the gut microbiota.
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Alzoghaibi, M.A., Pandi-Perumal, S.R., Sharif, M.M. and BaHammam, A.S., 2014. Diurnal intermittent fasting during Ramadan: the effects on leptin and ghrelin levels. PLoS ONE 9: e92214. https://doi.org/10.1371/journal.pone.0092214
-
Backhed, F., Ding, H., Wang, T., Hooper, L.V., Koh, G.Y., Nagy, A., Semenkovich, C.F. and Gordon, J.I., 2004. The gut microbiota as an environmental factor that regulates fat storage. Proceedings of the Nationall Academy of Sciences of the USA 101: 15718-15723. https://doi.org/10.1073/pnas.0407076101
-
Beli, E., Yan, Y., Moldovan, L., Vieira, C.P., Gao, R., Duan, Y., Prasad, R., Bhatwadekar, A., White, F.A., Townsend, S.D., Chan, L., Ryan, C.N., Morton, D., Moldovan, E.G. Chu, F.I., Oudit, G.Y., Derendorf, H., Adorini, L., Wang, X.X., Evans-Molina, C., Mirmira, R.G., Boulton, M.E., Yoder, M.C., Li, Q., Levi, M., Busik, J.V. and Grant, M.B., 2018. Restructuring of the gut microbiome by intermittent fasting prevents retinopathy and prolongs survival in db/db mice. Diabetes 67: 1867-1879. https://doi.org/10.2337/db18-0158
-
Caporaso, J.G., Kuczynski, J., Stombaugh, J., Bittinger, K., Bushman, F.D., Costello, E.K., Fierer, N., Pena, A.G., Goodrich, J.K., Gordon, J.I., Huttley, G.A., Kelley, S.T., Knights, D., Koenig, J.E., Ley, R.E., Lozupone, C.A., McDonald, D., Muegge, B.D., Pirrung, M., Reeder, J., Sevinsky, J.R., Turnbaugh, P.J., Walters, W.A., Widmann, J., Yatsunenko, T., Zaneveld, J. and Knight, R., 2010. QIIME allows analysis of high-throughput community sequencing data. Nature Methods 7: 335-336. https://doi.org/10.1038/nmeth.f.303
-
Carlson, O., Martin, B., Stote, K.S., Golden, E., Maudsley, S., Najjar, S.S., Ferrucci, L., Ingram, D.K., Longo, D.L., Rumpler, W.V., Baer, D.J., Egan, J. and Mattson, M.P., 2007. Impact of reduced meal frequency without caloric restriction on glucose regulation in healthy, normal-weight middle-aged men and women. Metabolism 56: 1729-1734. https://doi.org/10.1016/j.metabol.2007.07.018
-
Derrien, M., Vaughan, E.E., Plugge, C.M. and De Vos, W.M., 2004. Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium. International Journal of Systematic and Evolutionary Microbiology 54: 1469-1476. https://doi.org/10.1099/ijs.0.02873-0
-
Everard, A., Belzer, C., Geurts, L., Ouwerkerk, J.P., Druart, C., Bindels, L.B., Guiot, Y., Derrien, M., Muccioli, G.G., Delzenne, N.M., De Vos, W.M. and Cani, P.D., 2013. Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity. Proceedings of the National Academy of Sciences of the USA 110: 9066-9071. https://doi.org/10.1073/pnas.1219451110
-
Faith, J.J., Guruge, J.L., Charbonneau, M., Subramanian, S., Seedorf, H., Goodman, A.L., Clemente, J.C., Knight, R., Heath, A.C., Leibel, R.L., Rosenbaum, M. and Gordon, J.I., 2013. The long-term stability of the human gut microbiota. Science 341: 1237439. https://doi.org/10.1126/science.1237439
-
Fontana, L. and Partridge, L., 2015. Promoting health and longevity through diet: from model organisms to humans. Cell 161: 106-118. https://doi.org/10.1016/j.cell.2015.02.020
-
Geirnaert, A., Calatayud, M., Grootaert, C., Laukens, D., Devriese, S., Smagghe, G., De Vos, M., Boon, N. and Van de Wiele, T., 2017. Butyrate-producing bacteria supplemented in vitro to Crohn’s disease patient microbiota increased butyrate production and enhanced intestinal epithelial barrier integrity. Scientific Reports 7: 11450. https://doi.org/10.1038/s41598-017-11734-8
-
Guarner, F. and Malagelada, J.R., 2003. Gut flora in health and disease. Lancet 361: 512-519. https://doi.org/10.1016/S0140-6736(03)12489-0
-
Haas, J.T. and Staels, B., 2017. Fasting the microbiota to improve metabolism? Cell Metabolism 26: 584-585. https://doi.org/10.1016/j.cmet.2017.09.013
-
Hatori, M., Vollmers, C., Zarrinpar, A., DiTacchio, L., Bushong, E.A., Gill, S., Leblanc, M., Chaix, A., Joens, M., Fitzpatrick, J.A., Ellisman, M.H. and Panda, S., 2012. Time-restricted feeding without reducing caloric intake prevents metabolic diseases in mice fed a high-fat diet. Cell Metabolism 15: 848-860. https://doi.org/10.1016/j.cmet.2012.04.019
-
Le Bastard, Q., Ward, T., Sidiropoulos, D., Hillmann, B.M., Chun, C.L., Sadowsky, M.J., Knights, D. and Montassier, E., 2018. Fecal microbiota transplantation reverses antibiotic and chemotherapy-induced gut dysbiosis in mice. Scientific Reports 8: 6219. https://doi.org/10.1038/s41598-018-24342-x
-
Ley, R.E., Backhed, F., Turnbaugh, P., Lozupone, C.A., Knight, R.D. and Gordon, J.I., 2005. Obesity alters gut microbial ecology. Proceedings of the National Academy of Sciences of the USA 102: 11070-11075. https://doi.org/10.1073/pnas.0504978102
-
Li, G., Xie, C., Lu, S., Nichols, R.G., Tian, Y., Li, L., Patel, D., Ma, Y., Brocker, C.N., Yan, T., Krausz, K.W., Xiang, R., Gavrilova, O., Patterson, A.D. and Gonzalez, F.J., 2017. Intermittent fasting promotes white adipose browning and decreases obesity by shaping the gut microbiota. Cell Metabolism 26: 672-685. https://doi.org/10.1016/j.cmet.2017.08.019
-
Machiels, K., Joossens, M., Sabino, J., De Preter, V., Arijs, I., Eeckhaut, V., Ballet, V., Claes, K., Van Immerseel, F., Verbeke, K., Ferrante, M., Verhaegen, J., Rutgeerts, P. and Vermeire, S., 2014. A decrease of the butyrate-producing species Roseburia hominis and Faecalibacterium prausnitzii defines dysbiosis in patients with ulcerative colitis. Gut 63: 1275-1283. https://doi.org/10.1136/gutjnl-2013-304833
-
Patterson, R.E. and Sears, D.D., 2017. Metabolic effects of intermittent fasting. Annual Review of Nutrition 37: 371-393. https://doi.org/10.1146/annurev-nutr-071816-064634
-
Remely, M., Hippe, B., Geretschlaeger, I., Stegmayer, S., Hoefinger, I. and Haslberger, A., 2015. Increased gut microbiota diversity and abundance of Faecalibacterium prausnitzii and Akkermansia after fasting: a pilot study. Wiener Klinische Wochenschrift 127: 394-398. https://doi.org/10.1007/s00508-015-0755-1
-
Round, J.L. and Mazmanian, S.K., 2009. The gut microbiota shapes intestinal immune responses during health and disease. Nature Reviews Immunology 9: 313-323. https://doi.org/10.1038/nri2515
-
Santacruz, A., Collado, M.C., Garcia-Valdes, L., Segura, M.T., Martin-Lagos, J.A., Anjos, T., Marti-Romero, M., Lopez, R.M., Florido, J., Campoy, C. and Sanz, Y., 2010. Gut microbiota composition is associated with body weight, weight gain and biochemical parameters in pregnant women. British Journal of Nutrition 104: 83-92. https://doi.org/10.1017/S0007114510000176
-
Sanz, Y., Santacruz, A. and De Palma, G., 2008. Insights into the roles of gut microbes in obesity. Interdisciplinary Perspectives on Infectious Diseases 2008: 829101. https://doi.org/10.1155/2008/829101
-
Segata, N., Izard, J., Waldron, L., Gevers, D., Miropolsky, L., Garrett, W.S. and Huttenhower, C., 2011. Metagenomic biomarker discovery and explanation. Genome Biology 12: R60. https://doi.org/10.1186/gb-2011-12-6-r60
-
Sokol, H., Pigneur, B., Watterlot, L., Lakhdari, O., Bermudez-Humaran, L.G., Gratadoux, J.J., Blugeon, S., Bridonneau, C., Furet, J.P., Corthier, G., Grangette, C., Vasquez, N., Pochart, P., Trugnan, G., Thomas, G., Blottiere, H.M., Dore, J., Marteau, P., Seksik, P. and Langella, P., 2008. Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients. Proceedings of the National Academy of Sciences of the USA 105: 16731-16736. https://doi.org/10.1073/pnas.0804812105
-
Sonnenburg, J.L., Xu, J., Leip, D.D., Chen, C.H., Westover, B.P., Weatherford, J., Buhler, J.D. and Gordon, J.I., 2005. Glycan foraging in vivo by an intestine-adapted bacterial symbiont. Science 307: 1955-1959. https://doi.org/10.1126/science.1109051
-
Tamanai-Shacoori, Z., Smida, I., Bousarghin, L., Loreal, O., Meuric, V., Fong, S.B., Bonnaure-Mallet, M. and Jolivet-Gougeon, A., 2017. Roseburia spp.: a marker of health? Future Microbiology 12: 157-170. https://doi.org/10.2217/fmb-2016-0130
-
Turnbaugh, P.J., Hamady, M., Yatsunenko, T., Cantarel, B.L., Duncan, A., Ley, R.E., Sogin, M.L., Jones, W.J., Roe, B.A., Affourtit, J.P., Egholm, M., Henrissat, B., Heath, A.C., Knight, R. and Gordon, J.I., 2009. A core gut microbiome in obese and lean twins. Nature 457: 480-484. https://doi.org/10.1038/nature07540
-
Turnbaugh, P.J., Ley, R.E., Mahowald, M.A., Magrini, V., Mardis, E.R. and Gordon, J.I., 2006. An obesity-associated gut microbiome with increased capacity for energy harvest. Nature 444: 1027-1031. https://doi.org/10.1038/nature05414
-
Wei, S., Han, R., Zhao, J., Wang, S., Huang, M., Wang, Y. and Chen, Y., 2018. Intermittent administration of a fasting-mimicking diet intervenes in diabetes progression, restores beta cells and reconstructs gut microbiota in mice. Nutrition and Metabolism 15: 80. https://doi.org/10.1186/s12986-018-0318-3
-
Wexler, A.G. and Goodman, A.L., 2017. An insider’s perspective: bacteroides as a window into the microbiome. Nature Microbiology 2: 17026. https://doi.org/10.1038/nmicrobiol.2017.26
-
Zarrinpar, A., Chaix, A., Yooseph, S. and Panda, S., 2014. Diet and feeding pattern affect the diurnal dynamics of the gut microbiome. Cell Metabolism 20: 1006-1017. https://doi.org/10.1016/j.cmet.2014.11.008
Supplementary Materials
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Structural changes in gut microbiome after Ramadan fasting: a pilot study
In: Beneficial MicrobesSearch for other papers by C. Ozkul in
Current site
Google Scholar
PubMed
Search for other papers by M. Yalinay in
Current site
Google Scholar
PubMed
Search for other papers by T. Karakan in
Current site
Google Scholar
PubMed
It has been largely accepted that dietary changes have an effect on gut microbial composition. In this pilot study we hypothesised that Ramadan fasting, which can be considered as a type of time-restricted feeding may lead to changes in gut microbial composition and diversity. A total of 9 adult subjects were included in the study. Stool samples were collected before (baseline) and at the end of the Ramadan fasting (after 29 days). Following the construction of an 16S rRNA amplicon library, the V4 region was sequenced using the Illumina Miseq platform. Microbial community analysis was performed using the QIIME program. A total of 27,521 operational taxonomic units (OTUs) with a 97% similarity were determined in all of the samples. Microbial richness was significantly increased after Ramadan according to observed OTU results (P=0.016). No significant difference was found in terms of Shannon index or phylogenetic diversity metrics of alpha diversity. Microbial community structure was significantly different between baseline and after Ramadan samples according to unweighted UniFrac analysis (P=0.025). LEfSe analysis revealed that Butyricicoccus, Bacteroides, Faecalibacterium, Roseburia, Allobaculum, Eubacterium, Dialister and Erysipelotrichi were significantly enriched genera after the end of Ramadan fasting. According to random forest analysis, the bacterial species most affected by the Ramadan fasting was Butyricicoccus pullicaecorum. Despite this is a pilot study with a limited sample size; our results clearly revealed that Ramadan fasting, which represents an intermittent fasting regime, leads to compositional changes in the gut microbiota.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1053 | 900 | 149 |
| Full Text Views | 24 | 20 | 6 |
| PDF Views & Downloads | 46 | 43 | 8 |