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Vascular Smooth Muscle Cells

Isolation, Culture, and Characterization

  • Protocol
Angiogenesis Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 46))

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Abstract

In blood vessel development (vasculogenesis) smooth muscle cells (SMCs) are derived by differentiation of mesenchymal cells under the influence of mediators secreted by the endothelial cells (ECs) composing newly formed vessels. In angiogenesis, SMCs can be formed in the same way or by proliferation of existing SMCs (1). Key events in the development of atherosclerotic lesions and restenosis of arteries are now recognized to include vascular SMC migration, hyperplasia, and hypertrophy (2). This has led to an increase in studies of SMC function in response to growth factors, extracellular matrix, and lipoproteins, under controlled in vitro conditions, to address the cellular mechanisms in atherogenesis. Most of the methodology used for vascular smooth muscle culture has been developed for such studies (3).

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Author information

Authors and Affiliations

  1. Division of Cardiovascular Medicine,,School of Clinical Medicine, University of Cambridge, Cambridge, UK

    Richard C. M. Siow

  2. Centre for Cardiovascular Biology and Medicine, School of Biomedical Sciences,King’ s College London, London, UK

    Jeremy D. Pearson

Authors
  1. Richard C. M. Siow
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  2. Jeremy D. Pearson
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Editor information

Editors and Affiliations

  1. University of Nottingham and City Hospital, Nottingham, UK

    J. Clifford Murray

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© 2001 Humana Press Inc.

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Siow, R.C.M., Pearson, J.D. (2001). Vascular Smooth Muscle Cells. In: Murray, J.C. (eds) Angiogenesis Protocols. Methods in Molecular Medicine™, vol 46. Humana Press. https://doi.org/10.1385/1-59259-143-4:237

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  • DOI: https://doi.org/10.1385/1-59259-143-4:237

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-698-7

  • Online ISBN: 978-1-59259-143-5

  • eBook Packages: Springer Protocols

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