Abstract
The stable RNA molecules (rRNA, tRNA, and sn[o]RNA) of the cell are subjected to nucleotide modifications on the way to the functionally mature state (see refs. 1–3). These modifications, which account for up to 16% of the nucleotides in higher eucaryotic rRNAs, are introduced at the poly nucleotide level at specific sites. They include nucleosides methylated either on the base or on the 2′-hydroxyl of ribose, pseudouridine (ψ; 5-β-D-ribofuranosyluracil) and its derivatives such as 1-methyl-3-(3-amino-3-carboxypropyl)pseudouridine in eukaryotic small subunit rRNA, dihydrouridine, N4-acetylcytidine and a number of more complex nucleotides found in tRNA molecules (4). Some of these modified bases are shown Fig. 1.
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Bakin, A.V., Ofengand, J. (1998). Mapping of Pseudouridine Residues in RNA to Nucleotide Resolution. In: Martin, R. (eds) Protein Synthesis. Methods in Molecular Biology, vol 77. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-397-X:297
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DOI: https://doi.org/10.1385/0-89603-397-X:297
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