The Depsipeptide Romidepsin Reverses HIV-1 Latency In Vivo
Fig 2
Romidepsin induced HIV-1 transcription and presence of extracellular viral RNA.
Panel (A) shows mean (SEM) levels of H3 acetylation measured by flow cytometry in lymphocytes. (B) shows mean (SEM) change from baseline in the level of CA US HIV-1 RNA. The first sample time point after each of the 3 romidepsin doses is approximately ½ hour after end of the 4 hour infusion. (C) shows individual levels of plasma HIV-1 RNA, determined using the Roche Cobas Taqman assay, while panel (D) shows mean plasma HIV-1 RNA data for all 6 participants determined using a Transcription-Mediated Amplication assay. (E) is an overlay of H3 acetylation and CA US HIV-1 RNA data presented in Panels A and B. (F) is an overlay of CA US HIV-1-RNA (B) and plasma HIV-1 RNA (E). SEM, standard error of mean; CA US HIV-1-RNA: cell-associated unspliced HIV-1 RNA; LoQ, limit of quantitation; TMA, Transcription-Mediated Amplication. Statistical comparisons were performed using Wilcoxon matched-pairs signed-ranks test, Asterisk indicate p<0.05.