Inflammatory Monocytes Orchestrate Innate Antifungal Immunity in the Lung
Figure 6
CCR2+Mo differentiate into Mo-DC and efficiently kill A.fumigatus.
CCR2 reporter mice were infected with FLARE conidia and lung cell suspensions were enumerated and examined by (A) imaging cytometry and (B–H) flow cytometry. (A) Imaging cytometry of lung GFP+ (CCR2+) cells from FLARE-infected CCR2 reporter mice 36 h p.i. The micrograph depicts dsRed+AF633+ and dsRed−AF633+ monocytes that contain live and killed conidia, respectively. BF, bright-field. (B–E) The graphs show the total number (mean ± SEM) of lung CCR2+Mo (white circles) and Mo-DCs (black circles) at the indicated times p.i. . CCR2+Mo (white circles), and Mo-DC (black circles) were identified as shown in Figure S1. (B) Data shows total recrutiment of each subset over time. (C) The graph shows the total number of CCR2+Mo (white circles) or Mo-DCs (black circles) that contain engulfed conidia. (D–E) The graph shows the total number of CCR2+Mo (white circles) or Mo-DCs (black circles) that contain (D) live or (E) killed conidia. (F–H) Comparison of CCR2+Mo, Mo-DC and neutrophil conidiacidal activity. The scatter plots show the frequency of fungus-engaged (F) CCR2+Mo, (G) Mo-DC, and (H) neutrophils that contain live (red circles) or killed (blue circles) FLARE conidia at the indicated times p.i. Results are pooled from two experiments. (I) The graph shows lung CFUs from DT-treated B6 controls (white circles), DT-treated CCR2 depleter (black squares), and anti-Ly6G-treated B6 mice (grey triangles) at day +1 p.i.. Each symbol represents one mouse. Results are for one experiment representative of two individual experimenst for all data shown in this figure. Statistical analysis was performed using a Mann Whitney test.