CD11b+, Ly6G+ Cells Produce Type I Interferon and Exhibit Tissue Protective Properties Following Peripheral Virus Infection
Figure 4
Ly6C+Ly6G- and Ly6C+Ly6G+ cells have distinct functional profiles.
Mice were infected i.d. in the ear pinnae with VACV. Ear pinnae were harvested at 5 days post-infection. The Ly6C+Ly6G- (top, open histograms) and Ly6C+Ly6G+ (bottom, open histograms) CD11b+ monocyte subpopulations were analyzed for the expression of various cytokines/effector molecules using antibodies (A-D) or a reactive dye triggered by exposure to ROS (E) and compared to CD11b- cells (filled histograms). For TNF-α staining, cells were first incubated with CpG DNA. Data are representative of at least three independent experiments in which the phenotype of at least two mice were examined independently.