Asymmetric Deactivation of HIV-1 gp41 following Fusion Inhibitor Binding
Figure 2
5-Helix and C37 inhibition of primary isolate HIV-1 strain JR-FL.
IC50 values for the wild type and lower affinity variants were determined for HIV-1JR-FL infections of HOS-CD4-CCR5 cells (gray bars). For comparison, the IC50 values for HIV-1HXB2 infections of HOS-CDR-CXCR4 cells are also shown (hatched bars). The numbers below the axis labels are KD values (in nM) measured for binding to the HXB2 sequence. The arrow indicates an IC50 value in excess of 20 µM.