Interaction of Polymerase Subunit PB2 and NP with Importin α1 Is a Determinant of Host Range of Influenza A Virus
Figure 2
Interaction of PB2 and NP with Importin α1 in Avian Cells
CEC-32 cells were transfected with plasmids encoding SC35-PB2 (30 μg), SC35M-PB2 (30 μg), and SC35-PB2 D701N (30 μg) (A) and SC35-NP (10 μg) and SC35M-NP (10 μg) (B) proteins. As a control, non-transfected cells were used (Mock). 48 h after transfection cells were harvested and immunoprecipitated using the respective PB2 or NP antibody. Precipitated eluates (1–3) and non-precipitated whole cell lysates of transfected cells were subjected to SDS gel electrophoresis, and PB2, NP, and importin α1 were detected by Western blot analysis. Importin α1 bound to PB2 (C) and NP (D) was quantitated as described in the legend of Figure 1.