LL-37 alone and in combination with IL17A enhances proinflammatory cytokine expression in parallel with hyaluronan metabolism in human synovial sarcoma cell line SW982—A step toward understanding the development of inflammatory arthritis
Fig 6
Effects of LL-37 and its combination with IL17A on apoptosis, caspase 8 activity, and cell cycle.
SW982 cells were untreated (Ctrl) or treated with 12.5 ng/mL of LL-37 and/or 5 or 10 ng/mL of IL17A for 48 hr before being analyzed. (A and B) Cells were assayed for caspase3/7 activity by a flow cytometer with 5% DMSO as a positive control. (C) Caspase 8 activity was measured by ELISA. (D and E) Cells were stained with PI and checked for cell cycle by a flow cytometer. FBS was used as a positive control. The values from triplicate or duplicate experiments are expressed as the mean ± SD of fold increases compared to the untreated control or percentage. Horizontal brackets indicate significance levels at *p < 0.05.